Anti-inflammatory effect of patchouli alcohol isolated from Pogostemonis Herba in LPS-stimulated RAW264.7 macrophages

Abstract

Abstract. pogostemonis herba has long been used in traditional Chinese medicine for the treatment of inflammationrelated disorders. patchouli alcohol (pa) isolated from pogostemonis herba is a tricyclic sesquiterpene that is known to exert a variety of pharmacological activities. the present study aimed to investigate the anti-inflammatory effect of pa on lipopolysaccharide (lpS)-stimulated raW264.7 cells. pre-treatment with pa at concentrations of 10, 20 or 40 µm dose-dependently decreased the production of tumor necrosis factor (tnF)-α, interleukin (il)-1β, il-6, nitric oxide (nO) and prostaglandin E 2 in lpS-stimulated raW264.7 cells. in addition, pa treatment also reversed the increased mrna expression of tnF-α, il-1β, il-6, inducible nitric oxide synthase (inOS) and cyclooxygenase (cOx)-2 caused by lpS in raW264.7 cells. these results indicate that pa is an important anti-inflammatory constituent of Pogostemonis Herba and that its anti-inflammatory effect may be mediated, at least in part, by down-regulation of the mrna expression of a panel of inflammatory mediators, such as TNF-α, il-1β, il-6, inOS and cOx-2.

Immunomodulatory Potential of Patchouli Alcohol Isolated from <i>Pogostemon cablin</i> (Blanco) Benth (Lamiaceae) in Mice

“…PA was isolated as previously described [13]. Briefly, Pogostemon cablin (18 kg) was refluxed with 95% v/v ethanol/aqueous (40 L × 2, 60 min each time).…”

“…It has some interesting pharmacological activities, such as anti-emetic, and anti-influenza virus potency [11,12]. Recent work has also demonstrated the effectiveness of PA as an antiinflammatory agent in vivo and in vitro [10,13].…”

Effects of forsythoside on lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages

“…Macrophages are important immune cells which are the first line of host defense against bacterial infection and cancer growth, and play essential roles in the initiation, maintenance and resolution of inflammation (Yan et al, 2011). When stimulated by lipopolysaccharide (LPS), a bacterial endotoxin, macrophages produce a number of inflammation-related enzymes, cytokines and chemokines, such as tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-6, prostaglandin E2 and nitric oxide (NO), cyclooxygenase-2 and inducible nitric oxide synthase for *Corresponding author.…”

Molecular Dynamic Screening Sesquiterpenoid Pogostemon Herba as Suggested Cyclooxygenase Inhibitor

“…In-vitro analysis of alpha-patchouli alcohol had increase protection against influenza virus infection in mice by increasing the immune response, and attenuation of the systemic inflammatory response (5). Invivo analysis of alpha-patchouli alcohol also had the effect of anti-inflammatory activity, by regulating the mRNA expression of the panel of inflammatory mediators, including TNF-α, IL-1β, iNOS and COX-2 (6). In-vivo analysis of alpha-bulnesene had the ability as an anti-platelet aggregation in rabbit blood by inhibiting the COX enzymes and the mechanism of PAF (Platelet Factor Activating) (7,8).…”