Laser for treatment of aphthous ulcers on bacteria cultures and DNA

Marciano, Roberta da Silva; Sergio, Luiz Philippe da Silva; Polignano, Giovanni Augusto Castanheira; Presta, G.; Guimarães, Oscar Roberto; Geller, Mauro; Paoli, Severo de; Paoli, Flávia de; Fonseca, Adenilson de Souza da

doi:10.1039/c2pp25027f

Cited by 27 publications

(47 citation statements)

References 48 publications

(69 reference statements)

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“…Due to these properties, T 4 endonuclease V protects patients with xeroderma pigmentosum against skin cancer ( 32 ). Plasmid DNA exposed to low-intensity red and infrared lasers did not show any alterations in electrophoretic profile in agarose gels, suggesting absence of detectable single- or double-strand DNA breaks, as previous studies have demonstrated ( 19 , 20 ). In addition, the plasmids did not show any alteration in their electrophoretic profile after incubation with T 4 endonuclease V, suggesting that red and infrared laser lights did not induce DNA lesions targeted by this enzyme, at least under the laser irradiation conditions used in this study.…”

Section: Methodssupporting

confidence: 80%

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Nucleotide excision repair pathway assessment in DNA exposed to low-intensity red and infrared lasers

Fonseca

Campos²,

Magalhães³

et al. 2015

Braz J Med Biol Res

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Low-intensity lasers are used for prevention and management of oral mucositis induced by anticancer therapy, but the effectiveness of treatment depends on the genetic characteristics of affected cells. This study evaluated the survival and induction of filamentation of Escherichia coli cells deficient in the nucleotide excision repair pathway, and the action of T4endonuclease V on plasmid DNA exposed to low-intensity red and near-infrared laser light. Cultures of wild-type (strain AB1157) E. coli and strain AB1886 (deficient in uvrA protein) were exposed to red (660 nm) and infrared (808 nm) lasers at various fluences, powers and emission modes to study bacterial survival and filamentation. Also, plasmid DNA was exposed to laser light to study DNA lesions produced in vitro by T4endonuclease V. Low-intensity lasers:i) had no effect on survival of wild-type E. coli but decreased the survival of uvrA protein-deficient cells,ii) induced bacterial filamentation, iii) did not alter the electrophoretic profile of plasmids in agarose gels, andiv) did not alter the electrophoretic profile of plasmids incubated with T4 endonuclease V. These results increase our understanding of the effects of laser light on cells with various genetic characteristics, such as xeroderma pigmentosum cells deficient in nucleotide excision pathway activity in patients with mucositis treated by low-intensity lasers.

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Section: Methodssupporting

confidence: 80%

“…Previous studies have demonstrated induction of filamentation in E. coli cells exposed to low-intensity lasers ( 1820 ). Wild-type E. coli cells (strain AB1157) exhibited a filamentous phenotype after exposure to both red and infrared lasers, and primarily with infrared lasers at the highest fluence (100 J/cm 2 ).…”

Section: Methodsmentioning

confidence: 99%

Nucleotide excision repair pathway assessment in DNA exposed to low-intensity red and infrared lasers

Fonseca

Campos²,

Magalhães³

et al. 2015

Braz J Med Biol Res

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“…Induction of filamentation phenotype is reported in E. coli cells irradiated with the low-level lasers, as in those with mutations in genes related to DNA repair pathways for oxidative damages. 15,16,20 However, the filamentation assay performed in these studies did not allow for evaluating what happened with cells which do not present this phenotype. To attempt this need, area and perimeter were measured in mutant E. coli cells in SOS functions (AB2463 and AB2494) and compared to E. coli wild type cells (AB1157).…”

Section: Discussionmentioning

confidence: 99%

“…12,14 In addition, these lasers are capable of inducing a filamentation phenotype, one of SOS responses, induced in some bacterial species exposed to harmful environments. 15,16 Despite so, low-level laser therapies are used for treatment of diseases, particularly oral cavity diseases induced by chemotherapy and radiotherapy in oncology. The aim of this work was assessing the effects of low power lasers on mutant Escherichia coli cells in DNA repair.…”

Section: Introductionmentioning

confidence: 99%

Low-Power Red and Infrared Laser Effects on Cells Deficient in DNA Repair

2019

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Introduction: Low-level lasers are successfully used to prevent and treat diseases in soft oral and bone tissues, particularly diseases in oral cavity caused by chemotherapy and radiotherapy in oncology. However, controversy exists as to whether these lasers induce molecular side effects, mainly on DNA. The aim of this work was to assess the effects of low-power lasers on mutant Escherichia coli cells in DNA repair. Methods: Escherichia coli wild type cultures as well as those lacking recombination DNA repair (recA- ) and la SOS responses (lexA- ) irradiated with lasers at different energy densities, powers, and emission modes for cell viability and morphology assessment were used in this study. Results: Laser irradiation: (i) did not affect cell viability of non-mutant and lexA- cells but decreased viability in recA- cultures; (ii) altered morphology of wild type and lexA, depending on the energy density, power, emission mode, and wavelength. Conclusion: Results show that low-level lasers have lethal effects on both recombination DNA repair and SOS response bacterial cells but do not induce morphological modifications in these cells.

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“…NER acts on non-specific oxidative lesions while BER acts on specific lesions induced by DNA molecule reaction with oxidative agents, such as free radicals [12,37]. Red and infrared laser radiations at low fluences have been suggested as producing free radicals with potential lesion induction in DNA molecule [37,38].…”

Section: Discussionmentioning

confidence: 99%

Low-intensity red and infrared lasers affect mRNA expression of DNA nucleotide excision repair in skin and muscle tissue

et al. 2016

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Lasers emit light beams with specific characteristics, in which wavelength, frequency, power, fluence, and emission mode properties determine the photophysical, photochemical, and photobiological responses. Low-intensity lasers could induce free radical generation in biological tissues and cause alterations in macromolecules, such as DNA. Thus, the aim of this work was to evaluate excision repair cross-complementing group 1 (ERCC1) and excision repair cross-complementing group 2 (ERCC2) messenger RNA (mRNA) expression in biological tissues exposed to low-intensity lasers. Wistar rat (n = 28, 4 for each group) skin and muscle were exposed to low-intensity red (660 nm) and near-infrared (880 nm) lasers at different fluences (25, 50, and 100 J/cm(2)), and samples of these tissues were withdrawn for RNA extraction, cDNA synthesis, and gene expression evaluation by quantitative polymerase chain reaction. Laser exposure was in continuous wave and power of 100 mW. Data show that ERCC1 and ERCC2 mRNA expressions decrease in skin (p < 0.001) exposed to near-infrared laser, but increase in muscle tissue (p < 0.001). ERCC1 mRNA expression does not alter (p > 0.05), but ERCC2 mRNA expression decreases in skin (p < 0.001) and increases in muscle tissue (p < 0.001) exposed to red laser. Our results show that ERCC1 and ERCC2 mRNA expression is differently altered in skin and muscle tissue exposed to low-intensity lasers depending on wavelengths and fluences used in therapeutic protocols.

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Laser for treatment of aphthous ulcers on bacteria cultures and DNA

Cited by 27 publications

References 48 publications

Nucleotide excision repair pathway assessment in DNA exposed to low-intensity red and infrared lasers

Nucleotide excision repair pathway assessment in DNA exposed to low-intensity red and infrared lasers

Low-Power Red and Infrared Laser Effects on Cells Deficient in DNA Repair

Low-intensity red and infrared lasers affect mRNA expression of DNA nucleotide excision repair in skin and muscle tissue

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