HPLC-based metabolomics to identify cytotoxic compounds from Plectranthus amboinicus (Lour.) Spreng against human breast cancer MCF-7Cells

Yulianto, Wahid; Andarwulan, Nuri; Giriwono, Puspo Edi; Pamungkas, Joko

doi:10.1016/j.jchromb.2016.10.024

Cited by 25 publications

(21 citation statements)

References 22 publications

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“…anti-proliferative activity in WiDr cancer cells and has a potential as chemotherapeutic agents. A previous study has demonstrated anti-proliferative effect of P. amboinicus leaves using MTT assay on MCF-7 human mammary cancer cells with the most active fraction was from chloroform fraction (IC 50 value of 2.46 µg/ml) and the hexane fraction was 8.85 µg/ ml) [10].…”

Section: Gene Expressionmentioning

confidence: 93%

“…Significant anticonvulsant activity of C. amboinicus in Swiss Albino Mice was also reported and the presence of flavonoids, alkaloids, and saponin in the extract may be responsible for this activity [9]. Plectranthus amboinicus Lour also had a cytotoxic effect on HeLa cells [10]. However, the activity and the mechanism of action against colon cancer cell in different concentrations of extract have not been performed.…”

Section: Introductionmentioning

confidence: 99%

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Methanol Extract of Coleus amboinicus (Lour) Exhibited Antiproliferative Activity and Induced Programmed Cell Death in Colon Cancer Cell WiDr

Laila

Fardiaz

Yuliana

et al. 2020

International Journal of Food Science

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Coleus amboinicus(Lour) (CA) has been reported to possess many pharmacological activities. In this study, evaluation of cytotoxicity using brine shrimp lethality bioassay and MTT assay using WiDr cell lines was carried out. The expression of several genes responsible for programmed cell death of the methanol extract of CA was also investigated. The morphology of the cells undergoing apoptosis was detected using Hoechst staining assay. The gene expression of BAX, BCL2, P53, Caspase 1, 7, 8, and 9 of treated samples with different concentrations (10, 15, 25 & 50 µg/ml) were measured with RT PCR. The phytochemical profiles were investigated using LC MS. The results showed that the lethality concentration (LC50) of methanol extract using brine shrimp was 34.545 µg/ml and the extract exhibited good antiproliferative activity against cancer cells WiDr with IC50 value (8.598 ± 2.68 µg/ml) as compared to standard drug 5-fluorouracil (IC50 value 1.839 ± 0.03 µg/ml). There was apoptotic evidences from the morphology of treated cells. The expressions of BAX,P53, and Caspase 9 were upregulated in lower concentration of the extract (10 and 15 µg/ml) but downregulated in higher concentration (25 and 50 µg/ml). BCL2 as anti-apoptotic gene was downregulated in all concentrations. Caspase 1 and Caspase 7 were upregulated in high concentration (25 and 50 µg/ml), but downregulated in lower concentrations. These data provide a mode of cell death for the methanol extract of CA in low concentrations corresponding to apoptosis with intrinsic pathway. Many valuable compounds identified including caffeic acid, rosmarinic acid, malic acid, eicosapentanoic acid, benserazide, alpha-linolenic acid, betaine, Salvanolic B, 4-hydroxibenzoic acid and firulic acid have been previously reported as being active agents against many cancer cells. This study suggested that CA might become an effective ingredient for health-beneficial foods to prevent colon cancer.

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Section: Gene Expressionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Methanol Extract of Coleus amboinicus (Lour) Exhibited Antiproliferative Activity and Induced Programmed Cell Death in Colon Cancer Cell WiDr

Laila

Fardiaz

Yuliana

et al. 2020

International Journal of Food Science

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“…Daun tanaman torbangun biasanya dikonsumsi dalam bentuk sup segar (Damanik et al, 2006;Damanik 2009). Beberapa penelitian lain menunjukkan adanya potensi daun torbangun sebagai obat herbal, diantaranya sebagai antikanker (Yulianto et al 2016), antibakteri dan antibiofilm (Vijayakumar et al 2015), diuretik, analgesik, sitotoksisitas, anti-inflamasi, dan antioksidan (El-hawary et al 2012). Khasiat farmakologi suatu tanaman obat ditentukan oleh fitokimia yang terkandungnya.…”

