Histologic and Histometric Examination of Spleen in Geese (Anser anser)

Abstract

Abstract:The aim of this study was to examine the histometrical and histological structures of goose (Anser anser) spleen.Six healthy female geese were used as material. Tissue samples taken from the spleen were processed routinely, and were then stained with H&E, Crossman's Triple stain and Toluidine blue stain. The spleen surrounded by capsules composed of connective tissue and parts of the capsules were observed increasingly thinning into the spleen as trabeculae. The red pulp area was distinguishable from the white pulp inside the organ; further, the lymph follicles appeared clearly within the white pulp. Histometric measurements revealed that the thickness of the capsules surrounding the organ ranged from 18 to 28 µm. The average thickness of the capsules was measured as 22 µm. The average number of lymph follicles was found to be 2.4 in 1.07 mm 2 . The average width and length of the lymph follicles were measured as 113 µm and 144 µm, respectively.The average diameter of the mast cells was found to be 6 µm. The average number of mast cells was found to be 1.4 in 1.07 mm 2 . Although the histological structures of the geese spleens seemed very similar to those of other animals in several respects, but some specific properties of geese spleens being more similar to that of mammalians were also observed.

Structure of the Eurasian moorhen spleen: A comprehensive study using gross anatomy, light, and transmission electron microscopy

“…Similar findings were reported in quail (Liman & Bayram, 2011) and dove (Nasu, Shimizu, & Nakai, 1992). On the other hand, our findings are not in accordance with those of Sultana, Khan, Wares, and Masum (2011) in duck and Bingöl, Gülmez, Deprem, Taşci, and Aslan (2014) in geese which reported the presence of numerous trabeculae in spleens of abovementioned species. As the blood storage and release capacity of the spleen is positively correlated with the thickness of its capsule and degree of development of the trabeculae (Hartwig & Hartwig, 1985; Udroiu, 2007), the ability of moorhen's spleen to store blood is poor.…”

“…Both trabecular arteries and veins were observed within the poorly developed trabeculae of moorhen's spleen. Similar observations were seen in the spleens of quail (Kadam, Waghaye, & Thakur, 2019) and geese (Bingöl et al, 2014). The trabecular veins drain blood from red pulp sinusoids and pass it to the splenic vein.…”

Architecture and Cellular Composition of the Spleen in the Japanese Quail (Coturnix japonica)

“…The current study revealed no clear demarcation between white pulp and red pulp, and similar results were observed in the spleen of chicken and dove (Nasu et al, 1992; John, 1994; Baishya & Bhattacharyya, 2012; Kannan & Ramesh, 2015). However, El-Dawi (2008)) and Bingöl et al (2014) observed a clear line of demarcation between the white and red pulp in chicken and geese spleen. This is in contrast to mammalian spleen which showed a clear marginal zone and a clear line of demarcation between the white and red pulp of the spleen (Roitt et al, 1989; Tanaka, 1990).…”

Morphohistometric Evaluation of Embryonic Development of Spleen in Chicken

“…There is no real trabecular structure in the winged spleen wrapped with a thin capsule (Olah and Vervelde 2008, Liman and Bayram 2011, Bingöl et al 2014, Khenenou et al 2018. Song et al (2012) reported that the spleen membrane thickness in ostriches was 25.041.5 µm and undeveloped spleen trabeculae were encountered.…”