“…Although most plants can adapt to these environmental conditions, some of them become abnormal with a translucent aspect due to chlorophyll deficiency and high water content (Debergh, 1983;Gaspar, 1991). The phenomenon has been considered as a morpho-physiology disorder and many physiological and biochemical changes have been observed (Ziv, 1991;Franck et al, 2004). Some of these factors include the presence in the medium of growth regulators, of large quantities of NH4 + and Cl -ions, the type and concentration of the gelling agent, high relative humidity in the culture vessels (Debergh et al, 1983;Ziv, 1991;Kevers et al, 2004;Hazarika, 2006) and the successive subcultures of the explants in a culture medium with cytokinin (Vieitez et al, 1985).…”