Flow injection solid-phase chemiluminescent immunoassay using a membrane-based reactor

Noncompetitive enzyme immunoassay for carcinoembryonic antigen by flow injection chemiluminescence

“…Under optimal conditions, the CL emission of luminol -H 2 O 2 system was enhanced upon addition of PIP, which accorded with the conclusion reported in Ref. [16]. As seen in Fig.…”

“…To detect HRP at a very low level, several compounds such as phenol derivatives [26,27], carbon dioxide [28], fluorescein [29] and phenylboronic acid [30] have been used to enhance the emission intensity of luminol chemiluminescent system. Although p-iodophenol (PIP) has been used for immunoassay for rabbit IgG [16], study of real-time antigen-antibody interaction [31] and dichlorprop methyl ester [32] and DDT [33] by the enhanced chemiluminescent methods, it has not been used in the determination of tumor marker. The noncompetitive FIIA was proposed in 1993 [23] to detect a hapten by measuring fluorometrically the HRP-labeled antibody -hapten complex, which was separated from the free HRP-labeled antibody by binding the latter to the affinity column.…”

A sensitive immunoassay for determination of hepatitis B surface antigen and antibody in human serum using capillary electrophoresis with chemiluminescence detection

“…Under optimal conditions, the CL emission of luminol-H 2 O 2 system was enhanced upon addition of PIP, which is the one of the broadly used CL enhancers [34]. As seen in Fig.…”

Flow injection immunoassays: A review

“…A thin-layer flow cell for FIA-IA with chemiluminescent detection has been described by Liu et al [34]. This might preclude, for example, the direct analysis of environmental samples.…”