Evaluation of anti-bacterial activity and bioactive chemical analysis of Ocimum basilicum using Fourier transform infrared (FT-IR) and gas chromatography-mass spectrometry (GC-MS) techniques

Kadhim, Mohanad Jawad; Sosa, Azhar Abdulameer; Hameed, Imad Hadi

doi:10.5897/jpp2015.0366

Cited by 38 publications

(13 citation statements)

References 35 publications

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“…Of all the extracts tested methanol leaf extracts proved to be the best for E. coli with 29.0 mm zone of inhibition while stem extract has the lowest zone of inhibition of 17mm on Intestinal enterococcus. However, these results are in accordance with studies that had reported that the methanol extract of A. paniculata showed antibacterial activity against E. coli [79,80]. Also in another study by Sharma et al [81], it was found that the MIC of Camellia sinensis and Andrographis paniculata extract was found to be 200 μg/mL against exponentially growing VRE.…”

Section: Antibacterial Activity Of a Paniculata Against The Enteric supporting

confidence: 90%

Phytochemical and Antibacterial Properties of Andrographis paniculata (Burn.F.) on Enteric Bacteria Isolated from a Surface Water in Owena, Nigeria

Ayodele

Olalemi

Ogundare

2020

JAMB

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This study was aimed at determining the antibacterial activities of Andrographis paniculata on methicillin and vancomycin resistant enteric bacteria isolated from River Owena, Owena, Nigeria. Water samples were collected weekly from River Owena over a period of six weeks (n = 6). The bacteriological evaluation of the water samples was carried out using standard microbiological method. A. paniculata leaf and stem extracts were prepared using the polar (i.e., methanol) and nonpolar (i.e., n-hexane) solvents. The phytochemical constituents and antibacterial activity of all the extracts of A. paniculata leaf and stem were investigated using standard methods. Results revealed that methanol extracts from A. paniculata contained the maximum amount of phytochemicals when compared to that of the n- hexane extracts. Salmonella and Shigella were both resistant to vancomycin, while they were only susceptible to methicillin once throughout the sampling week. The methanol extracts from A. paniculata showed higher antibacterial activity against the targeted bacterial isolates of enteric origin which include E. coli, faecal coliforms, Shigella, Salmonella and intestinal enterococci. The methanol leaf extract of A. paniculata showed the maximum antibacterial activity against E. coli, Salmonella, Shigella, intestinal enterococci and faecal coliform with zones of inhibition 29.0 mm, 26.00 mm, 21.3 mm, 21 mm and 19 mm respectively. The methanollic stem extract of A. paniculata showed a maximum antibacterial activity against Salmonella with zone of inhibition of 25 mm while n-hexane stem extract exhibited a maximum zone inhibition of 17.6 mm against Salmonella. The minimum inhibitory concentration (MIC) value for all the A. paniculata extracts was at a concentration of 12.5 – 50 ml. The pH of the water ranged from 6.50 to 6.90, temperature ranged from 26 to 31°C. The findings from this study demonstrated that River Owena is highly contaminated with faecal materials and that leaf and stem extracts of A. paniculata may be used as antibacterial agents against methicillin and vancomycin resistant bacteria of enteric origin.

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Section: Antibacterial Activity Of a Paniculata Against The Enteric supporting

confidence: 90%

Phytochemical and Antibacterial Properties of Andrographis paniculata (Burn.F.) on Enteric Bacteria Isolated from a Surface Water in Owena, Nigeria

Ayodele

Olalemi

Ogundare

2020

JAMB

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“…The incorporation of F. cernua into the nanolaminates delayed the weight loss of the fruit, when compared with the nanolaminates without the extract. This tendency in the weight loss during storage, is natural, as the loss occurs mainly by the transpiration process (Souza et al, 2014;Flores-López et al, 2016a;Mohanad et al, 2016). However, a loss higher than 5% is a restricting factor for the fruit marketing and consumption (Aktas et al, 2012).…”

Section: Weight Lossmentioning

confidence: 99%

Application of edible nanolaminate coatings with antimicrobial extract of Flourensia cernua to extend the shelf-life of tomato (Solanum lycopersicum L.) fruit

