Histopathological findings and detection of parasites in the eyes of dogs infected naturally with Leishmania chagasi

Brito, Fábio Luiz da Cunha; Laus, José Luiz; Tafuri, Wagner Luiz; Figueiredo, María Marta; Júnior, Valdemiro Amaro Silva; Maia, Frederico Celso Lyra; Alves, Lêucio Câmara

doi:10.1590/s0103-84782010005000079

Cited by 11 publications

(32 citation statements)

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“…Anterior uveitis, as other ocular changes observed in dogs with leishmaniasis, occurs largely because of vasculitis, which itself results from the deposition of circulating immune complexes on vessel walls (BRITO et al, 2010). Hence, uveitis in dogs with VL has an immunological source (PEÑA et al, 2000).…”

Section: Resultsmentioning

confidence: 99%

“…These synechiae were probably to the result of the direct action of amastigote forms of the parasite or of the deposition of immune complexes on the anterior uvea (BRITO et al, 2010).…”

Section: Resultsmentioning

confidence: 99%

“…It is important to note that we found no inflammatory process of the posterior segment in any of the 16 eyes (eight dogs) that underwent histopathological examinations. However, dogs with VL may present lesions in the posterior segment, such as chorioretinitis and retinal detachment (CIARAMELLA et al, 1997;PEÑA et al, 2000;BRITO et al, 2010) as a result of the expansion of the inflammatory processes from the anterior segment (COLLINS and MOORE, 1991).…”

Section: Resultsmentioning

confidence: 99%

“…CVL conjunctivitis occurs mainly as a result of the deposition of circulating immune complexes, which triggers vasculitis (BRITO et al, 2010). Another mechanism that may be involved in its development is the direct action of the parasite upon the conjunctiva (FULGÊNCIO, 2006;BRITO et al, 2010).…”

Section: Resultsmentioning

confidence: 99%

“…This ocular lesion can occur in dogs with VL (PEÑA et al, 2008) and likely arises from the deposition of immune complexes (BRITO et al, 2010).…”

Section: Resultsmentioning

confidence: 99%

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Ocular diseases in dogs naturally affected by visceral leishmaniasis in Teresina, Piauí, Brazil

et al. 2017

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This study aimed to characterize ocular diseases in dogs naturally affected by visceral leishmaniasis in Teresina, Piauí State, Brazil. The diagnosis was made using parasitological exams of the bone-marrow and lymph-node samples. The main ophthalmological findings were uveitis, conjunctivitis, blepharitis, keratitis, and keratoconjunctivitis sicca. Normocytic normochromic anemia was the main hematological finding, followed by thrombocytopenia. Plasma proteins were also considered, and hyperproteinemia, hypergammaglobulinemia, and hypoalbuminemia were observed. Ocular histopathological examination revealed mild inflammation involving lymphocytes, monocytes, and macrophages. Results indicate the need to perform a differential diagnosis to rule out or establish the presence of Leishmania sp. in dogs presenting with ophthalmic lesions in endemic regions.

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Section: Resultsmentioning

confidence: 99%

“…These synechiae were probably to the result of the direct action of amastigote forms of the parasite or of the deposition of immune complexes on the anterior uvea (BRITO et al, 2010).…”

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

“…This ocular lesion can occur in dogs with VL (PEÑA et al, 2008) and likely arises from the deposition of immune complexes (BRITO et al, 2010).…”

Section: Resultsmentioning

confidence: 99%

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Ocular diseases in dogs naturally affected by visceral leishmaniasis in Teresina, Piauí, Brazil

et al. 2017

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Study of ocular manifestations and humoral immune response in eyes of dogs with leishmaniasis

