Selective laser melting (SLM), a method used in the nuclear, space, and racing industries, allows the creation of customized titanium alloy scaffolds with highly defined external shape and internal structure using rapid prototyping as supporting external structures within which bone tissue can grow. Human osteoblasts were cultured on SLM-produced Ti6Al4V mesh scaffolds to demonstrate biocompatibility using scanning electron microscopy (SEM), fluorescence microscopy after cell vitality staining, and common biocompatibility tests (lactate dihydrogenase (LDH), 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT), 5-bromo-2-deoxyuridine (BrdU), and water soluble tetrazolium (WST)). Cell occlusion of pores of different widths (0.45-1.2 mm) was evaluated. Scaffolds were tested for resistance to compressive force. SEM investigations showed osteoblasts with well-spread morphology and multiple contact points. Cell vitality staining and biocompatibility tests confirmed osteoblast vitality and proliferation on the scaffolds. Pore overgrowth increased during 6 weeks' culture at pore widths of 0.45 and 0.5 mm, and in the course of 3 weeks for pore widths of 0.55, 0.6, and 0.7 mm. No pore occlusion was observed on pores of width 0.9-1.2 mm. Porosity and maximum compressive load at failure increased and decreased with increasing pore width, respectively. In summary, the scaffolds are biocompatible, and pore width influences pore overgrowth, resistance to compressive force, and porosity.
PRF appears to be superior to collagen (Bio-Gide) as a scaffold for human periosteal cell proliferation. PRF membranes are suitable for in vitro cultivation of periosteal cells for bone tissue engineering.
There
is an urgent need for ultrarapid testing regimens to detect
the severe acute respiratory syndrome coronavirus 2 [SARS-CoV-2] infections
in real-time within seconds to stop its spread. Current testing approaches
for this RNA virus focus primarily on diagnosis by RT-qPCR, which
is time-consuming, costly, often inaccurate, and impractical for general
population rollout due to the need for laboratory processing. The
latency until the test result arrives with the patient has led to
further virus spread. Furthermore, latest antigen rapid tests still
require 15–30 min processing time and are challenging to handle.
Despite increased polymerase chain reaction (PCR)-test and antigen-test
efforts, the pandemic continues to evolve worldwide. Herein, we developed
a superfast, reagent-free, and nondestructive approach of attenuated
total reflection Fourier-transform infrared (ATR-FTIR) spectroscopy
with subsequent chemometric analysis toward the prescreening of virus-infected
samples. Contrived saliva samples spiked with inactivated γ-irradiated
COVID-19 virus particles at levels down to 1582 copies/mL generated
infrared (IR) spectra with a good signal-to-noise ratio. Predominant
virus spectral peaks are tentatively associated with nucleic acid
bands, including RNA. At low copy numbers, the presence of a virus
particle was found to be capable of modifying the IR spectral signature
of saliva, again with discriminating wavenumbers primarily associated
with RNA. Discrimination was also achievable following ATR-FTIR spectral
analysis of swabs immersed in saliva variously spiked with virus.
Next, we nested our test system in a clinical setting wherein participants
were recruited to provide demographic details, symptoms, parallel
RT-qPCR testing, and the acquisition of pharyngeal swabs for ATR-FTIR
spectral analysis. Initial categorization of swab samples into negative
versus positive COVID-19 infection was based on symptoms and PCR results
(
n
= 111 negatives and 70 positives). Following training
and validation (using
n
= 61 negatives and 20 positives)
of a genetic algorithm-linear discriminant analysis (GA-LDA) algorithm,
a blind sensitivity of 95% and specificity of 89% was achieved. This
prompt approach generates results within 2 min and is applicable in
areas with increased people traffic that require sudden test results
such as airports, events, or gate controls.
Within the limits of the study, we conclude that for bone defects larger than 4 mm in case of peri-implantitis, this single surgical intervention provided a reliable method to reduce bone defects.
Hydroxyapatite (HAP) and tricalcium phosphate (TCP) are two very common ceramic materials for bone replacement. However, in general HAP and TCP scaffolds are not tailored to the exact dimensions of the defect site and are mainly used as granules or beads. Some scaffolds are available as ordinary blocks, but cannot be customized for individual perfect fit. Using computer-assisted 3D printing, an emerging rapid prototyping technique, individual three-dimensional ceramic scaffolds can be built up from TCP or HAP powder layer by layer with subsequent sintering. These scaffolds have precise dimensions and highly defined and regular internal characteristics such as pore size. External shape and internal characteristics such as pore size can be fabricated using Computer Assisted Design (CAD) based on individual patient data. Thus, these scaffolds could be designed as perfect fit replacements to reconstruct the patient's skeleton. Before their use as bone replacement materials in vivo, in vitro testing of these scaffolds is necessary. In this study, the behavior of human osteoblasts on HAP and TCP scaffolds was investigated. The commonly used bone replacement material BioOss(R) served as control. Biocompatibility was assessed by scanning electron microscopy (SEM), fluorescence microscopy after staining for cell vitality with fluorescin diacetate (FDA) and propidium iodide (PI) and the MTT, LDH, and WST biocompatibility tests. Both versions were colonised by human osteoblasts, however more cells were seen on HAP scaffolds than TCP scaffolds. Cell vitality staining and MTT, LDH, and WST tests showed superior biocompatibility of HAP scaffolds to BioOss, while BioOss was more compatible than TCP. Further experiments are necessary to determine biocompatibility in vivo. Future modifications of 3D printed scaffolds offer advantageous features for Tissue Engineering. The integration of channels could allow for vascular and nerve ingrowth into the scaffold. Also the complex shapes of convex and concave articulating joint surfaces maybe realized with these rapid prototyping techniques.
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