Salas-Méndez

Vicente

Pinheiro

et al. 2019

Postharvest Biology and Technology

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Edible coatings have potential to reduce postharvest losses of fruit such as tomato. In this study, the effects of nanolaminate coatings incorporated with extracts of Flourensia cernua, an endemic plant of the arid and semiarid regions of Mexico, has been investigated. Ethanol extracts of F. cernua (FcE) were prepared and incorporated into polyelectrolyte solutions of alginate and chitosan. The nanolaminates were characterized by determining the zeta potential, contact angle and water vapor and oxygen permeabilities. Shelf-life analyses (20°C for 15 d) were carried out with uncoated fruit (UCF), nanolaminate coating (NL) and nanolaminate coating with FcE (NL + FcE). Physicochemical analyses, gas exchange rates of O 2 and CO 2 and ethylene production, as well as microbiological analyses of treated fruit were measured. Zeta potential and contact angle measurements confirmed the successful assembly of successive nanolayers of alginate and chitosan, as well as those with F. cernua. The nanolaminate coatings resulted in decreased permeabilities to water and O 2. The best treatment of NL + FcE, extended the shelf-life of fruit by reducing weight loss and microbial growth, reducing gas exchange and ethylene production, and maintaining firmness and color. The NL + FcE treatment are an alternative to extend the shelf-life of tomato fruit.

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“…It was also detected as a major constituent in essential oils and methanol extract. 52,53 The inhibitory effect of O. basilicum on MDA-MB-435 cells could be strongly attributed to their higher contents of methyl eugenol (40.58%) and phytol (6.03%). Methyl eugenol demonstrated cytotoxic and antimigratory effects against the MDA-MB-231 breast cell line.…”

Section: Resultsmentioning

confidence: 99%

Phytochemical Constituents and In-Vitro Anticancer Activity of some Medicinal Plant Extracts against MCF-7 and MDA-MB-435 Human Breast Cancer Cell L

2023

TJNPR

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Breast cancer (BC) is a malignancy, considered as a global and local health hazard. Searching for new naturally occurring anticancer medications with few adverse effects is attracting attention worldwide. This study aimed to screen the phytochemical components of ethanol extracts of eight Sudanese medicinal plants (Ocimum basilicum leaves, Mentha longifolia leaves, Linum usitatissimum seeds, Allium sativum L bulb, Nigella sativa seeds, Piper nigrum L. fruits, Zingiber officinale rhizome, and Foeniculum vulgare seeds), to evaluate and compare their cytotoxic effects against two types of BC cell lines and to estimate their safety toward normal cell lines. The extracts of the chosen plants were obtained using an ultrasonic-aided extraction process, and their components were characterized by GC-MS. The cytotoxic effects of ethanol extracts were tested in vitro against human breast estrogen receptor-positive MCF-7, triple negative MDA-MB-435, and umbilical vein endothelial cells (HUVEC) normal cell lines using the MTT assay. The ethanol extracts were found to be rich in varied phytochemical components. Mentha longifolia and Ocimum basilicum ethanol extracts exerted the greatest activity against MDA-MB-435 with IC50 equal to 0.7437±0.31 and 16.78±2.47µg/ml, respectively. While Linum usitatissimum, Allium sativum, and Nigella sativa ethanol extracts exhibited significant cytotoxic effects on estrogen receptor-positive MCF-7 type of BC cell line with comparable IC50 values of 2.008±0.18, 8.661±0.25 and 10.072±0.18µg/ml, respectively. All ethanol extracts showed weak cytotoxic effects (IC50 ˃ 100µg/ml) against HUVEC with high selectivity index (SI) values. These findings potentiate the need for further in vivo anti-cancer assessments for the active extracts and their components.

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Evaluation of anti-bacterial activity and bioactive chemical analysis of Ocimum basilicum using Fourier transform infrared (FT-IR) and gas chromatography-mass spectrometry (GC-MS) techniques

Cited by 38 publications

References 35 publications

Phytochemical and Antibacterial Properties of Andrographis paniculata (Burn.F.) on Enteric Bacteria Isolated from a Surface Water in Owena, Nigeria

Phytochemical and Antibacterial Properties of Andrographis paniculata (Burn.F.) on Enteric Bacteria Isolated from a Surface Water in Owena, Nigeria

Application of edible nanolaminate coatings with antimicrobial extract of Flourensia cernua to extend the shelf-life of tomato (Solanum lycopersicum L.) fruit

Phytochemical Constituents and In-Vitro Anticancer Activity of some Medicinal Plant Extracts against MCF-7 and MDA-MB-435 Human Breast Cancer Cell L

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