In Vivo Evaluation of Wound Healing and Anti-Inflammatory Activity of Methanolic Extract of Roots of Centaurea Africana (L.) in Topical Formulation

Abstract

Objective: The present study was to evaluate the anti-inflammatory and wound healing activities of methanolic extract of Centaurea africana rootsin albino Wistar rats.Methods: Following extraction of the C. africana roots with 80% methanol, the extract was formulated as an ointment (5% and 10% w/w). The ointmentwas then evaluated for wound healing activity using excision and incision wound models. Parameters, including wound contraction, epithelizationtime, histopathologically, and hydroxyproline content, were determined using the excision model, whereas tensile strength was measured from theincision model. In parallel, edema of the ear, locally induced by croton oil, was studied for the assessment of anti-inflammatory activity.Results: Wound treated with 5% and 10% methanolic extract ointment exhibited a significant wound healing activity in both models as evidencedby increased wound contraction, shorter epithelization time, higher tissue breaking strength, and increased hydroxyproline content. The methanolicextract also produced dose-related significant reduction (p<0.001) of inflammation.Conclusion: Results of the present study revealed that C. africana displays remarkable wound healing and anti-inflammatory activities.Keywords: Anti-inflammatory activity, Centaurea africana roots, Methanolic extract, Incision and excision wound model.

THE ANTI-INFLAMMATORY ACTIVITY OF ESSENTIAL OIL OF CLOVE (Syzygium aromaticum) IN ABSORPTION BASE OINTMENT WITH ADDITION OF OLEIC ACID AND PROPYLENE GLYCOL AS ENHANCER

“…It means that croton oil can cause irritation and swelling of the skin if it was used Formula I (FI) with composition of 100% oleic acid and 0% propylene glycol Formula II (FII) with composition of 50% oleic acid and 50% propylene glycol Formula III (FIII) with composition of 0% oleic acid and 100% propylene glycol topically [14]. On histochemical observations by using the HE method, crotton oil that was administrated topically can induce hyperplasia, infiltration of leukocytes, edema, neutrophil infiltration, a prostaglandin production and an increase in vascular permeability [15][16][17]. There was a significant difference between negative control and positive control.…”

Current Methods for the Discovery of New Active Ingredients from Natural Products for Cosmeceutical Applications

“…The period of epithelization is determined by the time it takes for the scab to drop from the wound, since it is considered the endpoint of complete epithelization [177]. The wound index is measured using the scoring system described in the study by Zaouani et al [178]. Assessment of hydroxyproline content is used to estimate the amount of collagen, a key marker of wound healing.…”

Anti-Inflammatory Properties of Pavetta Crassicaulis Bremek. Leaf and Flower Crude Extracts and Its Pure Compounds Collected From Western Ghats, Karnataka, India

“…Some of the researchers used different methods of anti-inflammatory activity such as croton oil-induced ear edema in rats [80,81], protein denaturation bioassay [82][83][84][85][86], membrane stabilization assay using human red blood cell [87,88], but most of the researchers followed carrageenan-induced rat paw edema [89][90][91][92][93] to evaluated antiinflammatory activity.…”