Oxidative Stress Studies in Plant Tissue Culture

Şen, Ayşe

doi:10.5772/48292

Cited by 33 publications

(30 citation statements)

References 75 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Different ROS produced in plant cells [35] are controlled by different antioxidants and enzymatic and non-enzymatic antioxidant defense pathways. The enzymatic antioxidants pathway include CAT, SOD, APX, POD, GR, and DHAR [36]. In the current study, CAT activity in milk thistle grew under drought stress was significantly higher at 18 WAP.…”

Section: Discussionsupporting

confidence: 47%

The Impact of Drought Stress on Antioxidant Responses and Accumulation of Flavonolignans in Milk Thistle (Silybum marianum (L.) Gaertn)

ElSayed

El-hamahmy

Rafudeen

et al. 2019

Plants

View full text Add to dashboard Cite

Biosynthesis and accumulation of flavonolignans in plants are influenced by different environmental conditions. Biosynthesis and accumulation of silymarin in milk thistle (Silybum marianum L.) were studied under drought stress with respect to the antioxidant defense system at the physiological and gene expression level. The results revealed a reduction in leaf chlorophyll, ascorbic acid, and glutathione contents. In contrast, H2O2, proline, and antioxidative enzyme activities, such as superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), ascorbate peroxidase (APX), and glutathione reductase (GR), were increased. These results confirmed that milk thistle undergoes oxidative stress under drought stress. Furthermore, transcription levels of APX, SOD, CAT, 1-Cys-Prx, and PrxQ were significantly increased in milk thistle under drought stress. Overall this suggests that protection against reactive oxygen species and peroxidation reactions in milk thistle are provided by enzymatic and non-enzymatic antioxidants. Flavonolignans from milk thistle seeds after different drought treatments were quantified by high-performance liquid chromatography (HPLC) and showed that severe drought stress enhanced the accumulation of silymarin and its components compared with seeds from the control (100% water capacity). Silybin is the major silymarin component and the most bioactive ingredient of the milk thistle extract. Silybin accumulation was the highest among all silymarin components in seeds obtained from drought-stressed plants. The expression of the chalcone synthase (CHS) genes (CHS1, CHS2, and CHS3), which are associated with the silybin biosynthetic pathway, was also increased during drought stress. These results indicated that milk thistle exhibits tolerance to drought stress and that seed derived from severe drought-stressed plants had higher levels of silymarin.

show abstract

Section: Discussionsupporting

confidence: 47%

The Impact of Drought Stress on Antioxidant Responses and Accumulation of Flavonolignans in Milk Thistle (Silybum marianum (L.) Gaertn)

ElSayed

El-hamahmy

Rafudeen

et al. 2019

Plants

View full text Add to dashboard Cite

show abstract

“…Their productions are controlled by various enzymatic and non-enzymatic antioxidant defense systems. Enzymatic antioxidant defense systems, including CAT, APX, POX, SOD, MDHAR, DHAR and GR (Sen, 2012).…”

Section: Figmentioning

confidence: 99%

Effects of drought stress on physiological and biochemicalchanges in Phaseolus vulgaris L.

Kuşvuran

Daşgan

2017

View full text Add to dashboard Cite

The present study investigated different levels (S1: 100% of field capacity-Control S2: 50% of field capacity-moderate stress; S3: 0% of field capacity-severe stress) of drought stress on oxidative damages and variations in antioxidants in the two bean varieties Bn-16 (drought-sensitive), to elucidate the antioxidative protective mechanism governing differential drought tolerance. The shoot fresh weight, shoot height, leaf number and area, RWC were reduced with different level of drought stress. However this reduction clearly occurred in Bn-16 (sensitive). Antioxidative enzyme activities, such as superoxide dismutase, catalase, ascorbate peroxidase and glutation reductase, had a greater increase in tolerant genotypes (Bn-150) than in sensitive ones (Bn-16). The level of lipid peroxidation was measured by estimating malondialdehyde content. Lipid peroxidation increased with increasing drought conditions in all genotypes, although Bn-150 was the least affected when compared with the other genotype. Total phenolic and flavonoid content increased in bean genotypes under S2 and S3 conditions. The highest total phenolic and flavonoid contents were attained in Bn-150 subjected to S3 treatment. These results indicated that an antioxidant defence system, osmolytes (such as proline), and secondary metabolites play important roles in common bean (Phaseolus vulgaris L) during drought stress and recovery.

show abstract

“…Straight a consequence of stress is the induction of stress antioxidant enzymes by exposed plants to minimize damage caused by reactive oxygen species [44]. Enzymatic antioxidant defense systems, such as APX, CAT, DHAR, GR, MDHAR, POX, SOD and non-enzymatic antioxidant defense systems, like ascorbate, carotenoids, glutathione, glycine betain, phenolic compounds, polyamines, proline, and sugar [40,45]. The most of results indicated that due to drought stress, APX, CAT, and SOD activities are positively related to drought tolerance [37,40,44].…”

Section: Ilns Biostimulation and Overcoming The Abiotic Stresses In Pmentioning

confidence: 99%

Using of Microbial Fertilizer as Biostimulant Alleviates Damage from Drought Stress in Guar (<i>Cyamopsis Tetragonoloba</i> (L.) Taub.) Seedlings

Kuşvuran

2019

ILNS

View full text Add to dashboard Cite

Drought is a significant environmental stress that limits plant growth and yield. In this study, an investigation of guar, grown under different drought level conditions [(S0: 100% of field capacity), S1 (depletion of 75% the available water holding capacity), S2 (depletion of 50% the available water holding capacity), S3 (depletion of 25% the available water holding capacity), S4 (no applied irrigation water)] with regards to the impact of Chlorella vulgaris based microbial fertilizer on physiological, morphological, and enzymatic activity was performed. Microbial fertilizer applications significantly increased shoot length, fresh and dry weight of the shoot and root, and leaf number and area of guar plants compared to the only drought stress treatments. In addition, following the above-mentioned procedures, there were significant increases in the relative water content, total phenolic and flavonoid contents, superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), and glutation reductase (GR) activity. However, the malondialdehyde (MDA) content were significantly decreased. Hence, the results support the administration of a foliar application to the microbial fertilizer containing microalgae in order to increase the guar plant’s defense system, enabling it to tolerate the negative effects resulting from drought stress.

show abstract

Oxidative Stress Studies in Plant Tissue Culture

Cited by 33 publications

References 75 publications

The Impact of Drought Stress on Antioxidant Responses and Accumulation of Flavonolignans in Milk Thistle (Silybum marianum (L.) Gaertn)

The Impact of Drought Stress on Antioxidant Responses and Accumulation of Flavonolignans in Milk Thistle (Silybum marianum (L.) Gaertn)

Effects of drought stress on physiological and biochemicalchanges in Phaseolus vulgaris L.

Using of Microbial Fertilizer as Biostimulant Alleviates Damage from Drought Stress in Guar (<i>Cyamopsis Tetragonoloba</i> (L.) Taub.) Seedlings

Contact Info

Product

Resources

About