Glucan and resveratrol complex - possible synergistic effects on immune system

Abstract

Background: Recent data showing that glucan elicited defense responses in grapevine and induced protection via induction of resveratrol production led us to evaluate the possible synergetic eff ects of glucan and resveratrol complex on immune reactions.Methods: We measured phagocytosis using HEMA particles, expression of cell surface markers via fl ow cytometry, expression of cytokines using ELISA, recovery after fl uouracil-induced leucopenia and eff ects on gene expression via RT-PCR.Results: Our results showed that both glucan and resveratrol complex stimulated phagocytosis of blood leukocytes, caused increase in surface expression of CD + splenocytes and showed higher restoration of spleen recovery after experimentally induced leucopenia. In all these cases, strong synergetic eff ects were observed. When we measured the eff ects of these substances on expression level of NF-κB2, Cdc42 and Bcl-2 in breast cancer cells, upregulation of Cdc42 expression was evident only using both immunomodulators in combination.Conlusions: In conclusion, our data suggest signifi cant synergy in stimulation of immune reactions and support further studies of these natural immunomodulators.

Characterization of the novel chemically modified fungal polysaccharides as the macrophage stimulators

“…Stimulated macrophages also release a broad spectrum of cytokines including TNF-α [46]. This observation is in agreement with the recent data provided by Vetvicka et al [47][48][49][50] testifying that various yeast-and mushroom-derived β-D-glucans cause significant potentiation of phagocytic activity, potentiation of synthesis and release of several interleukins and TNF-α, as well as suppression of tumor growth, especially in a synergistic action with another natural immunomodulator-resveratrol [47].…”

The Importance of Gut Microbiota in Pregnancy: Prevention, Care and Support

“…In fact, the activated macrophage consuming and requires ascorbic acid in amounts to 1,000 times higher than those present in the blood. More recent studies have shown that some bioactive molecules, such as vitamin C and resveratrol can further boost the impact of beta-glucan, when given in combination [12,13]. It was observed that their combination suppresses more effectively the growth of mammary tumors and lung, promoting apoptosis of the cancerous cells.…”

EFFECT OF Cayratia trifolia L (Domin) EXTRACT ON REDUCED EXPRESSION OF MATRIX METALLOPROTEINASE-9 (MMP–9) AND VASCULAR ENDOTHELIAL GROWTH FACTOR –A (VEGF-A) IN WHITE RATS WITH BREAST CANCER

“…CYP1B1 role in DMBA metabolism, it also is expressed in organs outside the heart, such as mamma gland. (Gao et al 2003, Vetvicka et al 2007.…”

“…This is because the cells that express MMP-9 in the treatment group was higher than in the normal group. Plant Cayratia trifolia L (Domin) containing the active compound Resveratrol, delphinidinmal-vidin and quercetin content of these chemical compounds have immunomodulatory effects, which potentially inhibit angiopoitin and FGF and decreased angiogenesis, decrease neovascularization, so metastasis in cancer cells can be inhibited (Gao et al 2003, Vetvicka et al 2007.…”