Label-Free Electrochemical Detection of S. mutans Exploiting Commercially Fabricated Printed Circuit Board Sensing Electrodes

Dutta, Gorachand; Jallow, Abdoulie A.; Paul, Debjani; Moschou, Despina

doi:10.3390/mi10090575

Cited by 29 publications

(17 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To use PCB electrodes as electrochemical sensors, the electrochemical behaviour of the bare electrodes must be repeatable and reproducible. Due to the low-cleanliness environment of the PCB manufacturing facilities, a level of impurities is present in the electrode surface after the production [ 5 , 9 , 10 ].…”

Section: Resultsmentioning

confidence: 99%

Utilising Commercially Fabricated Printed Circuit Boards as an Electrochemical Biosensing Platform

et al. 2021

Self Cite

View full text Add to dashboard Cite

Printed circuit boards (PCBs) offer a promising platform for the development of electronics-assisted biomedical diagnostic sensors and microsystems. The long-standing industrial basis offers distinctive advantages for cost-effective, reproducible, and easily integrated sample-in-answer-out diagnostic microsystems. Nonetheless, the commercial techniques used in the fabrication of PCBs produce various contaminants potentially degrading severely their stability and repeatability in electrochemical sensing applications. Herein, we analyse for the first time such critical technological considerations, allowing the exploitation of commercial PCB platforms as reliable electrochemical sensing platforms. The presented electrochemical and physical characterisation data reveal clear evidence of both organic and inorganic sensing electrode surface contaminants, which can be removed using various pre-cleaning techniques. We demonstrate that, following such pre-treatment rules, PCB-based electrodes can be reliably fabricated for sensitive electrochemical biosensors. Herein, we demonstrate the applicability of the methodology both for labelled protein (procalcitonin) and label-free nucleic acid (E. coli-specific DNA) biomarker quantification, with observed limits of detection (LoD) of 2 pM and 110 pM, respectively. The proposed optimisation of surface pre-treatment is critical in the development of robust and sensitive PCB-based electrochemical sensors for both clinical and environmental diagnostics and monitoring applications.

show abstract

Section: Resultsmentioning

confidence: 99%

Utilising Commercially Fabricated Printed Circuit Boards as an Electrochemical Biosensing Platform

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…Biosensors combined with laboratory-on-a-chip (LoC) devices, or Point of Care Diagnostics (POCs) offer tremendous advantages such as rapid, portable, high throughput, and automatized detection methods. For considering these issues, an electrochemical detection of Streptococcus mutans was realized with printed-circuit-board (PCB) electrodes [ 35 ]. In this novel PCB based electrochemical biosensor, the thiolated primary antibody was immobilized to the electrode surface and the bacteria detection was realized with EIS.…”

Section: Pathogen Detection With Electrochemical Biosensorsmentioning

confidence: 99%

Pathogen detection with electrochemical biosensors: Advantages, challenges and future perspectives

Kaya

Çetin²,

Azimzadeh

et al. 2021

Journal of Electroanalytical Chemistry

139

View full text Add to dashboard Cite

“…These tools target specific molecules like protein or nucleic acid of the COVID-19 virus with high specificity and sensitivity [ 139 ]. The challenge with biosensors is to convert the complex principle into simpler forms that can not only decrease the cost of technology but also increase the demand in day-to-day life [ 140 , 146 ]. These tools must be user-friendly, as well.…”

Section: Overview Of Biosensing Platforms For Sars-cov-2 Detectionmentioning

confidence: 99%

A comprehensive review on current COVID-19 detection methods: From lab care to point of care diagnosis

Singh¹,

Datta²,

Ashish³

et al. 2021

Sensors International

Self Cite

View full text Add to dashboard Cite

Without a doubt, the current global pandemic affects all walks of our life. It affected almost every age group all over the world with a disease named COVID-19, declared as a global pandemic by WHO in early 2020. Due to the high transmission and moderate mortality rate of this virus, it is also regarded as the panic-zone virus. This potentially deadly virus has pointed up the significance of COVID-19 research. Due to the rapid transmission of COVID-19, early detection is very crucial. Presently, there are different conventional techniques are available for coronavirus detection like CT-scan, PCR, Sequencing, CRISPR, ELISA, LFA, LAMP. The urgent need for rapid, accurate, and cost-effective detection and the requirement to cut off shortcomings of traditional detection methods, make scientists realize to advance new technologies. Biosensors are one of the reliable platforms for accurate, early diagnosis. In this article, we have pointed recent diagnosis approaches for COVID-19. The review includes basic virology of SARS-CoV-2 mainly clinical and pathological features. We have also briefly discussed different types of biosensors, their working principles, and current advancement for COVID-19 detection and prevention.

show abstract

Label-Free Electrochemical Detection of S. mutans Exploiting Commercially Fabricated Printed Circuit Board Sensing Electrodes

Cited by 29 publications

References 31 publications

Utilising Commercially Fabricated Printed Circuit Boards as an Electrochemical Biosensing Platform

Utilising Commercially Fabricated Printed Circuit Boards as an Electrochemical Biosensing Platform

Pathogen detection with electrochemical biosensors: Advantages, challenges and future perspectives

A comprehensive review on current COVID-19 detection methods: From lab care to point of care diagnosis

Contact Info

Product

Resources

About