Analisis komparatif kandungan metabolit pada daun mutan tanaman Torbangun (Plectranthus amboinicus (Lour.) Spreng.)

Aisyah, Syarifah Iis; Rusmiyati, Henny; Sukma, Dewi; Damanik, Rizal; Nurcholis, Waras

doi:10.33019/agrosainstek.v4i1.109

Cited by 2 publications

(2 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Based on morphological changes, one putative mutant for P. amboinicus was produced from the study. Another study reported using gamma-ray irradiation to evaluate the compounds profile from the leaves of P. amboinicus mutant compared with its wild-type (Aisyah et al 2020). From the hierarchical cluster analysis (HCA), they found that wild-type and mutant plantlets differed in their 2,3dihydro-3,5-dihydroxy-6-methyl-4H-pyran-4-one (DDMP), stigmasterol, and hydroxymethylfurfural contents.…”

Section: Introductionmentioning

confidence: 99%

Performance of Putative Mutants and Genetic Parameters of Plectranthus amboinicus (L.) through Mutation Induction With Colchicine

Wibisono

Aisyah²,

Nurcholis³

et al. 2021

agrosainstek

Self Cite

View full text Add to dashboard Cite

Genetic enhancement in vegetatively propagated crops can be done through mutation induction. Colchicine-induced mutation is one of the methods that can be employed to increase plant genetic diversity. This study aimed to determine the effect of colchicine on the performance and genetic parameters of MV3 generation of Plectranthus amboinicus (L.). This study was conducted at the Laboratory of Agricultural Seed Management, Plantation Research and Development Center, from June 2018 to June 2019. Nodes and shoots were used as explants. Mutation induction was performed using colchicine at concentrations of 0%, 0.02%, 0.04%, and 0.06%. Explant regeneration and subculture were done on MS0 medium. The number of plantlets yielded were 59 (0% concentration of colchicine), 60 (0.02%), 81 (0.04%), and 80 plantlets (0.06%), respectively. Results indicated that colchicine-induced mutation in an in vitro culture was able to generate high genetic diversity in both quantitative and qualitative characters of the plantlets. At the concentration of 0.04%, colchicine produced the highest frequency of putative mutants (28.4%). Genetic parameters in MV3 generation of P. amboinicus plantlets showed that five quantitative characters, i.e. plantlet height, number of leaves, number of shoots, leaf length, and number of roots had high heritability values at a concentration around the LC50 value (0.0275%).

show abstract

Section: Introductionmentioning

confidence: 99%

Performance of Putative Mutants and Genetic Parameters of Plectranthus amboinicus (L.) through Mutation Induction With Colchicine

Wibisono

Aisyah²,

Nurcholis³

et al. 2021

agrosainstek

Self Cite

View full text Add to dashboard Cite

show abstract

“…Coleus amboinicus is a member of the family of Lamiaceae, known to contain many clinically useful bioactive compounds such as flavonoids, alkaloids, tannins, triterpenoids and saponins (Damanik et al 2017;Aisyah et al 2020). This medicinal plant in Indonesia is called torbangun and has been traditionally used by Bataknese as a stimulant for breast milk (a lactagogue) (Damanik 2009).…”

Section: Introductionmentioning

confidence: 99%

Effect of light exposure on secondary metabolites production of an endophytic fungus Arthrinium rasikravindrae and its antioxidant and anticancer activities

et al. 2021

View full text Add to dashboard Cite

Abstract. Eltivitasari A, Rahmawati, Gemantari BM, Romadhonsyah F, Nurrochmad A, Wahyuono S, Astuti P. 2021. Effect of light exposure on secondary metabolites production of an endophytic fungus Arthrinium rasikravindrae and its antioxidant and anticancer activities. Biodiversitas 22: 3156-3163. Endophytic microorganisms are one of the promising sources in producing bioactive compounds, to be developed for new drug candidates. They are found to have the ability to generate the same compounds as their host plant. Metabolite producing capacity of the endophytes is known to be affected by light exposure during fermentation process. This study focused on an endophytic fungus Arthrinium rasikravindrae isolated from Coleus amboinicus stem to reveal out its metabolite profiles due to light exposure as well as its bioactivity consequences. A. rasikravindrae was cultured on potato dextrose broth medium for 14 days and fermented in dark and exposed to natural light. Metabolite profiling was performed using TLC and GC-MS analysis. The activities were observed using DPPH assay for antioxidant and MTT assay for cytotoxicity potential. The results showed that A. rasikravindrae ethyl acetate extract produced during dark and exposed to light fermentation conditions contained different compounds but there was some which showed similarity with their host plant. Methyl octadec-9-enoate was found in all fermentation conditions as well as in C. amboinicus stem extract. Besides methyl octadec-9-enoate, methyl palmitate was also found present in both A. rasikravindrae extract fermented exposed to light and its host plant. The antioxidant activity of extract generated from dark fermentation condition was better as compared to that exposed to light with IC50 value of 66.36±0.53 vs 556.92±34.37 µg/mL. However, cytotoxic activity screening against several cancer cell lines exhibited opposing results in which extract from light-exposed fermentation resulted in better cytotoxic activity (IC50 value of 291.40 ± 2.34 µg/mL on WiDr, 336.80 ± 5.05 µg/mL on T47D, and 404.73 ± 3.46 µg/mL on Hela cell lines). Extract obtained from dark fermentation condition showed IC50 value of more than 500 µg/mL in all tested cancer cell lines. Preliminary examination on cytotoxic activity against WiDR cells suggested that the extract from light-exposed fermentation might induce cell death through mechanisms involving cell cycle arrest.

show abstract

Analisis komparatif kandungan metabolit pada daun mutan tanaman Torbangun (Plectranthus amboinicus (Lour.) Spreng.)

Cited by 2 publications

References 25 publications

Performance of Putative Mutants and Genetic Parameters of Plectranthus amboinicus (L.) through Mutation Induction With Colchicine

Performance of Putative Mutants and Genetic Parameters of Plectranthus amboinicus (L.) through Mutation Induction With Colchicine

Effect of light exposure on secondary metabolites production of an endophytic fungus Arthrinium rasikravindrae and its antioxidant and anticancer activities

Contact Info

Product

Resources

About