Inhibition of inducible nitric oxide synthase by Acanthopanax senticosus extract in RAW264.7 macrophages

Lin, Qiu-Ye; Jin, Lei; Cao, Zhenhui; Xu, You-Hai

doi:10.1016/j.jep.2008.04.003

Cited by 43 publications

(21 citation statements)

References 32 publications

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“…In the present study, we found that exposure of RAW264.7 cells to LPS caused a significant increase in nO production and inOS mrna expression. This observation is consistent with the findings previously reported by other researchers (15,36). Pre-treatment with PA markedly reduced nO production and inOS mrna expression in lpS-stimulated raW264.7 cells, suggesting that pa is capable of exerting anti-inflammatory action by inhibiting inOS mrna expression.…”

Section: Discussionsupporting

confidence: 93%

Anti-inflammatory effect of patchouli alcohol isolated from Pogostemonis Herba in LPS-stimulated RAW264.7 macrophages

Xian

et al. 2011

Experimental and Therapeutic Medicine

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Abstract. pogostemonis herba has long been used in traditional Chinese medicine for the treatment of inflammationrelated disorders. patchouli alcohol (pa) isolated from pogostemonis herba is a tricyclic sesquiterpene that is known to exert a variety of pharmacological activities. the present study aimed to investigate the anti-inflammatory effect of pa on lipopolysaccharide (lpS)-stimulated raW264.7 cells. pre-treatment with pa at concentrations of 10, 20 or 40 µm dose-dependently decreased the production of tumor necrosis factor (tnF)-α, interleukin (il)-1β, il-6, nitric oxide (nO) and prostaglandin E 2 in lpS-stimulated raW264.7 cells. in addition, pa treatment also reversed the increased mrna expression of tnF-α, il-1β, il-6, inducible nitric oxide synthase (inOS) and cyclooxygenase (cOx)-2 caused by lpS in raW264.7 cells. these results indicate that pa is an important anti-inflammatory constituent of Pogostemonis Herba and that its anti-inflammatory effect may be mediated, at least in part, by down-regulation of the mrna expression of a panel of inflammatory mediators, such as TNF-α, il-1β, il-6, inOS and cOx-2.

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Section: Discussionsupporting

confidence: 93%

Anti-inflammatory effect of patchouli alcohol isolated from Pogostemonis Herba in LPS-stimulated RAW264.7 macrophages

Xian

et al. 2011

Experimental and Therapeutic Medicine

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“…In our study, we found that the exposure of RAW264.7 cells to LPS caused a significant increase in NO production, and FS reduced to a degree NO production in LPS-stimulated RAW264.7 cells, which suggested that FS had the potential of anti-inflammatory action. This observation is consistent with the findings previously reported by other researchers (Hu et al, 2008;Lin et al, 2008).…”

Section: Discussionsupporting

confidence: 94%

Effects of forsythoside on lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages

Guan¹,

Shen²,

Zhang³

et al. 2013

Afr. J. Pharm. Pharmacol.

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“…A. senticosus has been used in traditional Chinese medicine as an adaptogen due to its pharmacological effects, including anti-bacterial, anti-cancer, anti-inflammatory, anti-gout, anti-hepatitis, anti-hyperglycemic, anti-leishmanicidal, anti-oxidant, anti-pyretic effects; other effects include choleretic, hemostatic, immunostimulatory, hypocholesterolemic and radioprotectant effects (12)(13)(14)(15). It has been shown to protect against oxidative damage and exhibits anti-diabetic activity (16,17). In China, A. senticosus is used as a nutritional supplement and a sedative (18).…”

Section: Introductionmentioning

confidence: 99%

Anti‑tumour effects of polysaccharide extracted from Acanthopanaxï¿½senticosus and cell‑mediated immunity

Meng

Pan²,

Liu

et al. 2017

Exp Ther Med

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Abstract. Acanthopanax senticosus, also known as Siberian ginseng, is widely distributed throughout northern Asia and used in traditional Chinese medicine; it has been reported to prevent a number of diseases. However, the association between the antitumour and immunostimulatory activities of polysaccharide extracted from A. senticosus (ASPS) remains to be elucidated. The aim of the present study was to investigate the anti-tumour and immunomodulatory effects of polysaccharide extracted from ASPS on Crocker sarcoma S 180 , hepatic carcinoma H 22 and uterine cervical carcinoma U 14 tumour cell lines implanted in mice. High performance liquid chromatography, gas chromatography and infrared spectroscopy were used to analyse the monosaccharide composition of ASPS. The monosaccharide composition of ASPS (Arabic candy: Xylose: Glucose: Mannose) was 7.1:22.3:7.6:1.0. On day 0, female Kunming mice, were injected subcutaneously with 1x10 8 tumour cells in 0.2 ml. The inoculated mice were subsequently divided into five groups (10 mice/group) as follows: Model group, treated with normal saline; positive control group, treated with 30 mg/kg cyclophosphamide (CTX); and three treatment groups, treated with 200, 100 or 50 mg/kg ASPS. Non-inoculated mice were divided into the normal group, which was treated with normal saline, and the negative control group, which was treated with 200 mg/kg ASPS (n=10/group). CTX and ASPS were administered intragastrically once daily for 10 days. All mice were sacrificed on day 11. ASPS was observed to have an inhibitory effect on the growth of S 180 , H 22 and U 14 cells in solid and ascites tumour-bearing mice. Serum interleukin (IL)-2 and IL-12 levels were significantly increased in S 180 solid tumour-bearing mice treated with 200 or 100 mg/kg ASPS compared with mice in the normal, control and model groups (P<0.05), whereas serum IL-2 and IL-12 levels were significantly decreased in the cyclophosphamide treatment group compared with the normal, control and model groups (P<0.05). No significant difference in serum levels of tumour necrosis factor-α level was observed between any groups. In S 180 and U 14 solid tumour-bearing mice, no significant differences in serum levels of interferon (INF)-γ level in were observed between groups; however, in H 22 solid tumour-bearing mice, treatment with ASPS significantly increased serum INF-γ compared with the positive control group (P<0.05). The results may provide a basis for the potential application of ASPS in clinical treatment for cancer.

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Inhibition of inducible nitric oxide synthase by Acanthopanax senticosus extract in RAW264.7 macrophages

Cited by 43 publications

References 32 publications

Anti-inflammatory effect of patchouli alcohol isolated from Pogostemonis Herba in LPS-stimulated RAW264.7 macrophages

Anti-inflammatory effect of patchouli alcohol isolated from Pogostemonis Herba in LPS-stimulated RAW264.7 macrophages

Effects of forsythoside on lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages

Anti‑tumour effects of polysaccharide extracted from Acanthopanaxï¿½senticosus and cell‑mediated immunity

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