Utility of 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole for development of a highly sensitive stability indicating spectrofluorimetric method for determination of salmeterol xinafoate; application to human plasma

Omar, Mahmoud A.; Hammad, Mohamed A.; Awad, Mohamed A.

doi:10.1039/c7ra08106e

Cited by 18 publications

(16 citation statements)

References 20 publications

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“…NBD-Cl was previously used as a fluorogenic reagent for determination of many pharmaceutical drugs having primary or secondary amine moiety at mild alkaline medium producing fluorogenic products. [15][16][17][18] In this work, the reaction of secondary amine moiety in TIGE with NBD-Cl was achieved using borate buffer at pH 9 ± 0.2 at 75°C after heating for 15 min. The proposed method showed higher sensitivity than the previously reported spectrofluorimetric methods.…”

Section: Resultsmentioning

confidence: 99%

Utility of the fluorogenic characters of benzofurazan for analysis of tigecycline using spectrometric technique; application to pharmacokinetic study, urine and pharmaceutical formulations

et al. 2019

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Tigecycline (TIGE) is the newest tetracycline derivative antibiotic with low toxicity, it is used for management of infectious diseases caused by Gram-positive and Gramnegative bacteria. Hence, an efficient, selective and sensitive method was developed for analysis of TIGE in commercial formulations, human plasma and urine. The spectrofluorimetric technique based on the reaction of secondary amine moiety in TIGE with 4-chloro-7-nitrobenzofurazan (NBD-Cl) in slightly alkaline medium producing a highly fluorescent product measured at 540 nm (λ ex at 470 nm) after heating for 15 min at 75°C. The proposed strategy was upgraded and approved by ICH rules and bio-analytical validated using US-FDA recommendations. A linear relationship between fluorescence intensity and TIGE concentration was observed over the concentration range 40-500 ng mL −1 with limit of quantification (LOQ) 21.09 ng mL −1 and limit of detection (LOD) 6.96 ng mL −1 .The ultra-affectability and high selectivity of the proposed strategy permits analysis of TIGE in dosage form, human plasma and urine samples with good recovery ranged from 97.23% to 98.72% and from 99.36% to 99.80% respectively, without any interfering from matrix components. Also, the developed strategy was used to examine the stability of TIGE in human plasma and applied for pharmacokinetic investigation of TIGE.

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Section: Resultsmentioning

confidence: 99%

Utility of the fluorogenic characters of benzofurazan for analysis of tigecycline using spectrometric technique; application to pharmacokinetic study, urine and pharmaceutical formulations

et al. 2019

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“…[23,26] Expanding the pH range of the 0.1 M borate buffer to values higher than 9.5 caused a decrease in relative fluorescence intensity (RFI) due to increase in hydroxide group concentration that prevented the reaction between SPEC and NBD-Cl; this result concurred with those of previously published studies. [12,23,26] FIGURE 2 (a) Absorption and (b) excitation (460 nm) and emission (530 nm) spectra of the reaction product formed between NBD-Cl and SPEC (2 μg ml −1 and 150 ng ml −1 respectively)…”

Section: Effect Of Phmentioning

confidence: 99%

“…[26] The addition of 1.0 ± 0.1 ml of 2 M HCl was required to quench the NBD-OH fluorescence, as shown in Figure S2, without changing the fluorescence of the yielded product. [12,23,26]…”

Section: Effect Of Volume Of Hclmentioning

confidence: 99%

“…[1,[6][7][8] 4-Chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) is a chemical reagent that reacts with amine moietycontaining drugs forming fluorogenic and chromogenic products under alkaline conditions. [12][13][14][15] The methods presented here represent the first use of NBD-Cl to derivatize SPEC, firstly measuring the product spectrophotometrically at 410 nm and secondly measuring the product spectrofluorimetrically at 530 nm (λ ex at 460 nm). The aim of this study was to establish a highly sensitive, simple, low-cost and selective method to determine SPEC both in vials and in biological samples with a high percentage recovery and without any interference from the matrix solution.…”

