Anti‑tumour effects of polysaccharide extracted from Acanthopanax�senticosus and cell‑mediated immunity

Abstract

Abstract. Acanthopanax senticosus, also known as Siberian ginseng, is widely distributed throughout northern Asia and used in traditional Chinese medicine; it has been reported to prevent a number of diseases. However, the association between the antitumour and immunostimulatory activities of polysaccharide extracted from A. senticosus (ASPS) remains to be elucidated. The aim of the present study was to investigate the anti-tumour and immunomodulatory effects of polysaccharide extracted from ASPS on Crocker sarcoma S 180 , hepatic carcinoma H 22 and uterine cervical carcinoma U 14 tumour cell lines implanted in mice. High performance liquid chromatography, gas chromatography and infrared spectroscopy were used to analyse the monosaccharide composition of ASPS. The monosaccharide composition of ASPS (Arabic candy: Xylose: Glucose: Mannose) was 7.1:22.3:7.6:1.0. On day 0, female Kunming mice, were injected subcutaneously with 1x10 8 tumour cells in 0.2 ml. The inoculated mice were subsequently divided into five groups (10 mice/group) as follows: Model group, treated with normal saline; positive control group, treated with 30 mg/kg cyclophosphamide (CTX); and three treatment groups, treated with 200, 100 or 50 mg/kg ASPS. Non-inoculated mice were divided into the normal group, which was treated with normal saline, and the negative control group, which was treated with 200 mg/kg ASPS (n=10/group). CTX and ASPS were administered intragastrically once daily for 10 days. All mice were sacrificed on day 11. ASPS was observed to have an inhibitory effect on the growth of S 180 , H 22 and U 14 cells in solid and ascites tumour-bearing mice. Serum interleukin (IL)-2 and IL-12 levels were significantly increased in S 180 solid tumour-bearing mice treated with 200 or 100 mg/kg ASPS compared with mice in the normal, control and model groups (P<0.05), whereas serum IL-2 and IL-12 levels were significantly decreased in the cyclophosphamide treatment group compared with the normal, control and model groups (P<0.05). No significant difference in serum levels of tumour necrosis factor-α level was observed between any groups. In S 180 and U 14 solid tumour-bearing mice, no significant differences in serum levels of interferon (INF)-γ level in were observed between groups; however, in H 22 solid tumour-bearing mice, treatment with ASPS significantly increased serum INF-γ compared with the positive control group (P<0.05). The results may provide a basis for the potential application of ASPS in clinical treatment for cancer.

Efficacy and Safety of Aidi Injection Combined with Transcatheter Arterial Chemoembolization on Primary Hepatic Carcinoma: A Systematic Review and Meta‐Analysis

“…For example, pharmacology study has shown that ADI contained a variety of polysaccharides including astragalus and acanthopanax senticosus which is refined and extracted by modern scientific methods. This can improve the phagocytosis of reticuloendothelial system, stimulate the production of TNF- α (tumor necrosis factor), and enhance the activity of T cells, NK cells, and Lak (lymphoid activated killer cells) of PHCC patients [ 35 – 37 ]. Furthermore, some researchers found that Ginseng (Rensheng) contains Rg3 and RH2 in Ginsenoside which can also enhance and improve the function of T cell and B cell, increase a number of interferon and interleukin, and enhance the killing ability of NK (natural killer) cells and lymphatic factor [ 38 ].…”

Aidi injection as adjunctive treatment to gemcitabine-based chemotherapy for advanced non-small cell lung cancer: a systematic review and meta-analysis

“… 2017 ; Meng et al. 2017 ). Specifically, the following are the pharmacological effects of the active ingredients: (1) the sensitivity of hypoxic lung cancer cells can be increased, cisplatin resistance can be attenuated, and the immune response can be resumed by ginsenoside Rg3; (2) lung cancer cells usually migrate and invade in the body, which can be impaired by cantharidin ; (3) according to the activation of macrophages and natural killer cells, the polysaccharides extracted from Acanthopanax senticosus can inhibit tumour metastasis and play an antitumor role; (4) astragaloside IV can increase the sensitivity of NSCLC cells to gefitinib; (5) under the action of ginseng, the expression of apoptosis protein is increased and that of anti-apoptotic protein is reduced (Yoon et al.…”

Phytoadaptogens in the Tumours Biotherapy and Geriatrics (Part 2)

“…При раке желудка и молочной железы у больных лучевая терапия на фоне высоких доз экстракта элеутерококка в меньшей степени подавляла иммунный статус и число лейкоцитов крови. Показано, что экстракты женьшеня, его полисахариды и гинзенозиды -протопанаксатриолы, панаксатриолы, а также Rh1, Rh20, R-Rh1, F1 -стимулируют продукцию оксида азота, интерлейкинов 4, 12, ИФН-γ иммунокомпетентными клетками [2,32,33].…”