Influence of biflorin on the labelling of red blood cells, plasma protein, cell protein, and lymphocytes with technetium-99m: in vitro study

Abstract

RESUMO: "Infl uência da bifl orina na marcação do tecnécio-99m em células vermelhas do sangue, proteínas do plasma, proteínas celulares e em linfócitos: estudos in vitro". Neste artigo relatam-se os resultados de um estudo in vitro envolvendo a infl uência da bifl orina (uma o-quinona isolada de Capraria bifl ora L. que possui uma potente atividade antimicrobiana) na marcação do Tc-99m em células vermelhas do sangue, proteínas do plasma, proteínas celulares e em linfócitos. O sangue foi coletado de ratos Wistar e incubado com várias concentrações de bifl orina, e soluções de cloreto estanoso (SnCl 2 ) adicionando-se Tc-99m. O plasma (P) e as células vermelhas do sangue (CVS) foram isolados, precipitados e centrifugados, isolando-se as frações solúveis (FS) e insolúveis (FI). A maior concentração de bifl orina (100%) é capaz de reduzir a captação do Tc-99m (%ATI) nas CVS e a fi xação na FI-P. Uma solução de 0,2 mL de linfócitos (2,5 mL; 1.0 x 10 6 células/mL), obtidos por centrifugação de sangue humano tratado com Ficoll-Hypac, foi incubada com bifl orina (0,1 mL). Soluções de cloreto estanoso e Tc-99m foram então adicionadas. Os linfócitos foram separados e o %ATI presente nessas células foi avaliado. Uma redução no %ATI (de 97,85 ± 0,99 a 88,86 ± 5) foi observada para CVS e para FI-P (73,24 ± 5,51 a 20,72 ± 6,95). Os resultados não mostraram decréscimo no %ATI para os linfócitos com bifl orina.Unitermos: Capraria bifl ora, radiofarmacêuticos, pertecnetato de sódio. ABSTRACT:In this paper we report the results of an in vitro study involving the infl uence of bifl orin (an o-quinone isolated from Capraria bifl ora L. that has potent antimicrobial activity) on the Tc-99m labeling of red blood cells, plasma protein, cells protein, and lymphocytes. Blood was withdrawn from Wistar rats and incubated with various concentrations of bifl orin, and solutions of stannous chloride and Tc-99m were added. Plasma (P) and red blood cells (RBC) were isolated, precipitated, and centrifuged, and soluble (SF) and insoluble (IF) fractions were isolated. The results show that the highest concentration (100%) of bifl orin is able to reduce the uptake of Tc-99m (%ATI) on RBC and the fi xation on IF-P. To study the infl uence of bifl orin on 99mTc lymphocyte labeling, human blood was submitted to a technique with Ficoll-Hypac and centrifuged, and white cells were isolated. Lymphocytes (2.5 mL; 1.0 x 10 6 cells/mL) were obtained and a 0.2 mL solution was incubated with bifl orin (0.1 mL). Solutions of stannous chloride and 99mTc were added. Lymphocytes were separated and the %ATI bound in these cells was evaluated. A reduction in %ATI (from 97.85 ± 0.99 to 88.86 ± 5) was observed for RBC and for IF-P (73.24 ± 5.51 to 20.72 ± 6.95). In this case the results showed no decrease in %ATI for the lymphocytes with bifl orin.

Assessment of effects of a formula used in the traditional Chinese medicine (Buzhong Yi Qi Wan) on the morphologic and osmotic fragility of red blood cells

“…Qualitative and quantitative morphological analysis have permitted to verify the effects of natural products on membrane of red blood cells (Oliveira et al, 2002;Moreno et al, 2002;Costa et al, 2002;Oliveira et al, 2003;Fernandes et al, 2005;Aquino et al, 2007). The morphological analysis of blood smears suggested no alteration on shape red blood cells from blood treated with BYQW extract in quantitative and qualitative assays (Figures 1, 2 and 3).…”

Effects of a tomato (Solanum lycopersicum) extract on the labeling of blood constituents with technetium-99m

“…Yet, the tomato extract has not modifi ed the distribution of radioactivity between plasma and blood cells compartments (Figure 2) neither the fi xation of 99m Tc on the blood cells proteins ( Figure 4). Stannous ion (Sn +2 ) is used as reducing agent in the 99m Tc-labeling of blood constituents and compounds or conditions that interfere with its action can alter the fi xation of 99m Tc on these constituents (Hladik III et al, 1987;Bernardo-Filho et al, 1994;Moreno et al, 2002;Fernandes et al, 2005;Aquino et al, 2007). The effect of tomato extract on labeling of plasma proteins could be related to its antioxidant property disturbing the action of Sn +2 on 99m Tc and decreasing the radioactivity uptake by plasma proteins.…”

Assessment of the effect of Bacopa monnieri (L.) Wettst. extract on the labeling of blood elements with technetium-99m and on the morphology of red blood cells

“…In Nuclear medicine technetium-99m (99mTc) has been the most utilized radionuclide both in diagnosis nuclear medicine procedures and in basic scientific research (Srivastava, 1987;Early & Sodee, 1999;Saha, 2003;Hladik et al, 1987;Gutfilen et al, 1993;BernardoFilho et al, 1994aBernardoFilho et al, , 1994bAquino et al, 2007;Paoli et al, 2008;Mousinho et al, 2009). This wide use in nuclear medicine is due to its optimal physical characteristics, convenient availability from Mo-99/Tc-99m generator and negligible environmental impact (Hladik et al, 1987;Early et al, 1999;Saha et al, 2003).…”