Selection of potato molecular markers of Granola L. variety

Abstract

Granola L. is one of potato variety that is very popular in Indonesia and is currently difficult to replace by other varieties. One of the efforts to maintain the genetic purity of the Granola L. variety can be done through biotechnology technique using molecular markers. This research aimed to obtain molecular markers for the Granola L variety. The selection of molecular markers was carried out at the Molecular Biology Laboratory and screenhouse of Indonesian Vegetable Research Institute (IVEGRI) from March to October 2020. The treatments consisted of DNA of Granola L variety from Source Seed Management Unit (UPBS) - IVEGRI, 8 DNA genotypes/germplasm collections which were assumed to be Granola or has a Granola background (Granola BPBK, GM-05, G2, G8, G-771, G-772, GC21, Papita), and 2 DNA genotypes that do not have Granola background (non-Granola) namely Spudi and Amabil varieties. The molecular markers used in this study were 17 SSR primers (Simple Sequence Repeats) from Indonesian Agricultural Genome Center. The results showed that 11 primers were monomorphic and 6 primers were polymorphic. These polymorphic primers still cannot specifically distinguish between Granola L. and non-Granola L. varieties. There is one primer that can distinguish Granola and non-Granola in terms of the band size formed. Further research is needed to find molecular markers that can distinguish Granola L from varieties/genotypes/germplasm collections that are assumed to be Granola L. or having the Granola L. genetic background.