Transformation of Breast Reconstruction via Additive Biomanufacturing

Chhaya, Mohit P.; Balmayor, Elizabeth R.; Hutmacher, Dietmar W.; Schantz, Jan‐Thorsten

doi:10.1038/srep28030

Cited by 77 publications

(88 citation statements)

References 58 publications

(74 reference statements)

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“…In an earlier study, we showed a safe and easy use of tissue engineering-derived surgical implants in maxillofacial surgery [6, 7]. This technology is based on computer-assisted design/computer-assisted manufacturing (CAD/CAM) and polycaprolactone (PCL) implants for the regeneration of bone [8–12] and other tissues [13, 14]. PCL is a biocompatible material and PCL-based implants biodegrade within 2 years and promote autologous bone formation [15].…”

Section: Introductionmentioning

confidence: 99%

In vivo evaluation of a regenerative approach to nasal dorsum augmentation with a polycaprolactone-based implant

et al. 2019

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BackgroundAlternative techniques for nasal dorsum augmentation are of paramount importance in reconstructive and plastic surgery. In contrast to autologous cartilage grafts, tissue-engineered grafts can be created de novo and yield low–none donor site morbidity as compared to autologous grafts like rib or ear cartilage. To address this demand, this study investigated the in vivo regenerative potential of polycaprolactone-based implants as an alternative to autologous cartilage grafting during rhinoplasty.MethodsImplants were placed at the nasal dorsum in two groups of minipigs and kept in situ for 2 and 6 months, respectively. Subsequently, the implants were harvested and examined by histology (hematoxylin–eosin, alcian blue, and safranin O) and immunostaining (collagen I and collagen II). Further analysis was performed to measure diameter and distance of polycaprolactone struts.ResultsHistological examination revealed a persistent formation of connective tissue with some spots resembling a cartilaginous-like matrix after 6 months. In such areas, cells of chondrocyte appearance could be identified. There was a significant decrease in strut diameter but a non-significant difference in strut distance.ConclusionOur results indicated that the investigated polycaprolactone-based implants have shown a regenerative and stable nasal dorsum augmentation after 6 months in vivo. Thus, we believe that customized polycaprolactone-based implants could become an alternative technique for nasal dorsum augmentation without the need for autologous cartilage grafts.

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Section: Introductionmentioning

confidence: 99%

In vivo evaluation of a regenerative approach to nasal dorsum augmentation with a polycaprolactone-based implant

et al. 2019

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“…Indeed, size and shape of the tissues to be regenerated may require personalization to best fit the anatomy of each individual and therefore TECs may need to be customized. In fact, 3D printing medical scaffolds were tailored for breast reconstruction using medical imaging techniques demonstrating the potential interest in the medical field 34 .…”

Section: Discussionmentioning

confidence: 99%

Rationale for the design of 3D-printable bioresorbable tissue-engineering chambers to promote the growth of adipose tissue

Faglin

Gradwohl

Depoortère

et al. 2020

Sci Rep

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Tissue engineering chambers (TECs) bring great hope in regenerative medicine as they allow the growth of adipose tissue for soft tissue reconstruction. To date, a wide range of TEC prototypes are available with different conceptions and volumes. Here, we addressed the influence of TEC design on fat flap growth in vivo as well as the possibility of using bioresorbable polymers for optimum TEC conception. In rats, adipose tissue growth is quicker under perforated TEC printed in polylactic acid than non-perforated ones (growth difference 3 to 5 times greater within 90 days). Histological analysis reveals the presence of viable adipocytes under a moderate (less than 15% of the flap volume) fibrous capsule infiltrated with CD68 + inflammatory cells. CD31-positive vascular cells are more abundant at the peripheral zone than in the central part of the fat flap. Cells in the TEC exhibit a specific metabolic profile of functional adipocytes identified by 1 H-NMR. Regardless of the percentage of TEC porosity, the presence of a flat base allowed the growth of a larger fat volume (p < 0.05) as evidenced by MRI images. In pigs, bioresorbable TEC in poly[1,4-dioxane-2,5-dione] (polyglycolic acid) PURASORB PGS allows fat flap growth up to 75 000 mm 3 at day 90, (corresponding to more than a 140% volume increase) while at the same time the TEC is largely resorbed. No systemic inflammatory response was observed. Histologically, the expansion of adipose tissue resulted mainly from an increase in the number of adipocytes rather than cell hypertrophy. Adipose tissue is surrounded by perfused blood vessels and encased in a thin fibrous connective tissue containing patches of CD163 + inflammatory cells. Our large preclinical evaluation defined the appropriate design for 3D-printable bioresorbable TECs and thus opens perspectives for further clinical applications. Reconstruction of adipose tissue defects after surgery (e.g. mastectomy) or trauma remains a major surgical challenge. Numerous tissue-engineering techniques were attempted experimentally. It includes repair methods such as autologous or heterologous fat grafting or also transfer of vascularized adipose tissue from a donor site, also called a fat flap. The limitation of this latter method is that the fat flap requires a sufficient amount of available fat in the donor site, which often leads to deformity at the donor site. Moreover, donor flap sites may not

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“…In drug-treated group, vessel section tended to be long and narrow, which contrasted with the round shaped vessel section observed in the control group. Histological section has already been used to primitively analysis the vessel shape and orientation 32,33 . In order to quantitatively describe the vessel orientation from histological observation, we introduced a novel statistical magnitude, vessel shape index (VSI), which was calculated by dividing the width of the vessel section by its length.…”

Section: Discussionmentioning

confidence: 99%

The pro-angiogenic role of hypoxia inducible factor stabilizer FG-4592 and its application in an in vivo tissue engineering chamber model

Zhou

Hou

et al. 2019

Sci Rep

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Tissue engineering is a promising technology used as an alternative to organ/tissue transplantation which is often limited by donor shortage. The construction of large-sized engineered tissue requires a fast and sufficient vascularization process. Previous studies have shown that hypoxia-inducible factor (HIF) -1α may promote the vascularization process implying that stabilized HIF-1α can be applied in the engineering of large-sized tissue. However, the toxicity and off-target effect of previously reported HIF-1α stabilizers limit their clinical application. FG-4592, a small molecule specific HIF stabilizer, was previously investigated as an anti-anemia drug in a phase-III clinical trial. Here we found that FG-4592 promoted tube formation in an in vitro model of angiogenesis by stabilizing HIF-1α and activating vascular endothelial growth factor (VEGF). When FG-4592 immobilized fibrin gel scaffold was implanted into a subcutaneous tissue engineering chamber, the vascularization process was significantly enhanced through the similar mechanisms which was verified in vitro . We conclude that FG-4592 may serve as a pro-angiogenic molecule for the construction of large-sized engineered tissue where intensive angiogenesis is required.

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Transformation of Breast Reconstruction via Additive Biomanufacturing

Cited by 77 publications

References 58 publications

In vivo evaluation of a regenerative approach to nasal dorsum augmentation with a polycaprolactone-based implant

In vivo evaluation of a regenerative approach to nasal dorsum augmentation with a polycaprolactone-based implant

Rationale for the design of 3D-printable bioresorbable tissue-engineering chambers to promote the growth of adipose tissue

The pro-angiogenic role of hypoxia inducible factor stabilizer FG-4592 and its application in an in vivo tissue engineering chamber model

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