This study aimed to evaluate the effect of Moringa-Albumin (MA) combination on pro-inflammatory cytokine expressions, especially IFN-γ and TNF-α, in a diabetic mouse model. Streptozotocin with a 145 mg/kg BW dose was used to induce diabetes condition in BALB/c mice. Mice with positive DM (blood glucose levels ≥ 200 mg/dL) were orally administered with MA for 14 days at dose 1, dose 2, and dose 3. On day 15th, spleen cells were isolated to analyze IFN-γ and TNF-α expressions by flow cytometry. The data were statistically analyzed with one-way ANOVA (ρ≤ 0.05) and Tukey test using SPSS version 16 for Windows. The results showed that the MA combination had anti-inflammatory activity in inhibiting IFN-γ and TNF-α. Furthermore, dose 1 affected to decrease in the IFN-γ expression while dose 3 decreased the expression of TNF-α. Thus, it can be concluded that the MA combination has a role in inhibiting IFN-γ and TNF-α in a dosage-dependent manner. Based on the results, we assumed that MA might be one of the biological materials with efficacy to treat DM patients.
CLE can suppress pro-inflammatory cytokines in hyperglycemic mouse model, suggesting this medicinal herb may be beneficial as a potential strategy for future therapeutic interventions in degenerative diseases or a disease involving cell activation.
Context: Acute myeloid leukemia (AML) is the most common form of acute leukemia. Currently, many people use medicinal herbs for the treatment of cancers. Nut grass (Cyperus rotundus L.) is a medicinal plant widely used in conventional medicine due to its role as an anti-cancer. Aims: To evaluate the effect of Cyperus rotundus tuber (CRT) ethanolic extract on cell proliferation, differentiation, cell cycle, and apoptotic of HL-60 cells. Methods: HL-60 cells line as a model for AML was cultured under the influence of CRT extract concentrations (35.4, 354, and 3540 µg/mL) for 48 h. Furthermore, the cell was subjected to carboxyfluorescein succinimidyl ester staining for proliferation, using a CD117 marker for differentiation. Annexin V-FITC and PI for cell cycle and apoptosis. The effect of this herb was studied by flow cytometry. The data were statistically analyzed with one-way ANOVA (p≤0.05) and the Tukey test using SPSS version 16 for Windows. Results: The results showed that the CRT inhibited proliferation activity, differentiation, cell cycle arrest, and apoptosis. Conclusions: The data clearly showed the potential anti-cancer activity of CRT on HL-60 cells and suggested that it could help develop promising therapeutic agents for AML treatments.
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