Highly Sensitive Spectrofluorimetric Method for Determination of Certain Aminoglycosides in Pharmaceutical Formulations and Human Plasma

Abstract

A simple, reliable, highly sensitive and selective spectrofluorimetric method has been developed for determination of certain aminoglycosides namely amikacin sulfate, tobramycin, neomycin sulfate, gentamicin sulfate, kanamycin sulfate and streptomycin sulfate. The method is based on the formation of a charge transfer complexes between these drugs and safranin in buffer solution of pH 8. The formed complexes were quantitatively extracted with chloroform under the optimized experimental conditions. These complexes showed an excitation maxima at 519-524 nm and emission maxima at 545-570 nm. The calibration plots were constructed over the range of 4-60 pg mL(-1) for amikacin, 4-50 pg mL(-1) for gentamicin, neomycin and kanamycin, 4-40 pg mL(-1) for streptomycin and 5-50 pg mL(-1) for tobramycin. The proposed method was successfully applied to the analysis of the cited drugs in dosage forms. The proposed method was validated according to ICH and USP guidelines with respect to specificity, linearity, accuracy, precision and robustness. The high sensitivity of the proposed method allowed determination of amikacin and gentamicin in spiked and real human plasma.

Utility of 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole for development of a highly sensitive stability indicating spectrofluorimetric method for determination of salmeterol xinafoate; application to human plasma

“…On the other hand, spectrouorimetric methods have the advantages of being simple, highly sensitive and time saving technique. [12][13][14][15][16] To our knowledge, there is no reported spectrouorimetric method for determination of salmeterol xinafoate up till now. In this study, we investigate the reaction of salmeterol xinafoate that contains a basic secondary amine with NBD-Cl which has been widely used for the determination of many pharmaceuticals containing primary and secondary amino groups [17][18][19][20][21][22] in order to develop new, simple, sensitive and rapid spectrouorimetric method for determination of the studied drug in its dosage form, spiked plasma and in the presence of its degradation products.…”

Micellar enhanced spectrofluorimetric approach for nanogram detection of certain α1‐blocker drugs: Application in pharmaceutical preparations and human plasma

“…% recovery in vitro is % recovery for drug in spiked human sample and delivery in vitro is the concentration of the drug in spiked human sample . All results were summarized in Table .…”

Penetapan kadar gentamisin dalam sediaan krim dengan kromatografi lapis tipis - densitometri

“…Metode ini juga dilaporkan untuk penetapan kadar gentamisin (kombinasi dengan meropenem) dalam serum dan plasma (Zuzanna et al, 2006). Analisis kuantitatif gentamisin terutama untuk tujuan pengendalian mutu sediaan telah dikembangkan dengan berbagai metode, misalnya kromatografi kinerja tinggi (KCKT) (Isoherranen, 2000;Kuehl, 2010), dan Omar et al (2013) juga melaporkan metode spektrofotometri untuk penetapan kadar gentamisin dalam calam campurannya dengan vankomisin, namun membutuhkan tahapan yang tidak sederhana (Clarot et al, 2004;El-Didamony et al, 2006;Gubernator et al, 2006).…”