The processes of digestion in the avian gastrointestinal tract depend on sophisticated control systems that co-ordinate secretion of digestive juices and movement of the luminal contents. In the current study, the distribution of serotonin-, gastrin-, glucagon-and somatostatin-immunoreactive endocrine cells was investigated by immunocytochemical methods in the intestinal tract of the goose. The number of cells immunoreactive for each antiserum was evaluated in different regions of the intestinal tract. Serotonin-, glucagon-and somatostatinimmunoreactive endocrine cells were seen throughout the intestinal tract, but somatostatin-immunoreactive cells were not detected in the colon of the goose. Gastrin-immunoreactive cells were detected only in the duodenum, jejunum and colon mucosa. It is concluded that the distribution pattern of the entero-endocrine cells in the goose is similar to that of most of the mammalian and other poultry species.
The objective of the present study was to determine the effects of follistatin addition on myostatin and follistatin gene expression patterns in C 2 C 12 muscle cells. C 2 C 12 cells were administered with 100 ng/ml recombinant human (rh) follistatin in Dulbecco's modified Eagle medium (DMEM) containing 10% fetal bovine serum (FBS), 4 mM glutamine and antibiotics daily for three days. Rh follistatin was not added in the control wells. Follistatin and myostatin gene cDNAs were synthesised by reverse transcriptase polymerase chain reaction (RT-PCR). The time course of follistatin gene expression pattern was similar in both the control and the follistatin-treated group. Myostatin mRNA level significantly increased in the follistatin-treated group after 24 h of culture ( Fig. 3, P < 0.01). Amounts then sharply decreased (Fig. 3, P < 0.01) at 48 h of culture, whereas there was no significant difference between the control and the follistatin-treated group at 72 h of culture. Our results demonstrated that myostatin and follistatin mRNA were expressed in C 2 C 12 cells and rh follistatin changed the myostatin expression pattern.
Pancreas in the vertebrates is subdivided into two parts: One of them is exocrine part where digestive enzymes are released and the other one is endocrine part where regulatory hormones are released into blood circulation 1 . The endocrine part, formed by the islets of Langerhans, is multihormonal unit composed of at least four types of cells; the insulin (B cells), the glucagon (A cells), the somatostatin (D cells), and the pancreatic polypeptide (PP cells) 2 .Insulin is synthesized in the B cells of the pancreatic islets and regulates the blood glucose levels. Glucagon
Abstract:The aim of this study was to examine the histometrical and histological structures of goose (Anser anser) spleen.Six healthy female geese were used as material. Tissue samples taken from the spleen were processed routinely, and were then stained with H&E, Crossman's Triple stain and Toluidine blue stain. The spleen surrounded by capsules composed of connective tissue and parts of the capsules were observed increasingly thinning into the spleen as trabeculae. The red pulp area was distinguishable from the white pulp inside the organ; further, the lymph follicles appeared clearly within the white pulp. Histometric measurements revealed that the thickness of the capsules surrounding the organ ranged from 18 to 28 µm. The average thickness of the capsules was measured as 22 µm. The average number of lymph follicles was found to be 2.4 in 1.07 mm 2 . The average width and length of the lymph follicles were measured as 113 µm and 144 µm, respectively.The average diameter of the mast cells was found to be 6 µm. The average number of mast cells was found to be 1.4 in 1.07 mm 2 . Although the histological structures of the geese spleens seemed very similar to those of other animals in several respects, but some specific properties of geese spleens being more similar to that of mammalians were also observed.
