Morphohistometric Evaluation of Embryonic Development of Spleen in Chicken

Abstract

The aim of this study was to evaluate the morphohistometric development of chick spleen by considering specific embryonic periods. For the study, spleens obtained from 18 Babcock White Leghorn chick embryos on the 13 th , 16 th and 21 st days of incubation were used. The sections were stained with Crossmon's trichrome stain and Pappenheim's panoptic stain and differential leukocyte counts were made in the blood smears. In the measurements, an increase in the spleen volume, embryo weight and vitellus sac weight were determined. There was an increase between the 13 th and 16 th 21 st days in spleen volume. The highest heterophil granulocytes (74.83%) and lowest lymphocyte ratio (23%) were found on the 21 st day. On the 13 th day, there were very few lymphocytes around the vessels. On the 16 th day, arteria centralis were frequently encountered and periarteriolar lymphoid tissue formation with lymphocyte accumulations around them started to develop in the spleen parenchyma. The red and white pulp areas could be easily distinguished in splenic parenchyma on the 21 st day. It was concluded that the structures characterised by lymphocyte infiltrations in the spleen parenchyma were formed and caused changes in the number of lymphocytes in peripheral blood during the embryonal period.

Effects of Sunset Yellow FCF on immune system organs during different chicken embryonic periods

“…The spleens were separately weighed using a precision balance and the weights noted. In the histological examination of spleen sections, the average capsule thickness was obtained by measuring six different regions with the analysis programme ( 9 ). Spleen volumes were calculated using a point area measurement scale (d = 0.2 mm) on the cross-sectional images taken at 4× lens magnification and Cavalieri’s principle was applied as the calculation method ( Fig.…”

Mir-140 and Mir-34a as Molecular Markers for Apoptotic Brain in Sunset Yellow and Carmoisine Intoxicated Mice

“…Sunset yellow (SY) has been listed by National Institute for Occupational Safety and Health (NIOSH) as having behavioural consequences involving coma with impact on seizure threshold only in acute high doses (LD50) of SY [8]. SY was reported to have mutagenic and carcinogenic [9], immunological [10][11][12] effects in mammals. Car could alter the different bio-elements levels in liver, kidney and brain [13], trigger 38 renal failure, hepatotoxicity [14] combined with genotoxicity [15] .…”