Section: Pendahuluanunclassified

Analisis komparatif kandungan metabolit pada daun mutan tanaman Torbangun (Plectranthus amboinicus (Lour.) Spreng.)

et al. 2020

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Torbangun (Plectranthus amboinicus) merupakan tanaman obat dari keluarga Lamiaceae. Pada masyarakat Batak, daun torbangun digunakan untuk meningkatkan produksi air susu. Keragaman kandungan fitokimia dapat ditingkatkan salah satunya dengan teknik iradiasi sinar gamma untuk program pemuliaan tanaman. Pada penelitian ini, metode metabolomik yang tidak tertarget digunakan untuk mengevaluasi profil senyawa pada bagian daun tanaman mutan dari iradiasi sinar gamma dan kontrol. Kandungan senyawa planlet mutan dan kontrol dianalisis dengan GC-MS. Data GC-MS dianalisis dengan kemometrik dengan menggunakan hierarchical cluster analysis (HCA). Keragaman kandungan kimia ditunjukkan oleh planlet mutan dan kontrol daun torbangun. Hasil analisa planlet kontrol torbangun menunjukkan terdapat lima senyawa utama diantaranya hydroxymethylfurfurole (35,15%), brevifolin (7,69%), 3-Dihydro-3,5-Dihydroxy-6-Methyl-4H-Pyran-4-one (DDMP) (13,13%), stigmasterol (6,51%) dan ferruginol (8.63%). Hasil analisa mutan torbangun memiliki kandungan senyawa DDMP (19,57%), neophytadiene (8,47%), linolenic acid (9,10%), ferruginol (7,61%), stigmasterol (14,14%) dan gamma.-sitosterol (7,08%). HCA menunjukkan tiga komponen senyawa yang berbeda antara planlet kontrol dan mutan yaitu kenaikan kandungan senyawa DDMP dan stigmasterol, serta penurunan kandungan hydroxymethylfurfurole pada mutan torbangun.

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“…The total extract showed cytotoxic activity against MCF-7, using HPLC-based metabolomics approach, and 7α-acetoxy-6β-hydroxyroyleanone (102) was identified as the main active constituent. Other minor compounds like coleon E (103) and royleanone (104) were also identified [51]. Fatty acid esters of 7α-acyloxy-6β-hydroxyroyleanone (152) showed moderate antibacterial activity [62]; coleon U exhibited potent cytotoxicity against a panel of human cancer cell lines [63,65] also showed potent inhibition of mouse splenocyte proliferation induced by ConA or LPS mitogens [64].…”

Section: Plectranthus Amboinicusmentioning

confidence: 99%

Chemistry of South African Lamiaceae: Structures and Biological Activity of Terpenoids

Hussein¹

2018

Terpenes and Terpenoids

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South Africa flora is one of the most important mega floras with high endemic species percentage. Lamiaceae is an important family in South Africa with ±308 species in 41 genera and contains many important plants (~23%) traditionally used for treatment of different human diseases. The chemical profile of Lamiaceae is very rich in terpenoids in general and more specifically diterpenes. Genera like Leonotis and Plectranthus are well studied, while on the other hand, genus like Stachys (~41 species, ~50% endemic) didn't receive any attention. Different classes of diterpenes were identified and some of them demonstrating important biological activities.

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HPLC-based metabolomics to identify cytotoxic compounds from Plectranthus amboinicus (Lour.) Spreng against human breast cancer MCF-7Cells

Cited by 25 publications

References 22 publications

Methanol Extract of Coleus amboinicus (Lour) Exhibited Antiproliferative Activity and Induced Programmed Cell Death in Colon Cancer Cell WiDr

Methanol Extract of Coleus amboinicus (Lour) Exhibited Antiproliferative Activity and Induced Programmed Cell Death in Colon Cancer Cell WiDr

Analisis komparatif kandungan metabolit pada daun mutan tanaman Torbangun (Plectranthus amboinicus (Lour.) Spreng.)

Chemistry of South African Lamiaceae: Structures and Biological Activity of Terpenoids

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