Isolation of 2-Chlorobenzimidazole From Melia Dubia Leaf Extract and Its Structural Characterisation

Abstract

Objective: Natural products from medicinal plants, either as isolated compounds or as standardized plant extracts exhibit promising source of medicinal activity against various diseases. The aim of the present work was to make an attempt of isolation of bioactive principle and characterization of the isolated compound, from the medicinal plant Melia dubai Methods: The extraction was done by a cold percolation method and the compound was separated and isolated by chromatography technique such as a thin layer chromatography (TLC), column chromatography and high-performance liquid chromatography (HPLC). The isolated compound was crystallized and the structural characterization of the isolated compound was made using UV-Visible, FT-IR, 1Results: The separated and isolated compound was characterized by both physical and spectral methods like Ultraviolet-Visible spectroscopy (UV-Visible), Fourier transform infrared spectroscopy (FT-IR), Proton Nuclear Magnetic Resonance Spectroscopy (1H-NMR), Gas chromatography-mass spectrometry (GC-MS), and Mass spectrometry(MS). Based on the studies, organizational characteristics of one bioactive principle were deciphered. The results revealed that the isolated species is 2-chlorobenzimidazole and it agreed well with the reported value and spectra for 2-chlorobenzimidazole.H-NMR, GC-MS and MS techniques which confirmed the structure of the isolated compound. Conclusion:The above results obtained in this research work clearly indicated the promising occurrence of 2-chlorobenzimidazole in Media dubia plant leaves. The future scope of these studies may guide us to view the biological activity of the isolated compound.

Mitragynine Percentages of Various Kratom Variants Seized in Indonesia: A Quantitative Analysis Using Liquid Chromatography-Photo Diode Array Detector

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Isolation, Characterization and Antioxidant Activity of Plumbago indica L. Extract

“…The spots were visualized in Visible light, UV light and iodine chamber and Rf values were calculated. 16 Flash Chromatography Solvent system was optimized using TLC. BUCHI Flash Chromatography-MPLC Cartridges 12g Silica 40-63 µm was used.…”

Melia dubia Cav. Meliaceae

“…Studies of leaf extract of M. dubia revealed 44 phytochemicals by chromatographic techniques. Some of the major compounds listed are alkaloids, flavonoids, glycosides, limonoids, resins, oleoresins, tannins, saponins, steroids, terpenoids, phenolic derivatives, lipophilic organic compounds, linolenic acid, palmitic acid, caryophyllene, humulene, aromadendrene, probucol, germacrene-D, phthalic acid 6-ethyl-3-octyl, and butylated hydroxy toluene (Zhao et al 2010;Valentina et al 2013;Murugesan et al 2013;Jeyaleela et al 2017). Essential oils found in leaves are mainly monoterpenes (α-pinene, β-pinene, sabinene), oxygenated monoterpenes (bicyclic ketone camphor, iso-borneol, borneol), smaller amount of alkanes, sesquiterpene, hydrocarbons, and phenylpropanoids (Nagalakshmi et al 2001).…”