Section: Introductionmentioning

confidence: 99%

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Development of sensitive benzofurazan‐based spectrometric methods for analysis of spectinomycin in vials and human biological samples

et al. 2019

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Spectinomycin hydrochloride (SPEC) is an aminoglycoside antibiotic that has a broad spectrum against several bacterial strains of humans and veterinary infections.However, SPEC lacks a fluorophore or chromophore and this lack makes its analysis a challenge. Our study provides a simple, sensitive and low-cost spectrofluorimetric/spectrophotometric method based on the reaction between secondary amine groups and a benzofurazan reagent using borate buffer (pH 9.2). The yielding compound was measured fluorimetrically at 530 nm (excitation at 460 nm) with colorimetry at 410 nm. The calibration curves ranged from 30 to 400 ng ml −1 and from 0.2 to 6 μg ml −1 for spectrofluorimetric and spectrophotometric analyses, respectively. The limits of detection were calculated to be 4.15 ng ml −1 and 0.05 μg ml −1 for spectrofluorimetric and spectrophotometric processes, respectively.The ultra-affectability and high selectivity of the proposed method permitted analysis of SPEC in the dosage form and in human plasma samples, with good recoveries of about 101.19 and 97.11%, respectively, without any matrix interference. The proposed strategy was validated using International Conference on Harmonization standards and validated bioanalytically using USFDA recommendations.

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“…Accordingly, it needs to be derivatized first with a fluorogenic reagent to enhance the sensitivity required for its bioanalysis. 4‐Chloro‐7‐nitrobenzofurazan (NBD‐Cl) is a well known, stable, nonfluorescent reagent that is utilized frequently as a derivatizing agent for quantification of several drugs containing the amino group . In the current study, NBD‐Cl has been selected as it is very reactive under mild reaction conditions, has a long shelf life, is available in any analytical laboratory, and is cheap and highly photostable compared with other existing derivatizing reagent such as fluorescamine and dansyl chloride .…”

Section: Introductionmentioning

confidence: 99%

Novel spectrofluorimetric quantification of alogliptin benzoate in biofluids exploiting its interaction with 4‐chloro‐7‐nitrobenzofurazan

Aref¹,

Hammad

Darwish

et al. 2019

Luminescence

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The direct determination of alogliptin benzoate (ALO) using fluorescence has not yet been accomplished because ALO cannot fluoresce naturally. Accordingly, it should be derivatized first with a fluorogenic reagent to enhance the sensitivity required for its bioanalysis. This method is the first spectrofluorimetric assay for ALO quantification exploiting the nucleophilic nature of its amino group to react with 4‐chloro‐7‐nitrobenzofurazan (NBD‐Cl) in borate buffer at pH 8.5 to produce a strong fluorescent compound that is excited at and emits at wavelengths 470 and 527 nm, respectively. Experimental variables concerning the conditions of reaction and fluorogenic intensity were carefully investigated and optimized. Linearity was from 1–250 ng ml−1 with a lower detection limit of 0.29 ng ml−1 and a lower quantification limit of 0.88 ng ml−1. Validation of the current study was accomplished with mean per cent recovery of 100.62 ± 1.59 in tablets and 99.86 ± 0.82 in human plasma. Furthermore, the current method has been utilized in the bioanalysis of ALO in real rat plasma after oral administration with a simple specimen preparation. The developed method has proven to be a promising alternative method for ALO analysis in bioequivalence studies.

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Utility of 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole for development of a highly sensitive stability indicating spectrofluorimetric method for determination of salmeterol xinafoate; application to human plasma

Abstract: Suggested reaction mechanism between salmeterol xinafoate and NBD-Cl.

Cited by 18 publications

References 20 publications

Utility of the fluorogenic characters of benzofurazan for analysis of tigecycline using spectrometric technique; application to pharmacokinetic study, urine and pharmaceutical formulations

Utility of the fluorogenic characters of benzofurazan for analysis of tigecycline using spectrometric technique; application to pharmacokinetic study, urine and pharmaceutical formulations

Development of sensitive benzofurazan‐based spectrometric methods for analysis of spectinomycin in vials and human biological samples

Novel spectrofluorimetric quantification of alogliptin benzoate in biofluids exploiting its interaction with 4‐chloro‐7‐nitrobenzofurazan

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