Gold interdigitated triple-microelectrodes for label-free prognosticative aptasensing of prostate cancer biomarker in serum

Ibau, C.; Arshad, M. K. Md; Gopinath, Subash C.B.; M.N, M. Nuzaihan; Fathil, M. F. M.; Estrela, Pedro

doi:10.1016/j.bios.2019.04.048

Cited by 62 publications

(35 citation statements)

References 28 publications

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“…To observe the diffusivity of the electrolyte onto the electrode surface, wettability of the electrode matrix was measured by a 5 µL water drop contact angle observation. The LAG exhibited higher contact angle around (150.2 ± 3)°, PAAMI‐treated LAG exhibited (44.1 ± 3)°, and PtNPs/PAAMI/LAG exhibited (41.9 ± 3) °, as shown in Figure 3e–g, respectively; consequently, these contact angles indicate that the PAAMI‐treated LAG and PtNPs/PAAMI/LAG are more hydrophilic than the bare LAG electrode 15,20…”

Section: Resultsmentioning

confidence: 85%

“…Therefore, these functional groups are crucially desired due to their stable covalent immobilization. The reduced form of graphene called reduced graphene oxide (rGO) is widely incorporated on sensing platforms for developing highly sensitive and selective biosensors 10,17–21. However, the multistage synthesis and use of toxic reductants are required to synthesize or functionalize rGO.…”

Section: Introductionmentioning

confidence: 99%

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A Polyallylamine Anchored Amine‐Rich Laser‐Ablated Graphene Platform for Facile and Highly Selective Electrochemical IgG Biomarker Detection

Barman

Zahed

Sharifuzzaman

et al. 2020

Adv Funct Materials

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Current immunosensors have an insufficient number of binding sites for the recognition of biomolecules, which leads to false positive or negative results. In this research, a facile, cost‐effective, disposable, and highly selective electrochemical immunosensing platform is developed based on cationic polyelectrolyte polyallylamine (PAAMI) anchored laser‐ablated graphene (LAG). Here, for the first time, PAAMI is introduced to stabilize LAG flakes, while retaining the intrinsic thermal and electronic properties of the substrate by noncovalent π–π interaction and electrostatic physical absorption. The sensing platform offers a suitable number of anchoring sites for the immobilized antibodies by providing NH2 functional groups. The proper grafting of PAAMI is confirmed through X‐ray photoelectron spectroscopy and Raman spectroscopy. The immunosensing platform is applied to detect immunoglobulin (IgG) biomarkers as a proof of concept. Under optimized conditions, the sensing platform exhibits a linear range of 0.012–15 and 15–352 ng mL−1 with a limit of detection of 6 pg mL−1 for IgG detection with high selectivity. Based on the analysis, the developed immunosensing platform can be used for point‐of‐care detection of IgG in clinical diagnostic centers. Furthermore, the developed strategy is well suited for the detection of other cancer biomarkers after immobilizing the relevant antibodies.

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Section: Resultsmentioning

confidence: 85%

Section: Introductionmentioning

confidence: 99%

A Polyallylamine Anchored Amine‐Rich Laser‐Ablated Graphene Platform for Facile and Highly Selective Electrochemical IgG Biomarker Detection

Barman

Zahed

Sharifuzzaman

et al. 2020

Adv Funct Materials

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“…A micromachined Teflon tape was adhered on the board around the sensing area, in order to separate one electrochemical cell on the PCB from another, and locally confine the reagents. The impedance spectra were recorded using gold as counter and pseudo-reference electrodes at an open circuit potential (OCP) in the frequency range of 100 kHz to 0.5 Hz, with a 60 mV amplitude in 50 µL of 100 mM PBS (pH 7.4) containing 2 mM of the [Fe(CN) 6 ] 3−/4− redox couple [30]. All electrochemical data were obtained at room temperature (25 °C).…”

Section: Experimental Methodsmentioning

confidence: 99%

Label-Free Electrochemical Detection of S. mutans Exploiting Commercially Fabricated Printed Circuit Board Sensing Electrodes

et al. 2019

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This paper reports for the first time printed-circuit-board (PCB)-based label-free electrochemical detection of bacteria. The demonstrated immunosensor was implemented on a PCB sensing platform which was designed and fabricated in a standard PCB manufacturing facility. Bacteria were directly captured on the PCB sensing surface using a specific, pre-immobilized antibody. Electrochemical impedance spectra (EIS) were recorded and used to extract the charge transfer resistance (Rct) value for the different bacteria concentrations under investigation. As a proof-of-concept, Streptococcus mutans (S. mutans) bacteria were quantified in a phosphate buffered saline (PBS) buffer, achieving a limit of detection of 103 CFU/mL. Therefore, the proposed biosensor is an attractive candidate for the development of a simple and robust point-of-care diagnostic platform for bacteria identification, exhibiting good sensitivity, high selectivity, and excellent reproducibility.

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“…Regarding chronoamperometric biosensors, antibodies [8][9][10], aptamers [11], peptides [12] and enzymes [13] have been used as recognition molecules, and they are based on dual potentiometry. Regarding impedimetric biosensors using IDE, antibodies [24][25][26][27][28] and aptamers [29] have been used as the primary molecular recognition elements, as affinity-based detection requires strong binding and selective biomolecules for impedance measurement. With impedimetric biosensors, measurement is based on the access of a redox probe to the electrode surface, and changes occur when an insulating layer is produced with the recognition element/antigen complex, which reduces the transfer of electrons.…”

Section: Measurement Of Fructosyl Lysine and Ga With An Ide Enzyme Sementioning

confidence: 99%

Development of an Interdigitated Electrode-Based Disposable Enzyme Sensor Strip for Glycated Albumin Measurement

et al. 2021

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Glycated albumin (GA) is an important glycemic control marker for diabetes mellitus. This study aimed to develop a highly sensitive disposable enzyme sensor strip for GA measurement by using an interdigitated electrode (IDE) as an electrode platform. The superior characteristics of IDE were demonstrated using one microelectrode of the IDE pair as the working electrode (WE) and the other as the counter electrode, and by measuring ferrocyanide/ferricyanide redox couple. The oxidation current was immediately reached at the steady state when the oxidation potential was applied to the WE. Then, an IDE enzyme sensor strip for GA measurement was prepared. The measurement of fructosyl lysine, the protease digestion product of GA, exhibited a high, steady current immediately after potential application, revealing the highly reproducible measurement. The sensitivity (2.8 nA µM−1) and the limit of detection (1.2 µM) obtained with IDE enzyme sensor strip were superior compared with our previously reported sensor using screen printed electrode. Two GA samples, 15 or 30% GA, corresponding to healthy and diabetic levels, respectively, were measured after protease digestion with high resolution. This study demonstrated that the application of an IDE will realize the development of highly sensitive disposable-type amperometric enzyme sensors with high reproducibility.

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Gold interdigitated triple-microelectrodes for label-free prognosticative aptasensing of prostate cancer biomarker in serum

Cited by 62 publications

References 28 publications

A Polyallylamine Anchored Amine‐Rich Laser‐Ablated Graphene Platform for Facile and Highly Selective Electrochemical IgG Biomarker Detection

A Polyallylamine Anchored Amine‐Rich Laser‐Ablated Graphene Platform for Facile and Highly Selective Electrochemical IgG Biomarker Detection

Label-Free Electrochemical Detection of S. mutans Exploiting Commercially Fabricated Printed Circuit Board Sensing Electrodes

Development of an Interdigitated Electrode-Based Disposable Enzyme Sensor Strip for Glycated Albumin Measurement

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