In vitro bioactivity and osteoblast-like cell test of zinc containing fluoridated hydroxyapatite films

Abstract

Zinc containing fluoridated hydroxyapatite (ZnFHA) films on Ti6Al4V substrates was prepared using sol-gel dip-coating method. The release of zinc ions from ZnFHA film was controlled mainly by the zinc content in the film. The release behavior showed an initial rapid increase release followed by a tapering-off and directed to a constant value at longer time. After soaking in SBF for 8 days, a layer was deposited and completely covered the original surface of the ZnFHA film, indicating good in vitro "bioactivity." The osteoblast-like MG63 cells were seeded on the ZnFHA films; FHA film and Ti6Al4V substrate were used as control. The cell culture result showed that cell adhesion and proliferation on ZnFHA films were significantly increased compared with the controls. The results in this work suggest that ZnFHA films on Ti6Al4V substrates can function as an implant with good bioactivity and cytocompatibility.

Composites of gellan gum hydrogel enzymatically mineralized with calcium-zinc phosphate for bone regeneration with antibacterial activity

“…This suggests that Zn may have aided cell adhesion and early proliferation. There have been several reports describing superior adhesion and early proliferation of a range of cells, including rat calvarial osteoblasts, fibroblast‐like cells, osteoblast‐like cells and mesenchymal stem cells, on Zn‐substituted HA materials with Zn contents ranging up to 2 mol% (Webster et al ., ; Chung et al ., ; Miao et al ., ; Thian et al ., ). The molar and mass percentages of Zn in the mineral formed in sample groups B and C were approximately an order of magnitude higher than those in the aforementioned studies (Tables and 3), in the dry state.…”

“…Zinc is gaining in interest as a component of biomaterials for bone regeneration, for several reasons (Habibovic et al ., ; Mourino et al ., ). First, as a component of apatite‐based materials, zinc has supported the proliferation of mesenchymal stem cells (MSCs) (Thian et al ., ) and the adhesion, proliferation and osteogenic differentiation of osteoblast‐like cells (Webster et al ., ; Yang et al ., ; Miao et al ., ; Wang et al ., ) and pre‐osteoblasts (Storrie and Stupp, ). Second, as a component of tricalcium phosphate (TCP), biphasic TCP–hydroxyapatite (HA) and brushite ceramics, zinc has increased new bone formation and bone–implant contact in vivo (Kawamura et al ., , ; Li et al ., ; Pina et al ., ); Zn‐enriched TCP has also stimulated ectopic bone formation (Luo et al ., ).…”

Reinforced Portland cement porous scaffolds for load‐bearing bone tissue engineering applications

“…SEM imaging [Figure 3(a–f)] revealed that the cells were able to adhere, spread, and propagate on the scaffold external and internal surfaces (i.e., trabecules and pore walls). The cells generally exhibited a cuboidal‐like morphology typical of mature osteoblasts,34–37 and numerous filopodia interacting with other cells and the micro/nanostructured surface of the cement [Figure 3(c,d)]. Multilayered lamellar‐like structures formed by cells and presumably other extracellular matrix components were also observed in some instances [Figure 3(f)].…”

Polyurethane/fluor-hydroxyapatite nanocomposite scaffolds for bone tissue engineering. Part I: morphological, physical, and mechanical characterization

“…In addition, FHA keeps comparable biocompatibility and bioactivity with pure HA in terms of the tissue and cell response. 17,18 The HA powder has been synthesized over the past years using different techniques, such as precipitation, hydrothermal, and sol-gel methods. 19 Among these, the sol-gel technique offers many advantages over other techniques, such as high chemical homogeneity, fine grain structure, and low crystallization temperature, and by being both an economical and technically simple procedure to perform.…”