Immunogenicity and protection induced by recombinant Toxocara canis proteins in a murine model of toxocariasis

Salazar-Garcés, Luis F.; Santiago, Leonardo Freire; Santos, Sara Patrícia de Oliveira; Hernández, Dumar Alexander Jaramillo; Silva, Márcia Barbosa da; Alves, Vitor dos Santos; Silveira, Elisânia Fontes; Barrouin‐Melo, Stella Maria; Cooper, Philip J.; Pacheco, Luis Gustavo Carvalho; Pinheiro, Carina S.; Alcantara-Neves, Neuza Maria

doi:10.1016/j.vaccine.2020.04.072

Cited by 10 publications

(9 citation statements)

References 57 publications

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“…Aspects of the immune response of nonhuman paratenic hosts, with possible benefits for surveys aimed at determining the frequency of infections by Toxocara larvae, have been studied in vivo, in mouse or rabbit models. [80][81][82][83][84][85][86][87][88][89][90] Serological surveys carried out on synanthropic animals that are paratenic hosts of T. canis revealed significant natural frequencies of infection, indicating that there is a risk involved in the human consumption of raw or undercooked meat from these animals. [91][92][93][94] Recombinant proteins used in ELISAs and Western blot assays for the diagnosis of Toxocara infections of cattle, horses, and sheep have obtained good results.…”

mentioning

confidence: 99%

Human Toxocariasis: 2010 to 2020 Contributions from Brazilian Researchers

Chieffi¹,

Lescano²,

Fonseca³

et al. 2021

RRTM

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mentioning

confidence: 99%

Human Toxocariasis: 2010 to 2020 Contributions from Brazilian Researchers

Chieffi¹,

Lescano²,

Fonseca³

et al. 2021

RRTM

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“…However, our predictions and the protection achieved with the As37 recombinant protein support the idea that only targeting secreted proteins can be detrimental to the selection of good vaccination targets in Ascaris. Recent work in Toxocara canis, a parasite of the same family as Ascaris, showed that membrane proteins are capable to induce protection in a mouse model, reinforcing the idea that it is an error to disregard these proteins when looking for vaccination targets in nematodes (Salazar Garcés et al, 2020).…”

Section: Discussionmentioning

confidence: 94%

“…This methodology allows researchers to focus down vaccination assays to a smaller set of proteins, effectively reducing costs and time. This reverse vaccinology approach has been successfully applied to identify vaccination targets for other nematodes, for example T. canis (Salazar Garcés et al, 2020) and T. muris (Zawawi et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

“…Several annotated genomes are now available for Ascaris lumbricoides and Ascaris suum , which enable the search for putative vaccine candidates using a reverse vaccinology analysis. A reverse vaccinology approach combines genome information and bioinformatic tools for identification of vaccine candidates, and has been successfully applied to other nematodes before, such as Toxocara canis and Trichuris muris (Salazar Garcés et al, 2020; Zawawi et al, 2020). Such methodology allows researchers to uncover vaccine targets from predicted proteomes by assessing if proteins have useful characteristics.…”

Section: Introductionmentioning

confidence: 99%

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A reverse vaccinology approach identifies putative vaccination targets in the zoonotic nematode Ascaris

Evangelista

Vliet

Lawton

et al. 2022

Preprint

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Ascariasis is the most prevalent helminthic disease affecting both humans and pigs and is caused by the roundworms Ascaris lumbricoides and Ascaris suum. While preventive chemotherapy continues to be the most common control method, recent reports of anthelminthic resistance highlight the need for development of a vaccine against ascariasis. The aim of this study was to use a reverse vaccinology approach to identify potential vaccine candidates for Ascaris. Three Ascaris proteomes predicted from whole-genome sequences were analysed. Candidate proteins were identified using open-access bioinformatic tools (e.g. Vacceed, VaxiJen, Bepipred 2.0) which test for different characteristics such as sub-cellular location, T-cell and B-cell molecular binding, antigenicity, allergenicity and phylogenetic relationship with other nematode proteins. From over 100,000 protein sequences analysed, four transmembrane proteins were predicted to be non-allergen antigens and potential vaccine candidates. The four proteins are a Piezo protein, two voltage-dependent calcium channels and a protocadherin-like protein, are all expressed in either the muscle or ovaries of both Ascaris species, and all contained high affinity epitopes for T-cells and B-cells. The use of a reverse vaccinology approach allowed the prediction of four new potential vaccination targets against ascariasis in humans and pigs. These targets can now be further tested in in vitro and in vivo assays to prove efficacy in both pigs and humans.

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Section: Figurementioning

confidence: 94%

A reverse vaccinology approach identifies putative vaccination targets in the zoonotic nematode Ascaris

Evangelista

Vliet²,

Lawton³

et al. 2022

Front. Vet. Sci.

View full text Add to dashboard Cite

Ascariasis is the most prevalent helminthic disease affecting both humans and pigs and is caused by the roundworms Ascaris lumbricoides and Ascaris suum. While preventive chemotherapy continues to be the most common control method, recent reports of anthelminthic resistance highlight the need for development of a vaccine against ascariasis. The aim of this study was to use a reverse vaccinology approach to identify potential vaccine candidates for Ascaris. Three Ascaris proteomes predicted from whole-genome sequences were analyzed. Candidate proteins were identified using open-access bioinformatic tools (e.g., Vacceed, VaxiJen, Bepipred 2.0) which test for different characteristics such as sub-cellular location, T-cell and B-cell molecular binding, antigenicity, allergenicity and phylogenetic relationship with other nematode proteins. From over 100,000 protein sequences analyzed, four transmembrane proteins were predicted to be non-allergen antigens and potential vaccine candidates. The four proteins are a Piezo protein, two voltage-dependent calcium channels and a protocadherin-like protein, are all expressed in either the muscle or ovaries of both Ascaris species, and all contained high affinity epitopes for T-cells and B-cells. The use of a reverse vaccinology approach allowed the prediction of four new potential vaccination targets against ascariasis in humans and pigs. These targets can now be further tested in in vitro and in vivo assays to prove efficacy in both pigs and humans.

show abstract

Immunogenicity and protection induced by recombinant Toxocara canis proteins in a murine model of toxocariasis

Cited by 10 publications

References 57 publications

Human Toxocariasis: 2010 to 2020 Contributions from Brazilian Researchers

Human Toxocariasis: 2010 to 2020 Contributions from Brazilian Researchers

A reverse vaccinology approach identifies putative vaccination targets in the zoonotic nematode Ascaris

A reverse vaccinology approach identifies putative vaccination targets in the zoonotic nematode Ascaris

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