Goulli

Zribi

Sanhaji

et al. 2022

Veterinary Medicine & Sci

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Background Ocular manifestations in dogs with leishmaniasis are frequent and complications in affected tissues can lead to blindness. Immune processes play a very important role in the pathogenesis of ocular inflammation. Therefore, the immunology of ocular manifestations in dogs with leishmaniasis remains complex and poorly understood. Objectives Estimation and characterisation of ocular and periocular manifestations in dogs naturally infected with Leishmania infantum and investigation of the production site of specific anti‐Leishmania infantum IgG. Methods The present investigation used 53 confirmed dogs infected with Leishmania infantum, presenting ocular and periocular lesions, and 10 control non‐infected dogs. Complete macroscopic ophthalmic examination of eyelids and globes was performed. Both total and anti‐Leishmania infantum IgG antibodies were studied in sera and aqueous humour (AH) of all dogs by ELISA technique. A Goldmann–Witmer coefficient (C value) was calculated. Results The main ophthalmological findings were keratoconjunctivitis (71.7%; 38/53), hyperplasia of conjunctival lymphoid follicles (54.7%; 29/53), blepharitis (50.9%; 27/53) and uveitis (20.7%; 11/53). Ocular production of anti‐Leishmania infantum IgG was detected in 73.6% (39/53) of infected dogs. There was no correlation between the antibody levels in AH and sera of the same dog. The mean anti‐Leishmania infantum IgG in AH was higher in uveitis, followed by lesions affecting only the adnexa (p < 0.0001). The highest mean C values were observed for uveitis, conjunctivitis and keratitis. Conclusions Our findings suggest that production of anti‐Leishmania IgG in dogs infected with Leishmania infantum with ocular manifestations begin in situ and follows by a transfer of antibodies from the bloodstream to the AH.

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Canine leishmaniasis in an endemic region, Northeastern Brazil: a comparative study with four groups of animals

et al. 2021

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Canine leishmaniasis (CanL) is a zoonosis caused by protozoa of the genus Leishmania and remains an important public health concern in tropical areas. In Brazil, domestic dogs are considered the most relevant reservoir of the parasite and one of the main targets of the disease control actions. Considering this, we aimed herein to evaluate the CanL infection in different canine groups and distribution of cases in the state of Sergipe, an endemic region in Northeastern Brazil. The evaluated 467 animals were classified into four groups: hunting ( n = 50), company ( n = 64), guard ( n = 140), and wandering ( n = 213). Samples (blood, bone marrow, conjunctival swab, and lymph node aspirate) were collected from animals in nine municipalities of Sergipe. First, all animals were submitted to general and ophthalmic clinical examination. Next, they were tested serologically by TR-DPP®, and for the presence of Leishmania , amastigotes in samples of bone marrow, conjunctival swab, and lymph node aspirate were diagnosed by PCR and parasitological techniques. It was observed that 34.69% (162) of the evaluated dogs were seropositive. The highest rates of positivity were found in hunting 54% (27/50; OR = 3.52; p -value = 0.001) and guard dogs 42.14% (59/140; OR = 2.18; p -value = 0.01). Otherwise, the highest percentage of symptomatic dogs was observed in wandering animals (85%; OR = 9.63; p -value < 0.0001). The distribution of case analysis showed that the highest positivity rates occurred in inland municipalities situated in arid regions. Taken together, our data demonstrate that hunting and guard dogs are among the animals most exposed and affected by clinical manifestations of CanL, mainly in the inland municipalities of Sergipe State. Supplementary Information The online version contains supplementary material available at 10.1007/s00436-021-07319-0.

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Histopathological findings and detection of parasites in the eyes of dogs infected naturally with Leishmania chagasi

Abstract: The objective of the present study was to investigate the histopathological alterations and detection of parasites that occur in the eyes of dogs naturally infected with

Cited by 11 publications

References 16 publications

Ocular diseases in dogs naturally affected by visceral leishmaniasis in Teresina, Piauí, Brazil

Ocular diseases in dogs naturally affected by visceral leishmaniasis in Teresina, Piauí, Brazil

Study of ocular manifestations and humoral immune response in eyes of dogs with leishmaniasis

Canine leishmaniasis in an endemic region, Northeastern Brazil: a comparative study with four groups of animals

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