Medicinal plants engrossed great attention for the synthesis of new chemical entities and its biomolecules has fabulous application in many fields such as corrosion, nanotechnology, pharmaceutical companies, and cosmetic industries. The main purpose of the present work is biosynthesis of zinc oxide nanoparticles via simple homogeneous precipitation method using the isolated flavonoid quercetin, which was derived from the medicinal plant Combretum ovalifolium. Antioxidant activity and photocatalytic degradation of methylene blue dye were studied on the synthesized ZnONPs. Zinc acetate dihydrate (Zn (CH 3 COO) 2 .2H 2 O) was utilized as a precursor and quercetin as a reducing agent. Formed Quercetin mediated zinc oxide nanoparticles (Q-ZnONPs) were characterized by UV-Visible, FT-IR, XRD, EDX, and SEM. Fiber shaped Q-ZnONPs were obtained with single crystalline nature, its average diameter was found to be 31.24nm. The results shows that, 168.045µg/ml of Q-ZnONPs scavenged 50% of DPPH on antioxidant activity. The degradation behaviour of the nanomaterial was analysed. The results showed that on the addition of biosynthesized Q-ZnONPs, the degradation of methylene blue increased up to 76.01% at 240 minutes.
The bioactive constituents derived from plants attract the attention of researchers due to their potential applications in the medicinal field. In this regard, the proximate analysis and the cytotoxicity study of the plant materials play an important role in the phytochemical research. In the present work, estimation of total ash, moisture content, fiber content, crude protein, and carbohydrate were carried out under proximate analysis and the antioxidant activity of the anthocyanin present in the plant material was evaluated by DPPH (2,2-diphenyl-1-picrylhydrazyl) method. The separation of anthocyanin pigment from the plant material was done by paper chromatography (PC) technique and they are characterized by UV spectrum, chemical test and the Rf values obtained from paper chromatography. This study also investigated the in vitro cytotoxicity of Pseuderanthemum reticulatum leaves by means of MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide)) assay PBMC (Peripheral Blood Mononuclear Cell). The results of the proximate analysis showed that the plant material contains 7.6% of moisture content, 16.6 % of total ash, 5.6% of crude protein, 23.0% of crude fiber, 3.82% of crude fat and 23.64 % of carbohydrate. The free radical scavenging ability of the separated anthocyanin was found to be 72.58% at 10 µg/mL. The cytotoxicity investigation showed that the aqueous extract possess the IC50 value of 161.5μg/mL. The High percentage of radical scavenging activity and low toxicity of the plant suggest that it can be extensively used for the investigation of the bioactive constituents and its applications.
In the present work, cotton fabrics were dyed using the acidified aqueous extract of Psuderanthimum
reticulatum leaves with different mordants such as ferrous sulphate, stannous chloride, copper sulphate,
potash alum and ferric alum by premordanting technique and various shades were obtained. The
washing, perspiration and light fastness properties of the dyed fabrics were carried out by standard
methods, ISO 105-C06 A2S-2010, ISO 105 E04-2013 and ISO 105 B02 Methods 3:2014, respectively.
Cotton fabrics dyed with copper sulphate and ferric alum showed excellent to very good properties
towards the entire fastness tests studied. The bioactive colourant present in the aqueous extract of
Psuderanthimum reticulatum responsible for dyeing is anthocyanin pigment and it was identified by
chemical test, UV & FTIR spectral and HPLC analysis.
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