The objectives of this research was to determine the chemical composition of roscoe extract from methanol and evaluation of antibacterial activity. The phytochemical compound screened by GC-MS method. Forty eight bioactive phytochemical compounds were identified in the methanolic extract of Zingiber officinale. The identification of phytochemical compounds is based on the peak area, retention time molecular weight, molecular formula, MS Fragment-ions and pharmacological actions. GC-MS analysis of Zingiber officinale revealed the existence of the Octanal, 2-Naphthale namine, 1,2,4a,5,6,7,8, EndoBorneol, Decanal,1,[2][3][4][5][6][7][8][9][10][11][12][13][14][15] Propanal, Benzeneacetic acid ,2,3, 3´,15,16,21,22,(3ß,15á,16á,21ß,4,6a,7,8,9,10,10a,8,1, Naphthalene, decahydro-1-pentadecyl-, 13-Docosenamide,(Z)-, 9,10-Secocholesta-5,7,10(19)-triene-3,24,25-triol, (3ß,5Z,7E)-, n-(2,4-Dinitrophenyl)-N´-13-(2,6,6-trimethyl-cyclohex-1-enyl)propylider, n-(2,4-Dinitrophenyl)-N´-13-(2,6,6-trimethyl-cyclohex-1-enyl)propylider, Ingol 12-acetate, 2,2,8,12,7,11, Piperine,8,12,4,