This study shows a protective effect or DNA repair mechanism induction by pre-exposure to low intensity red laser on the lethal action of oxidant agents and, therefore, laser therapy protocol should consider fluencies, wavelength and tissue conditions before beginning treatment.

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Background:The ability to control skin blood flow decreases with advancing age and some clinical disorders, as in diabetes and in rheumatologic diseases. Feasible clinical strategies such as whole-body vibration exercise (WBVE) are being used without a clear understanding of its effects. The aim of the present study is to review the effects of the WBVE on blood flow kinetics and its feasibility in different populations.Material and Methods:The level of evidence (LE) of selected papers in PubMed and/or PEDRo databases was determined. We selected randomized, controlled trials in English to be evaluated.Results:Six studies had LE II, one had LE III-2 and one III-3 according to the NHMRC. A great variability among the protocols was observed but also in the assessment devices; therefore, more research about this topic is warranted.Conclusion:Despite the limitations, it is can be concluded that the use of WBVE has proven to be a safe and useful strategy to improve blood flow. However, more studies with greater methodological quality are needed to clearly define the more suitable protocols.

RESUMO: "Efeitos de um extrato de tomate (Solanum lycopersicum) na marcação de constituintes sangüíneos com tecnécio-99m". O tomate (Solanum lycopersicum) é o segundo vegetal mais produzido e consumido no mundo, tendo sido indicado para prevenção e tratamento de câncer, asma e arteriosclerose. Constituintes sangüíneos marcados com radionuclídeos têm sido usados em procedimentos na medicina nuclear. Dados têm mostrado que alimentos e drogas podem alterar a marcação de constituintes sangüíneos com tecnécio-99m ( 99m Tc). Este estudo avaliou a infl uência de um extrato de tomate neste procedimento de radiomarcação. Sangue heparinizado (Wistar rats) foi incubado in vitro com diferentes concentrações de um extrato de tomate e a marcação com 99m Tc foi realizada. Plasma (P) e células sangüíneas (CS) foram separadas permitindo o isolamento das frações solúvel (SF-P/SF-CS) e insolúvel (IF-P/IF-CS) por precipitação e centrifugação. A radioatividade nos constituintes sangüíneos (P, CS, IF-P, SF-P, IF-CS e SF-CS) foi determinada e a porcentagem de radioatividade (%ATI), calculada. O extrato de tomate usado, nas maiores concentrações (2,00 e 4,00g/mL), reduziu signifi cativamente (p < 0,05) a %ATI na IF-P, embora este extrato não tenha modifi cado a radiomarcação da CS e fi xação da radioatividade na IF-CS. Em conclusão, nossos dados sugerem que os compostos químicos presentes no extrato aquoso de tomate teriam algumas propriedades capazes de alterar a fi xação do 99m Tc nas proteínas plasmáticas.Unitermos: Solanum lycopersicum, Solanaceae, constituintes sangüíneos, tecnécio-99m.ABSTRACT: Tomato (Solanum lycopersicum) is the second most produced and consumed vegetable in the world. It has been indicated in the prevention and treatment of cancer, asthma and atherosclerosis. Blood constituents labeled with radionuclides have been used in procedures in nuclear medicine. Data have shown that food and drugs can alter the labeling of blood constituents with technetium-99m ( 99m Tc). This study evaluated the infl uence of a tomato extract on this radiolabeling procedure. Heparinized blood (Wistar rats) was incubated in vitro with different concentrations of a tomato extract and 99m Tc-labeling was performed. Plasma (P) and blood cells (BC) were separated following soluble (SF-P/SF-BC) and insoluble (IF-P/IF-BC) fractions isolation by precipitation and centrifugation. The radioactivities on blood constituents (P, BC, IF-P, SF-P, IF-BC and SF-BC) were determined and the percentage of radioactivity (%ATI) was calculated. The tomato extract used at the highest concentrations (2.00 and 4.00g/ mL), reduced signifi cantly (p < 0.05) the %ATI in IF-P, although this extract did not modify the radiolabeling on BC, neither the radioactivity fi xation on IF-BC. In conclusion, our data suggest that the chemical compounds present in the aqueous tomato extract could have some properties capable of change the fi xation of 99m Tc on plasma proteins.

Clove (Caryophyllus aromaticus L.) has been used for clinical procedures. Blood constituents labeled with

RESUMO: "Efeitos osmótico e morfológico na membrana de hemácias: ação de um extrato aquoso de Lantana camara". As infusões de folhas de Lantana camara (cambara-de-espinho) são usadas popularmente em alguns países para tratar doenças gastrointestinais. O experimento de fragilidade osmótica e a análise morfométrica têm sido usados para verifi car a interação de drogas com a membrana de hemácias. O objetivo deste trabalho foi avaliar os efeitos de um extrato aquoso de Lantana camara na fragilidade osmótica e na morfologia de hemácias. Amostras de sangue foram tratadas com extrato de Lantana camara (10 mg/mL), o ensaio de fragilidade osmótica e a análise morfológica foram realizadas. Na presença do extrato, os dados obtidos indicaram (i) um aumento signifi cativo (p < 0,05) da hemólise e (ii) modifi cações na morfologia das hemácias. Estes efeitos da Lantana camara poderiam estar associados com algumas propriedades farmacológicas de compostos químicos do extrato estudado.Unitermos: Lantana camara, Verbenaceae, morfologia, fragilidade osmótica, hemácias. ABSTRACT:The Lantana camara ("cambara de espinho") leaves infusions are used popularly in some countries to treat gastrointestinal diseases. Osmotic fragility assay and morphometric analysis have been used to verify the interaction of drugs with the membrane of red blood cells (RBC). The aim of this work was to evaluate the effects of an aqueous extract of Lantana camara on the osmotic fragility and on the morphology of RBC. Blood samples were treated with extract of Lantana camara (10 mg/mL), osmotic fragility assay and morphological analysis were carried out. In the presence of the extract, the data obtained indicated (i) a signifi cant (p < 0.05) increase of hemolysis and (ii) modifi cations on the morphology of RBC. These effects of the Lantana camara may be associated with some pharmacological properties of the chemical compounds of this studied extract.

Mentha crispa extract or 0.9% NaCl were (15 days) administered daily by gavage to Wistar rats. Animals then received Na 99m TcO 4 via ocular plexus and were sacrificed. Organs were isolated, radioactivity was determined, percentages of radioactivity in each organ was calculated, and statistical analyses performed. Significant (p = 0.0061) increases of radioactivity were observed in kidney, spleen, and thyroid, which could be exampled by the effects of the extract and/or by the generation of active metabolites.

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