Haplotypes and variable position detection in the mitochondrial DNA coding region encompassing nucleotide positions 10,716–11,184

Abstract

This study evaluates the mitochondrial noncoding regions by using the Sanger sequencing method for application in Forensic Science. FTA® Technology (FTA™ paper DNA extraction) was utilized to extract DNA. Portion of coding region encompassing positions from (10,716 to 11,184) amplified in accordance with the Anderson reference sequence. PCR products purified by EZ-10 spin column were then sequenced and detected using the ABI 3730 × L DNA Analyzer. A new polymorphic positions 10,750 and 10,790 that are described may be suitable sources in future for identification purpose. The data obtained can be used to identify variable nucleotide positions characterized by frequent occurrence, most promising for identification variants.

In Vitro Antibacterial, Antifungal and Phytochemical Analysis of Methanolic Extract of Fruit Cassia Fistula

“…The extract was filtered with Whatman's filter paper. Filtrate was concentrated under reduced pressure and preserved at 5Cº in dark air tight bottles 18,19 .…”

Evaluation of anti-bacterial activity and bioactive chemical analysis of Ocimum basilicum using Fourier transform infrared (FT-IR) and gas chromatography-mass spectrometry (GC-MS) techniques

“…Phytochemicals are naturally occurring and biologically active plant compounds that have potential disease inhibiting capabilities (Rukayadi et al, 2006;Yoshikawa et al, 2007;Hameed et al, 2015a;Al-Marzoqi et al, 2016). It is believed that phytochemicals may be effective in combating or preventing disease due to their antioxidant *Corresponding author.…”

Antibacterial Effect of Ginger (Zingiber officinale) Roscoe and Bioactive Chemical Analysis using Gas Chromatography Mass Spectrum

“…About 1ìL of the methanol extract was injected into the GC-MS using a micro syringe and the scanning was done for 45 minutes 17,18 . As the compounds were separated, they eluted from the column and entered a detector which was capable of creating an electronic signal whenever a compound was detected.…”

“…The identification of phytochemical compounds is based on the peak area, retention time molecular weight, molecular formula, MS Fragment-ions and pharmacological actions. GC-MS analysis of Zingiber officinale revealed the existence of the Octanal, 2-Naphthale namine, 1,2,4a,5,6,7,8, EndoBorneol, Decanal,1,[2][3][4][5][6][7][8][9][10][11][12][13][14][15] Propanal, Benzeneacetic acid ,2,3, 3´,15,16,21,22,(3ß,15á,16á,21ß,4,6a,7,8,9,10,10a,8,1, Naphthalene, decahydro-1-pentadecyl-, 13-Docosenamide,(Z)-, 9,10-Secocholesta-5,7,10(19)-triene-3,24,25-triol, (3ß,5Z,7E)-, n-(2,4-Dinitrophenyl)-N´-13-(2,6,6-trimethyl-cyclohex-1-enyl)propylider, n-(2,4-Dinitrophenyl)-N´-13-(2,6,6-trimethyl-cyclohex-1-enyl)propylider, Ingol 12-acetate, 2,2,8,12,7,11, Piperine,8,12,4, …”