Effect of Stilbene Resveratrol on Haematological Indices of Rats

Abstract

The objective of this study was to find whether the short-time intake of stilbene-resveratrol can influence some haematological indices. The tested compound extracted from the fleeceflower knotweed (Polygonum cuspidatum) was dissolved in saline and administered by a gastric cannula to 15 experimental male rats of Wistar outbreeeding for a week at a daily dose of 4 mg/kg 0.75 of body weight. The effects of resveratrol were compared with 15 control rats that received saline in the same way. The SPF rats (Anlab, Prague) were kept individually in cages and were provided with drinking water and complete feed mixture "Biostan Mypo" ad libitum. The feed contained all necessary energy nutrients, vitamins and minerals for the given animal species and their age. Using a colorimetric method and the Griess agent plasma concentrations of nitrites were determined in blood samples taken at the end of experimental period. Total antioxidative capacity (TRAP) of blood was determined luminometrically and using the aggregometer APACT II employing the turbidimetric method by Born functional tests of platelets were performed. ADP and collagen were employed to induce aggregation. Resveratrol administration did not influence plasma nitrites. No changes were observed in total antioxidative capacity of blood and aggregation characteristics of platelets. However, platelet disaggregation was significantly enhanced (p < 0.01) and their counts significantly decreased (p < 0.01). The results indicate that parameters important for haemostasis can be influenced by the short-time intake of resveratrol administered at the given dose.

Assessment of the nutritive value of fermented cassava root leaf meal as a reflect on blood profile of ducks

“…The highest value for Hb was observed at diet (T4) followed by diet (T2) having 14.07% and 13.18%. The RBC recorded highest at 2.67% for diet (T3) followed by diet (T4), the value obtained in this study is in agreement with authors (Doubek et al, 2003;Campbell et al, 2013) who reported the recommended value of RBC for ducks to be 2.0 -3.4%. The values observed for white blood cell varied across the treatment, the values vary 10.73 -12.17 with ducks on diet (T3) observed to record the highest value of 12.17% while the least (10.73%) was recorded for birds fed T1.…”

Preventive Effects of Flavonoids on Alloxan-Induced Diabetes Mellitus in Rats

“…The biological activity of food-borne polyphenolics has attracted interest since the work of Bentsáth et al (1936) who proposed that the flavonols were an essential dietary factor contributing to the maintenance of capillary permeability. Although this hypothesis was later abandoned, the growing interest in dietary antioxidants and metabolically active phytochemicals over the last decade has focused attention on other potentially beneficial effects of flavonoids (Hertog and Hollman 1996;Bartošíková et al 2003b;Doubek et al 2005).…”

Contribution to ecological environmental factors and the occurrence of haemosporidians in birds in Zhongar Alatau National Park, Kazakhstan

“…Blood smears were stained according to the Pappenheim method (Doubek et al 2003 ). Microscopic examination of the blood was performed using a Leica DM6000B light microscope (Leica Microsystems, GmbH).…”