High Efficiency in vitro Plant Regeneration and Secondary Metabolite Quantification from Leaf Explants of Rhodiola imbricata

Abstract

4This stress condition helps in increasing secondary metabolite production. 5 These plants have been used for medico-religious purposes which in turn help in generating income for local people.6 Out of several medicinal plants found in trans-Himalayas, Rhodiola imbricata frames to be a distinctive herb due to the presence of various phytopharmaceutical constituents which can be helpful for human kind. High Efficiency in vitro Plant Regeneration and Secondary Metabolite Quantification from Leaf Explants of Rhodiola imbricataABSTRACT Aim: Rhodiola imbricata is an endangered medicinal plant of the trans-Himalayan Leh-Ladakh region belonging to the family Crassulaceae. An efficient propagation and regeneration system via direct shoot organogenesis from leaf explant and evaluation of cinnamyl alcohol (Secondary metabolite) was established in this study. Material and Methods: In vitro grown leaves were inoculated using Murashige and Skoog (MS) medium supplemented with (alpha)-naphtalene acetic acid (NAA) in combination with 6-benzyladenine (BAP) for callus proliferation and regeneration. Results: The highest percentage of rhizogenous callus was induced in medium containing NAA (10.0-15.0 µM). The highest percentage of shoot formation from leaf derived callus was obtained in the medium containing of NAA (5.0 µM) and BAP (2.5 µM) as well as in NAA (1.0 µM), BAP (5.0 µM) (38.88% and 37.49%) respectively. Rooting of regenerated shoots were effective when a lower concentration of NAA (0.5 μM) was used alone. A maximum number of roots (22.0) and higher length (0.6 cm) was observed. The in-vitro plantlets with well-developed shoots and roots were acclimatized successfully to natural field conditions with a survival rate of over 80%. Cinnamyl alcohol (Secondary metabolite) evaluation was also done for the very first time and an upregulation of 49.6% and 30.6% were observed in in-vitro roots and shoots when compared with mother root and shoot respectively. Hence, it was proved that the content of secondary metabolites obtained from in-vitro raised plants is higher than mother plant. Conclusion : These results may lay a foundation for genetic improvement and can be used to determine sequential level of targeted secondary metabolites through cell culture in Rhodiola imbricata.

Regeneration and Genetic Transformation by pAB6 Plasmid of Hyoscyamus muticus L., (Egyptian Henbane) Using Particle Bombardment

“…These results agree with those of Abd- Elmaksood et al, (2016), who proposed MS medium supplemented with 1.0 mg L -1 KN as the most promising treatment for establishment and continuous proliferation of H. muticus L. explants during successive subcultures. Ali et al, (2010) studied media types, strengths, components and combinations of auxin and cytokinin (NAA and BA) and found that the light and inoculum density increased total alkaloid which accumulated in suspension cultures of Hyoscyamus muticus L. The same pattern was reported by Bhardwaj et al (2018), who observed high percentage of callus induction in medium containing 10 to 15 µM NAA from Rhodiolaimbricata genotypes with highest percentage of shoot given by leaf derived callus medium of 5 µM NAA and 2.5 µM BAP as well as1.0 µM NAA , 5 µM BAP, 5 µM (38.88% and 37.49%); rooting of regenerated shoots as effective when a lower concentration of NAA (0.5 µM) was used alone. However, a maximum number of roots (22.0) and higher length (0.6 cm) was observed.…”

GC–MS analysis of chemical constituents and determination of the total antioxidant capacity of adult powder of Periplaneta americana

“…Besides that, the presence of 1-Hentetracontanol has also been found in some medicinal plants with medicinal values such as Kigelia Africana (Lam.) (Fagbohun et al 2020), Rhodiola (Bhardwaj et al 2018), Araucaria columnaris (Patial & Cannoo 2021). D-Mannitol, is an important essential medicine in the World Health Organization (WHO) list of important medicines.…”

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“…The in-vitro propagation technique is found to be an alternative method because it offers a supply of biochemical independent of season and availability of plants 7,8,9 . Earlier, there have been few reports on the establishment of in-vitro propagation through nodal explants of Cleome gynandra 10 . The present communication deals with the development of a rapid regeneration system for indirect organogenesis through leaf explants of Cleome gynandra.…”