Individuell mit CAD-CAM-Technik hergestellte, bioresorbierbare dreidimensionale Polycaprolaktongerüste zur experimentellen Rekonstruktion von kraniofazialen Defekten beim Schwein

Rohner, Dennis; Hutmacher, Dietmar W.; See, Patrick; Tan, Kim Cheng; Yeow, Vincent; Sb, Tan; Lee, S.; Hammer, Beat

doi:10.1007/s10006-002-0389-0

Cited by 25 publications

(9 citation statements)

References 17 publications

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“…Its use could be translated from bench to clinical use easily due to the approval status by the U.S. Food and Drug Administration (FDA). For bone TE, scaffolds made from PCL have been shown previously to support ectopic bone formation in a rat model [16], repair orbital defects in a pig model [17], and burr hole defects in patients with chronic subdural hematoma [18]. …”

Section: Introductionmentioning

confidence: 99%

Bone Tissue Engineering with Adipose-Derived Stem Cells in Bioactive Composites of Laser-Sintered Porous Polycaprolactone Scaffolds and Platelet-Rich Plasma

2013

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Three-dimensional porous polycaprolactone (PCL) scaffolds with consistent inter-pore channels, 83% porosity and 300–400 μm pore size were fabricated via selective laser sintering. The PCL scaffold was combined with platelet-rich plasma (PRP) to form a bioactive composite and studied for potential application in bone tissue engineering using porcine adipose-derived stem cells (PASCs). The PCL/PRP/PASCs construct showed enhanced cell seeding efficiency and synergistically increased the differentiation capability of PASCs in osteogenic medium toward the osteoblast lineage, judging from elevated alkaline phosphatase activity and up-regulated osteogenic genes expression. For in vivo study, a 3 cm × 3 cm mandible defect was created in pigs and reconstructed by implanting acellular PCL scaffolds or PCL/PRP/PASCs constructs. Both groups showed new bone formation, however, the new bone volume was 5.1 times higher for PCL/PRP/PASCs 6 months post-operation. The bone density was less and loose in the acellular PCL group and the Young’s modulus was only 29% of normal bone. In contrast, continued and compact bone formation was found in PCL/PRP/PASCs and the Young’s modulus was 81% that of normal bone. Masson’s trichrome stain, immunohistochemical analysis of osteocalcin and collagen type I also confirmed new bone formation.

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Section: Introductionmentioning

confidence: 99%

Bone Tissue Engineering with Adipose-Derived Stem Cells in Bioactive Composites of Laser-Sintered Porous Polycaprolactone Scaffolds and Platelet-Rich Plasma

2013

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“…Therapy concepts based on application of adult stem cells were thought to be promising for complete regeneration of breast tissue in the early days in the field; however, those concepts also have considerable disadvantages impeding their clinical translation–ranging from complexities and cost with scaling up of tissue culture to requiring complex GMP-certified laboratories for the processing12131415161718. Furthermore, it is challenging to efficiently vascularise large clinically relevant breast scaffolds (>75 cc) using cell culture-based concepts.…”

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confidence: 99%

Transformation of Breast Reconstruction via Additive Biomanufacturing

Chhaya

Balmayor

Hutmacher

et al. 2016

Sci Rep

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Adipose tissue engineering offers a promising alternative to current breast reconstruction options. However, the conventional approach of using a scaffold in combination with adipose-derived precursor cells poses several problems in terms of scalability and hence clinical feasibility. Following the body-as-a-bioreactor approach, this study proposes a unique concept of delayed fat injection into an additive biomanufactured and custom-made scaffold. Three study groups were evaluated: Empty scaffold, Scaffold containing 4 cm3 lipoaspirate and Empty scaffold +2-week prevascularisation period. In group 3, of prevascularisation, 4 cm3 of lipoaspirate was injected into scaffolds after 2 weeks. Using a well-characterised additive biomanufacturing technology platform, patient-specific scaffolds made of medical-grade-polycaprolactone were designed and fabricated. Scaffolds were implanted in subglandular pockets in immunocompetent minipigs (n = 4) for 24-weeks. Angiogenesis and adipose tissue regeneration were observed in all constructs. Histological evaluation showed that the prevascularisation + lipoaspirate group had the highest relative area of adipose tissue (47.32% ± 4.12) which was significantly higher than both lipoaspirate-only (39.67% ± 2.04) and empty control group (8.31% ± 8.94) and similar to native breast tissue (44.97% ± 14.12). This large preclinical animal study provides proof-of-principle that the clinically applicable prevascularisation and delayed fat-injection techniques can be used for regeneration of large volumes of adipose tissue.

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“…In this case, SLM technology reduces the density of pure stainless steel from 7.83 per cm 3 to 0.6 g/cm 3 . Nearly 90% reduction in weight but still retaining enormous strength and stiffness with material properties the same as with rolled steel.…”

Section: The Selective Laser Melting Process (Slm)mentioning

confidence: 96%

“…Ultra light mesh structures are some of the most complex and dimensionally demanding geometries ever generated in metals, with over 450 holes and channels per cm 3 . In this case, SLM technology reduces the density of pure stainless steel from 7.83 per cm 3 to 0.6 g/cm 3 .…”

Section: The Selective Laser Melting Process (Slm)mentioning

confidence: 99%

“…In the meantime, rapid prototyping processes have been developed further for plastic and metal materials to become Rapid Manufacturing (RM) Systems. This creates an exciting basis for the use of biocompatible materials and even tissue engineering in individual, pre-determined, threedimensional replacement of bony structures in clinical applications [2,3].…”

Section: Introductionmentioning

confidence: 99%

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Implant design and production—a new approach by selective laser melting

Wehmöller

Warnke

Zilian³

et al. 2005

International Congress Series

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Individuell mit CAD-CAM-Technik hergestellte, bioresorbierbare dreidimensionale Polycaprolaktongerüste zur experimentellen Rekonstruktion von kraniofazialen Defekten beim Schwein

Abstract: The PCL scaffold coated with bone marrow seems to be a material that effectively provides osteoinduction with formation of new bone. Long-term results at 12 months are still pending.

Cited by 25 publications

References 17 publications

Bone Tissue Engineering with Adipose-Derived Stem Cells in Bioactive Composites of Laser-Sintered Porous Polycaprolactone Scaffolds and Platelet-Rich Plasma

Bone Tissue Engineering with Adipose-Derived Stem Cells in Bioactive Composites of Laser-Sintered Porous Polycaprolactone Scaffolds and Platelet-Rich Plasma

Transformation of Breast Reconstruction via Additive Biomanufacturing

Implant design and production—a new approach by selective laser melting

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