The experimental applications performed on mice were carried out with approval from the Kafkas University Animal Experiments Local Ethics Committee (04.04.2007/13).Thirty-six 8-to 12-week-old male Swiss albino mice were used. Mice used in the experiment were divided into 3 groups as experimental (diabetic) (n = 15), sham (n = 15), and control (n = 6). A single dose of 100 mg/kg (9) STZ
Probiotics are currently under investing the most valuable substances alternative to antibiotic growth promoters in poultry breeding practice. This research was performed to evaluate the effect of supplementing broiler drinking water with probiotics (Pediococcus acidilactici and Bacillus subtilis) at a concentration of ≥10 8 CFU/ml during 42 days of feeding period on growth performance and gut health. A total of 144 one-day-old Ross 308 broiler chicks (mixed gender) with an average initial BW of 42.3 g were used. The chicks were allotted to pens with 12 birds per pen and six replications per treatment with food and water provided ad libitum. Feed intake of Probiotic group was 4134 g, 338 g less than that of control group. Live weight of probiotic group was 2537 g and a 113 g more than that of control group. The feed conversion ratio of probiotic group was 1.61, 0.22 less than that of control group. The crypt depth of probiotic group (1110.46 ± 224.016 µm) was statistically deeper than that of control group (949.39 ± 114.166 µm) in ileum. Continuously use of probiotics in drinking water of commercial poultry flocks appears to be alternative to AGPs. The results of this study provide a greater understanding of the impact of long-life use of probiotics on broiler health and growth performances.
Alpha-lipoic acid (ALA) is extensively utilized in multivitamin formulas and anti-aging products. The purpose of this study was to investigate the potential protective benefits of ALA on 5-fluorouracil (5-FU)-induced gastrointestinal mucositis in Wistar albino rats. Tissues from the stomach, small intestine, and large intestine were excised, and blood sera were obtained to identify biochemical indices such as TNF-α, IL-1β, MDA, GPx, SOD, MMP-1, -2, -8, and TIMP-1. A histopathological study was also performed. The results revealed mucositis-elevated TNF-, IL-1, MDA, MMP-1, -2, -8, and TIMP-1 levels in both tissues and sera, and these values dropped dramatically following ALA treatment. Reduced SOD and GPx activities in mucositis groups were reversed in ALA-treated groups. The damage produced by mucositis in the stomach and small intestine regressed in the ALA-treated group, according to histopathological evaluation. Consequently, the implementation of ALA supplementation in 5-FU therapy may act as a protective intervention for cancer patients with gastrointestinal mucositis. In light of the findings, ALA, a food-derived antioxidant with pleiotropic properties, may be an effective treatment for 5-FU-induced gastrointestinal mucositus, and prevent oxidative stress, inflammation, and tissue damage in cancer patients receiving 5-FU therapy.
The myocardial bridge (MB) is an anomaly that the myocardial fibres cover on a segment of the subepicardial coronary arteries or their branches in domestic animals and humans. The aim of the present study was to determine the relationships between the characteristics of the MB and ramus interventricularis paraconalis at three levels in lambs and adult sheep. Thirty‐three hearts (16 lambs and 17 sheep) were used to determine the MB (length, angle and thickness) and vessel (vessel diameter and thicknesses of tunica intima et media of ramus interventricularis paraconalis) characteristics. Independent‐samples t test was applied to compare variables between lambs and sheep. Spearman's correlation analysis was conducted to evaluate the relationships between bridge and vessel characteristics at three bridge levels. Length, angle and thickness of myocardial bridges were not significantly different between the lambs and sheep (p > .05). The mean length, angle and thickness were 24.9 ± 16.1 mm, 113.7 ± 11.2° and 1,098 ± 555 µm in 33 hearts, respectively. In lambs, the mean vessel diameters were 1,930 ± 742 µm (1,534–2,325 µm), 1,247 ± 665 µm (893–1,601 µm) and 865 ± 172 µm (774–957 µm) at the pre‐bridge, bridge and post‐bridge levels, respectively. In sheep, the mean vessel diameters in the same order were 1,861 ± 1,068 µm, 1,337 ± 308 µm and 1,287 ± 549 µm. The bridge prevalence was 100% in the samples examined. In conclusion, coronary arterial diseases related to myocardial bridge should not be expected in sheep for veterinary cardiology practice. It may also be concluded that the cross‐breeds of the Awassi and Chios sheep may be useful in experimental studies related to myocardial bridge surgery.
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