HPLC methods for quality control of Moringa oleifera extract using isothiocyanates and astragalin as bioactive markers

Abstract

Herbal extracts are gaining widespread acceptance as ingredients in cosmetic and health products. Moringa oleifera Lam. is an interesting candidate as it has multiple biological properties. However, methods for quality control of the use of M. oleifera are still required. This study proposes the use of isothiocyanate compounds and astragalin as chemical markers to standardize M. oleifera extracts. Both of these compounds are known to have anti-inflammation properties, and astragalin is also reported to have antioxidant properties. The HPLC-based cyclocondensation method was further developed and validated specifically for quantitative analysis of the isothiocyanate compounds in the M. oleifera extracts, and the concentration of astragalin in the M. oleifera extracts was determined by the HPLC method. Both methods gave acceptable linearity, accuracy, and precision with the limit of detection of 0.13 µg/ml for total isothiocyanate and 0.10 µg/ml for astragalin. The uses of the methods were demonstrated in the analyses of the components in the extracts taken variously from the leaves, immature seeds, mature seeds, petioles, and pods of the M. oleifera tree. Our results suggest that the methods developed could be used in the quality control of M. oleifera extracts to develop cosmetic and natural health products.

Evaluation of Anti-Inflammatory Effect of Moringa oleifera Lam. and Cyanthillium cinereum (Less) H. Rob. Lozenges in Volunteer Smokers

“…Astragalin, a bioactive natural flavonoid has been well known for multiple pharmacological properties including antioxidant, anti-inflammation, anti-cancer, and cardioprotective property [ 37 ]. Furthermore, astragalin has been used as a chemical marker for quality control and standardization of MO extract using the high performance liquid chromatography (HPLC) method [ 38 ]. The quality control of plant extraction and its products can be done by checked the present of an active ingredient.…”

“…Residual solvent of ethanol in extract was measured using GC/MS with headspace technique by Central Laboratory (Thailand) Co., Ltd. Astragalin was used as the chemical marker of MO and CC extracts. Astragalin is also known to have antioxidant properties [ 38 ]. Hence, astragalin was used as a bioactive marker for quality control and standardization of extract and lozenge products.…”

“…Astragalin also has antioxidant property [ 37 ]. This compound has been used as a chemical marker for quality control and standardization of MO extract using the high performance liquid chromatography (HPLC) method [ 38 ].…”

Improvements of alveolar bone healing using Moringa oleifera leaf powder and extract biomimetic composite: an experimental study in dogs

“…Going into some details, the MO leaf extract increased alkaline phosphatase activity, collagen type I expression, osteocalcin and transforming growth factor beta (TGF B ) expressions (responsible for deposition of bone matrix ), besides it decreased number of Rankle expression . 1,24,29,31,32 Moreover, a study conducted on upper central extraction socket in a rat model 2018 using mate tea intra-gastric lavage concluded positive effects on alveolar healing at 28 days postoperatively. 4 Our study uniquely used high concentration of water extract (11.7%) suggested to be comparable to ethanol osteoprotective property.…”

Immunomodulatory Effects of Formulation of Channa micropeltes and Moringa oleifera through Anti-Inflammatory Cytokines Regulation in Type 1 Diabetic Mice

“…MO leaves were extracted using water according to Engsuwan 20 and El-Gammal 21 with modification. The extraction of MO leaves was performed by dissolving 5 grams leaves powder of MO (Materia Medica Batu, Malang, Indonesia) in 50 mL of boiled water.…”