Micellar enhanced spectrofluorimetric approach for nanogram detection of certain α₁‐blocker drugs: Application in pharmaceutical preparations and human plasma

Abstract

Alpha1-adrenergic-blocking drugs, namely; alfuzosin hydrochloride (ALF), doxazosin mesylate (DOX) and terazosin hydrochloride (TER) are effective as antihypertensive agents as well as in management of benign prostatic hypertrophy. In this study, a simple, very fast, highly sensitive and cheap technique was optimized for assay of these drugs in pure states and pharmaceutical tablets. The proposed method is dependent on enhancement of the native fluorescence of investigated drugs using the polyoxyethylene 50 stearate (POE50S) micellar system. The method showed excitation at 325, 340 and 250 nm for ALF, DOX and TER, respectively and an emission maxima at 382 nm. The fluorescence intensity-concentration charts of studied drugs were attained utilizing concentration ranges (2.0-60.0 ng mL ) for DOX and (4.0-100.0 ng mL ) for ALF and TER with quantitation limits 2.9, 1.6 and 2.5 ng mL for ALF, DOX and TER, respectively. The suggested technique was approved according to International Council for Harmonisation (ICH) standards and the United States Food and Drug Administration (US FDA) bioanalytical method validation and has been effectively applied for assay of these medications in their dosage forms as well as for content uniformity test. The developed procedure was also efficiently applied for determination of these drugs in real human plasma with high accuracy.

Development of sensitive benzofurazan‐based spectrometric methods for analysis of spectinomycin in vials and human biological samples

“…Each 0.5 ml of plasma was transferred to a centrifugation tube and spiked with various aliquots of the standard SPRC solution. Here, 1 ml acetonitrile was utilized as a protein precipitation agent, [17][18][19][20] the content was completed to 10 ml with distilled water and then centrifuged at 18 659 g for 10 min. The general analytical procedure was performed exactly as described in Section 2.4.…”

Utility of the fluorogenic characters of benzofurazan for analysis of tigecycline using spectrometric technique; application to pharmacokinetic study, urine and pharmaceutical formulations

“…Acetonitrile was added (2 mL) as a protein precipitating agent. [10][11][12] The resultant supernatant solution was completed to 10 mL with distilled water and then centrifugation at 4000 rpm for 20 min. Furthermore, 2 mL of the supernatant was used to provide the final concentration range (40-500 ng mL −1 ) in the general procedure.…”

A new green fluorimetric micelle complexation approach for reduction of the consumed solvent and quantification of avapritinib in biological fluids

“…The molar absorptivity and/or uorescence quantum yield of a given uorophore solution frequently increases when a surfactant is added at a concentration greater than its critical micellar concentration. [14][15][16] This technique has been used for the direct estimation of the anticancer drug AVA in biological uids. This method has been applied to uniformity tests, pharmacokinetic studies, and greenness assessments.…”

“…The molar absorptivity and/or fluorescence quantum yield of a given fluorophore solution frequently increases when a surfactant is added at a concentration greater than its critical micellar concentration. 14–16…”