Marrubium vulgare and Cytisus triflorus are widely distributed in the Mediterranean area and are used in popular herbal medicine for their various therapeutic effects. The objective of this study is to evaluate their antibacterial, antioxidant and cicatrizing activity in order to verify their traditional use. The total phenol and flavonoid content of the extracts was determined by the Folin–Ciocalteu method and by the AlCl3 method, respectively. The antioxidant activity was evaluated by the DPPH (diphenylpicrylhydrazyl) method. The antimicrobial activity was evaluated by the microdilution method. Moreover, the healing activity was evaluated by the excisional method on rats. The quality and the rate of healing were compared between the test groups (treated with an ointment containing 5% of Marrubium vulgare and Cytisus triflorus extracts) and the control groups (Madecassol® and vehicle). The rate of wound contraction was monitored every three days for 15 days. At the end of the experimental period, cutaneous biopsies were performed for histopathological analysis. The results obtained showed a high content of the extracts in polyphenols and flavonoids, which confer them significant antioxidant and antimicrobial effects. In addition, a clear decrease in the area of the wounds of the experimental groups as compared to the controls was observed as well as an improvement in the quality of the scar tissue.
Objective: The present study was to evaluate the anti-inflammatory and wound healing activities of methanolic extract of Centaurea africana rootsin albino Wistar rats.Methods: Following extraction of the C. africana roots with 80% methanol, the extract was formulated as an ointment (5% and 10% w/w). The ointmentwas then evaluated for wound healing activity using excision and incision wound models. Parameters, including wound contraction, epithelizationtime, histopathologically, and hydroxyproline content, were determined using the excision model, whereas tensile strength was measured from theincision model. In parallel, edema of the ear, locally induced by croton oil, was studied for the assessment of anti-inflammatory activity.Results: Wound treated with 5% and 10% methanolic extract ointment exhibited a significant wound healing activity in both models as evidencedby increased wound contraction, shorter epithelization time, higher tissue breaking strength, and increased hydroxyproline content. The methanolicextract also produced dose-related significant reduction (p<0.001) of inflammation.Conclusion: Results of the present study revealed that C. africana displays remarkable wound healing and anti-inflammatory activities.Keywords: Anti-inflammatory activity, Centaurea africana roots, Methanolic extract, Incision and excision wound model.
Olea europaea var. sylvestris, also named oleaster, is widely used by traditional medicine practitioners in Algeria to treat high blood pressure and diabetes. However, the antidiarrhoeal activity of this plant has not been scientifically evaluated. The main aim of the study deals with an investigation of three topics: the phytochemical screening, the acute toxicity, and antidiarrhoeal activity of the oleaster leaf aqueous extract. Acute oral toxicity study was carried out based on Organization for Economic Cooperation and Development 423 guideline. The extract was orally administered in wistar rats at a single dose of 2000 mg/kg body weight and the animals were observed for mortality, behavioral changes and other abnormal signs. Qualitative analysis of phytochemical constituents was carried out using standard methods developed by Harborne, Trease and Evans. Castor oil-induced diarrhoea tests and gastro intestinal motility assay were evaluated in rats to determine the antidiarrhoeal activity of the extract. In the acute toxicity study, the extract did not induce death or any sign of toxicity in treated rats. The preliminary phytochemical screening of the extract revealed the presence of saponins, flavonoids, and triterpenoids. The oleaster extract at oral doses of 100, 200 and 400 mg/kg body weight showed a significant (P<0.05) antidiarrhoeal activity compared to the control group treated with castor oil induced diarrhoea, enteropooling and gastrointestinal motility assay, after charcoal meal administration. The oleaster leaf aqueous extract has shown a gradual response with increasing dose. The present study indicates that the oleaster leaf aqueous extract is safe with antidiarrhoeal property.
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