Gastro-entérite infantile à Rotavirus : Etude épidémiologique, clinique et microbiologique en milieu hospitalier. Approche analytique du diagnostic clinique

Drucker, Jacob; Thompson, R. B.; Fortier, B; Sizaret, P; Nivet, Hubert; Rolland, Jonathan; Grenier, B.

doi:10.1016/s0399-077x(81)80052-2

Médecine et Maladies Infectieuses

1981

DOI: 10.1016/s0399-077x(81)80052-2

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Gastro-entérite infantile à Rotavirus : Etude épidémiologique, clinique et microbiologique en milieu hospitalier. Approche analytique du diagnostic clinique

Jacob Drucker¹,

R. B. Thompson²,

B Fortier

et al.

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1984

1989

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Cited by 4 publications

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“…EM. This was carried out as described by Drucker et al (8). Specimens were diluted to 20% in 0.01 M phosphatebuffered saline (pH 7.3) with 1% Tween 80.…”

mentioning

confidence: 99%

Direct appraisal of latex agglutination testing, a convenient alternative to enzyme immunoassay for the detection of rotavirus in childhood gastroenteritis, by comparison of two enzyme immunoassays and two latex tests

Sambourg¹,

Goudeau²,

Courant³

et al. 1985

J Clin Microbiol

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samples from infants and children with gastroenteritis were tested for rotavirus with four techniques: two enzyme immunoassays (Rotazyme; Abbott Laboratories, North Chicago, Ili., and Enzygnost-Rotavirus; Calbiochem-Behring, La Jolla, Calif.) and two latex agglutination tests (Rotalex; Orion research, Inc., Cambridge, Mass., and Slidex Rota-Kit; Biomérieux). All stool samples were also tested for yeasts and bacterial pathogens. Electron microscopy was used to investigate discrepant results. We found 47% positive samples with Enzygnost-Rotavirus, 38% with Rotazyme, 37% with Slidex Rota-Kit, and 34% with Rotalex. No specimen was found positive py Rotazyme only or Slidex Rota-Kit only. On the contrary, 12 samples which were positive with Enzygnost-Rotavirus only and 3 which were positive with Rotalex only were not confirmed as positive by electron microscopy. Both enzyme immunoassays gave 6% equivocal results; Slidex Rota-Kit gave significantly fewer equivocal results than did Rotalex: 2.9% versus 9.7% (P < 0.01). The sensitivity and specificity of latex tests compared favorably witl that of enzyme immunoassays. Latex agglutination tests can be performed by unskilled personnel and are rapid and relatively cheap. They appear to be very suitable for routine laboratory work and may prove useful for large-scale screening in developing countries. * Corresponding author. MATERIALS AND METHODS Clinical specimens. Two hundred and seven stool samples were collected from children (newborn through 15 years of age) with diarrhea at the Centre Gatien de Clocheville, Tours, France, during February and March 1984. Each stool sample was processed and frozen at-20°C until assayed. The specimens were subjected to only one thawing. All stool samples were tested with four techniques to detect HRV. Two commercially available EIAs (Rotazyme; Abbott Laboratories, North Chicago, Ill., and Enzygnost-Rotavirus; Calbiochem-Behring, La Jolla, Calif.) and two latex agglutination tests, one commercially available (Rotalex; Orion Research Inc., Cambridge, Mass.) and one under development (Slidex Rota-Kit; Biomérieux), were each performed as recommended by the manufacturer. All specimens were also tested for yeasts

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“…EM. This was carried out as described by Drucker et al (8). Specimens were diluted to 20% in 0.01 M phosphatebuffered saline (pH 7.3) with 1% Tween 80.…”

mentioning

confidence: 99%

Direct appraisal of latex agglutination testing, a convenient alternative to enzyme immunoassay for the detection of rotavirus in childhood gastroenteritis, by comparison of two enzyme immunoassays and two latex tests

Sambourg¹,

Goudeau²,

Courant³

et al. 1985

J Clin Microbiol

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Etiologie des gastro-entérites aiguës infantiles en pratique hospitalière courante

Hebert

Caillet

1984

Médecine et Maladies Infectieuses

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Reactogenicity and immunogenicity of rotavirus WC3 vaccine in 5-12-month old infants

Garbag-Chenon

Lasfargues

et al. 1989

Research in Virology

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Gastro-entérite infantile à Rotavirus : Etude épidémiologique, clinique et microbiologique en milieu hospitalier. Approche analytique du diagnostic clinique

Cited by 4 publications

References 12 publications

Direct appraisal of latex agglutination testing, a convenient alternative to enzyme immunoassay for the detection of rotavirus in childhood gastroenteritis, by comparison of two enzyme immunoassays and two latex tests

Direct appraisal of latex agglutination testing, a convenient alternative to enzyme immunoassay for the detection of rotavirus in childhood gastroenteritis, by comparison of two enzyme immunoassays and two latex tests

Etiologie des gastro-entérites aiguës infantiles en pratique hospitalière courante

Reactogenicity and immunogenicity of rotavirus WC3 vaccine in 5-12-month old infants

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