In this study, new nano-fluor-hydroxyapatite (nFHA)/polyurethane composite scaffolds were fabricated for potential use in bone tissue engineering. Polyester urethane samples were synthesized from polycaprolactone, hexamethylene diisocyanate, and 1,4-butanediol as chain extender. Nano fluor-hydroxyapatite (nFHA) was successfully synthesized by sol-gel method. The solid–liquid phase separation and solvent sublimation methods were used for preparation of the porous composites. Mechanical properties, chemical structure, and morphological characteristics of the samples were investigated by compressive test, Fourier transform infrared, and scanning electron microscopy (SEM) techniques, respectively. The effect of nFHA powder content on porosity and pore morphology was investigated. SEM images demonstrated that the scaffolds were constituted of interconnected and homogeneously distributed pores. The pore size of the scaffolds was in the range 50–250 μm. The result obtained in this research revealed that the porosity and pore average size decreased and compressive modulus increased with nFHA percentage. Considering morphological, physical, and mechanical properties, the scaffold with a higher ratio of nFHA has suitable potential use in tissue regeneration.
Bioactive materials can attract calcium and phosphate ions in simulated body fluid (SBF) solution to mimic the composition of extracellular matrix (ECM). Rapid biodegradation rate of natural polymers in contact with water-based solutions and time-consuming process of mineralization in SBF led to using concentrated simulated media. Herein, gelatin-siloxane microspheres were fabricated via single emulsion method. Then hybrid spheres were immersed in the modified 10X-SBF solution, and microwave energy (600 W) was expanded for the rapid formation of hydroxyapatite (HA) on the spheres. Results indicated homogeneous coating of microspheres and high similarity of synthesized HA to the bone composition. Increasing intensity of HA-related peaks in Fourier transform infrared spectrum, X-ray diffraction and surface roughness after utilizing microwave-assisted method confirmed high efficiency of this technique in biomimetic mineralization of structures. Cell culture studies with human osteosarcoma cell lines (MG-63) demonstrated that mineralized HA in 10X-SBF solution under microwave treatment could be able to mimic bone ECM for tissue regeneration applications in the shortest time and highest similarity to the natural tissue.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.