Hematological and immunological responses of Nile tilapia after polyvalent vaccine administration by different routes

Abstract

7 formalin-inactivated bacteria·mL -1 , for 20 minutes. Vaccinated fish groups presented higher hematocrit, number of erythrocytes and leukocytes than the non-vaccinated group. Serum agglutination titer of intraperitoneally vaccinated fish was higher on both evaluation periods for the three bacteria strains. Only on day 21 post-vaccination fish from the oral and immersion vaccination groups presented higher serum agglutination titer than the non-vaccinated fish for A. hidrophyla and E. durans. Serum antimicrobial activity in vaccinated fish was higher for P. aeroginosa and E. coli than in non-vaccinated fish on both evaluation periods. The different vaccine administration routes stimulated hematological and immunological responses in Nile tilapia 21 days post-vaccination, but intraperitoneal vaccination presented higher total number of leukocytes, lymphocytes and serum agglutination titer.

Probiotic supplementation in diet and vaccination of hybrid surubim (Pseudoplatystoma reticulatum♀ x P. corruscans♂)

“…The lymphocyte number in blood from the two vaccinated groups was lower when compared to unvaccinated fish (Table 1). Comparing with other studies, intraperitoneal vaccination in Nile tilapia (O. niloticus) led to an increase in lymphocyte number, after 7 and 21 days post-vaccination, and also after 21 days when the fish were orally vaccinated (SILVA et al, 2009). In this case, the decrease of lymphocytes could be due to their migration to the tissues as a response to the vaccination.…”

“…The title serum agglutination as well as the blood serum antimicrobial activity were prepared according methodology described by SILVA et al (2009). To perform the agglutination activity the bacteria Aeromonas hydrophila (CPQBA 228 08) was used.…”

“…This method of administration can be improved by using a booster (second vaccine application) to enhance vaccine durability, antibody production, and immune memory cells (CHENG et al, 2010). The oral vaccine is a good way to provide the booster, because it is practical, low cost, and viable in culture farms (SILVA et al, 2009).…”

Immunization of hybrid surubim (Pseudoplatystoma corruscans x P. fasciatum) against Motile Aeromonas hydrophila septicemia

“…The supernatant was discarded and the bacterial pellet was re-suspended in PBS to get a concentration of 2x10 8 CFU mL -1 , according to the bacterial growth curve (bacterial concentration × absorbance) previously performed (Silva et al 2012). Vaccine concentrations were chosen according to the concentrations used in scientific studies with freshwater fish (Silva et al 2009, Klesius et al 1999, Evans et al 2004, Santos et al 2005, Shoemaker et al 2006). In the experiment, 48 fishes (47±9 g) were distributed in 12 tanks of 100 L in a closed recirculation system with heating.…”

“…The serum was stored frozen at -20ºC until analysis. Agglutination titer and serum antimicrobial activity against A. hydrophila was analyzed in the microplates by serial dilutions (Silva et al 2009). Agglutination titer was expressed by last serum dilution that showed their respective activities.…”