Genetic Diversity Analysis of 14 Potato Genotypes Based on Morphological Characters and SSR Markers

Nugroho, Kristianto; Terriyana, Rerenstradika T.; Kusmana, Kusmana; Lestari, Puji; Tasma, I Made

doi:10.21082/jbio.v15n2.2019.p53-64

Cited by 4 publications

(8 citation statements)

References 15 publications

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“…The visualized amplification band is considered as an allele. DNA bands that having the same rate movement is assumed as the same locus (Nugroho et al, 2019). A cross-progeny having the same band pattern with the parents and controlled Jurnal Biodjati 5(1):50-62, May 2020 http://journal.uinsgd.ac.id/index.php/biodjati Al susceptible named by the letter A whereas that which has the same band pattern with the parents and controlled Al tolerant assigned by the letter B.…”

Section: Resultsmentioning

confidence: 99%

Polymorphic Identification of Simple Sequence Repeat (SSR) Marker for Developing Aluminum-Tolerance Upland Rice

et al. 2020

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SSR marker is one of the genetic markers widely applied in plant breeding programs. The application of molecular markers in plant breeding is meant to accelerate the selection of cross-progeny. The research aimed to identify the SSR primers polymorphism between the parent and control that linked to Al tolerance and verify the cross-progeny of five crosses. The result gained from 37 SSR primers used in this study showed that only nine primers are polymorphic. These nine polymorphic primers are RM257, RM214, RM247, RM205, RM490, RM262, RM569, RM271, and RM19. The application of polymorphic markers on five cross-progeny which have shown the same band pattern as the parents and tolerant control on the use of 9 SSR primers recorded as follows: RM257 2 lines, RM214 5 lines, RM247 5 lines, RM205 lines, RM490 13 lines, RM262 5 lines, RM569 7 lines, RM271 4 lines, and RM19 6 lines. The selected SSR primers linked to Al tolerance in this research can be used as a reference for molecular breeding strategies to develop new Al tolerance rice varieties in dryland conditions.

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Section: Resultsmentioning

confidence: 99%

Polymorphic Identification of Simple Sequence Repeat (SSR) Marker for Developing Aluminum-Tolerance Upland Rice

et al. 2020

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“…We use SSR as one of the most widely used marker suitable for MAS [20]. The use of SSR markers on potato has been done on Nugroho et al [13,22] research, and its SSR primer can be open accessed on Indonesian Agricultural Genome Center (Pusat Genom Pertanian Indonesia) [23]. We hope through this present study, in the future, screening and identification of Granola L. variety on potato seed production can be carried out at the upstream level (in vitro planlets).…”

Section: Introductionmentioning

confidence: 99%

“…3 volts for 30 minutes[22].2.2.2. PCR analysis.PCR analysis of 11 genotypes was conducted using 17 SSR primers from PGPI[13] (Table2).…”

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confidence: 99%

Selection of potato molecular markers of Granola L. variety

Sa’adah

Hartanto²,

Sahat³

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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Granola L. is one of potato variety that is very popular in Indonesia and is currently difficult to replace by other varieties. One of the efforts to maintain the genetic purity of the Granola L. variety can be done through biotechnology technique using molecular markers. This research aimed to obtain molecular markers for the Granola L variety. The selection of molecular markers was carried out at the Molecular Biology Laboratory and screenhouse of Indonesian Vegetable Research Institute (IVEGRI) from March to October 2020. The treatments consisted of DNA of Granola L variety from Source Seed Management Unit (UPBS) - IVEGRI, 8 DNA genotypes/germplasm collections which were assumed to be Granola or has a Granola background (Granola BPBK, GM-05, G2, G8, G-771, G-772, GC21, Papita), and 2 DNA genotypes that do not have Granola background (non-Granola) namely Spudi and Amabil varieties. The molecular markers used in this study were 17 SSR primers (Simple Sequence Repeats) from Indonesian Agricultural Genome Center. The results showed that 11 primers were monomorphic and 6 primers were polymorphic. These polymorphic primers still cannot specifically distinguish between Granola L. and non-Granola L. varieties. There is one primer that can distinguish Granola and non-Granola in terms of the band size formed. Further research is needed to find molecular markers that can distinguish Granola L from varieties/genotypes/germplasm collections that are assumed to be Granola L. or having the Granola L. genetic background.

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“…Unbalancing consumption demand and production of potatoes will force the government to continue importing potatoes. Therefore, the development of superior potato varieties with high productivity, disease resistance, and suitability for cultivation in Indonesia needs to be implemented as a current breeding strategy [5].…”

Section: Introductionmentioning

confidence: 99%

“…SSR markers can efficiently facilitate the establishment of genetic linkages due to their high polymorphism level, codominant inheritance, high allele diversity level, randomly wide distribution in the genome, multi-allele, and experimentally reproducible and transferable among related species [9], [10]. Some molecular markers have been applied to the genetic diversity study on potatoes in Indonesia [5], [11]- [15], but only few reported with SSR markers. Therefore, this present study was aimed to quantify the genetic diversity among 29 elite potato varieties in Indonesia and 4 clones that originated from the United States using the 12 SSR markers and to identify the potential genotypes for potato breeding programs.…”

Section: Introductionmentioning

confidence: 99%

Diversity of 33 Genotypes of Potato Revealed by Simple Sequence Repeats Markers

Terryana,

Prihaningsih,

Nugroho

et al. 2022

JOAAT

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Genetic diversity analysis is essential for developing newly high-yielding potato varieties along with other important traits. Simple Sequence Repeats (SSR) markers can be robust tools to assess the genetic diversity of this plant species because they are abundant in the genome, codominant, and more accurate than morphological markers. This study was carried out to quantify the genetic divergence of 29 elite potato varieties in Indonesia and 4 clones that originated from the United States using the 12 SSR markers and identify the potential genotypes for potato breeding programs. A total of 136 gene alleles were detected from 12 SSR markers. The number of alleles per marker ranged from 2 to 22, with an average value of 12.8. All SSR markers showed Polymorphism Information Content (PIC) of 0.66-0.92, with an average of 0.80, and an average value of genetic diversity of 0.82, indicating their high suitability for potato diversity studies. Clustering and principal coordinate analysis classified 33 genotypes into three groups with a coefficient of similarity of 0.76, indicating their high genetic variability. All clones originating from the United States belonged to the same group and separated from the other genotypes. This study gives an overview of the genetic diversity of the Indonesian potato and provides an initial basis of selection for appropriate parents to assist breeders efficiently in developing newly potato varieties with desired agricultural traits in Indonesia.

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Genetic Diversity Analysis of 14 Potato Genotypes Based on Morphological Characters and SSR Markers

Cited by 4 publications

References 15 publications

Polymorphic Identification of Simple Sequence Repeat (SSR) Marker for Developing Aluminum-Tolerance Upland Rice

Polymorphic Identification of Simple Sequence Repeat (SSR) Marker for Developing Aluminum-Tolerance Upland Rice

Selection of potato molecular markers of Granola L. variety

Diversity of 33 Genotypes of Potato Revealed by Simple Sequence Repeats Markers

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