samples from infants and children with gastroenteritis were tested for rotavirus with four techniques: two enzyme immunoassays (Rotazyme; Abbott Laboratories, North Chicago, Ili., and Enzygnost-Rotavirus; Calbiochem-Behring, La Jolla, Calif.) and two latex agglutination tests (Rotalex; Orion research, Inc., Cambridge, Mass., and Slidex Rota-Kit; Biomérieux). All stool samples were also tested for yeasts and bacterial pathogens. Electron microscopy was used to investigate discrepant results. We found 47% positive samples with Enzygnost-Rotavirus, 38% with Rotazyme, 37% with Slidex Rota-Kit, and 34% with Rotalex. No specimen was found positive py Rotazyme only or Slidex Rota-Kit only. On the contrary, 12 samples which were positive with Enzygnost-Rotavirus only and 3 which were positive with Rotalex only were not confirmed as positive by electron microscopy. Both enzyme immunoassays gave 6% equivocal results; Slidex Rota-Kit gave significantly fewer equivocal results than did Rotalex: 2.9% versus 9.7% (P < 0.01). The sensitivity and specificity of latex tests compared favorably witl that of enzyme immunoassays. Latex agglutination tests can be performed by unskilled personnel and are rapid and relatively cheap. They appear to be very suitable for routine laboratory work and may prove useful for large-scale screening in developing countries. * Corresponding author. MATERIALS AND METHODS Clinical specimens. Two hundred and seven stool samples were collected from children (newborn through 15 years of age) with diarrhea at the Centre Gatien de Clocheville, Tours, France, during February and March 1984. Each stool sample was processed and frozen at-20°C until assayed. The specimens were subjected to only one thawing. All stool samples were tested with four techniques to detect HRV. Two commercially available EIAs (Rotazyme; Abbott Laboratories, North Chicago, Ill., and Enzygnost-Rotavirus; Calbiochem-Behring, La Jolla, Calif.) and two latex agglutination tests, one commercially available (Rotalex; Orion Research Inc., Cambridge, Mass.) and one under development (Slidex Rota-Kit; Biomérieux), were each performed as recommended by the manufacturer. All specimens were also tested for yeasts
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