In Vitro Antibacterial, Antifungal and Phytochemical Analysis of Methanolic Extract of Fruit Cassia Fistula

Abstract

The identification of phytochemical compounds is based on the peak area, retention time molecular weight, molecular formula, MS Fragment-ions and Pharmacological actions. GC-MS analysis of Cassia fistula revealed the presence of the Oxacyclododecan-2-one, Imidazole ,2-amino-5-[(2-carboxy)vinyl], D-Glucose , 6-O-á-D-galactopyranosyl, 2-Nonanone, Eicosanoic acid , phenylmethyl ester, Phenol , 4-(2-propenyl), Eugenol, Caryophyllene, ß-copaene, Azulene,1,2,3,3a,4,5,6, 2,4, 1a,2,5,5a,6,9,10, Vitamin E and Glycine ,5ß,. The FTIR analysis of Cassia fistula leaves proved the presence of Alkenes, Aliphatic fluoro compounds, Alcohols, Ethers, Carboxlic acids, Esters, Nitro Compounds, Alkanes, Alcohols and Phenol. Cassia fistula was highly active against Aspergillus terreus (6.99±0.29). Methanolic extract of bioactive compounds of Cassia fistula was assayed for in vitro antibacterial activity against eleven pathogenic bacteria by using the diffusion method in agar. The zone of inhibition were compared with different standard antibiotics. The diameters of inhibition zones ranged from 1.00±0.05 to 6.02±0.23 mm for all treatments.

Evaluation of Susceptibility and Resistance of Human Infectious Bacteria and Identification of Bioactive Compounds in Pistacia atlantica, Cassia absus, and Quercus persica

“…The seed methanol extract of C. auriculata, C. absus, and C. fistula showed higher radical scavenging activity than ethyl acetate and hexane extracts [35], which was similar to our results. The methanol extract of Cassia fistula against S. faecalis showed a more inhibitory effect [36]. The results of this group, probably due to differences in the type of species and climate condition, are different from our results.…”

Antimicrobial Testing of Schinus molle (L.) Leaf Extracts and Fractions Followed by GC-MS Investigation of Biological Active Fractions

“…Some papers have reported the experimental data antibacterial activity against S. aureus of isoaromadendrene, epoxide [55]. The ineffectiveness of squalene was reported by Sharma et al [56] in a study of the ethanolic extract from leaves of Syzygium jambos L. (Alston), in which no antibacterial activity occurred during the testing of squalene.…”

Synergistic Effect of Biogenics Derived from Potential Probiotics Together with Zingerone Against Biofilm Formation by Pseudomonas aeruginosa PAO1

“…Furthermore, the exaggerated antibiofouling potential of the combination of CFS of L. fermentum PUM and zingerone might also be due to the presence of more volatile antimicrobial compounds, such as derivatives of piperidine, pyrazine, pyrrole, quinoline, fumaric acid, imidazole and phthalic acid as compared with the combination CFS of Ped. acidilactici BNS5B and zingerone [38,43,45,46,51,53,54]. This is the first study reporting the presence of volatile antimicrobial compounds produced by potential probiotics, L. fermentum and Ped.…”