Background: Recent data showing that glucan elicited defense responses in grapevine and induced protection via induction of resveratrol production led us to evaluate the possible synergetic eff ects of glucan and resveratrol complex on immune reactions.Methods: We measured phagocytosis using HEMA particles, expression of cell surface markers via fl ow cytometry, expression of cytokines using ELISA, recovery after fl uouracil-induced leucopenia and eff ects on gene expression via RT-PCR.Results: Our results showed that both glucan and resveratrol complex stimulated phagocytosis of blood leukocytes, caused increase in surface expression of CD + splenocytes and showed higher restoration of spleen recovery after experimentally induced leucopenia. In all these cases, strong synergetic eff ects were observed. When we measured the eff ects of these substances on expression level of NF-κB2, Cdc42 and Bcl-2 in breast cancer cells, upregulation of Cdc42 expression was evident only using both immunomodulators in combination.Conlusions: In conclusion, our data suggest signifi cant synergy in stimulation of immune reactions and support further studies of these natural immunomodulators.
The objective of this study was to find whether the short-time intake of stilbene-resveratrol can influence some haematological indices. The tested compound extracted from the fleeceflower knotweed (Polygonum cuspidatum) was dissolved in saline and administered by a gastric cannula to 15 experimental male rats of Wistar outbreeeding for a week at a daily dose of 4 mg/kg 0.75 of body weight. The effects of resveratrol were compared with 15 control rats that received saline in the same way. The SPF rats (Anlab, Prague) were kept individually in cages and were provided with drinking water and complete feed mixture "Biostan Mypo" ad libitum. The feed contained all necessary energy nutrients, vitamins and minerals for the given animal species and their age. Using a colorimetric method and the Griess agent plasma concentrations of nitrites were determined in blood samples taken at the end of experimental period. Total antioxidative capacity (TRAP) of blood was determined luminometrically and using the aggregometer APACT II employing the turbidimetric method by Born functional tests of platelets were performed. ADP and collagen were employed to induce aggregation. Resveratrol administration did not influence plasma nitrites. No changes were observed in total antioxidative capacity of blood and aggregation characteristics of platelets. However, platelet disaggregation was significantly enhanced (p < 0.01) and their counts significantly decreased (p < 0.01). The results indicate that parameters important for haemostasis can be influenced by the short-time intake of resveratrol administered at the given dose.
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