Flow injection immunoassays: A review

Fintschenko, Yolanda; Wilson, George S.

doi:10.1007/bf01246843

Cited by 50 publications

(42 citation statements)

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“…CE/CEC [45][46][47][48] Protein/peptide analysis by CE and related techniques [49][50][51][52] Protein/peptide analysis by CE-MS [53] DNA analysis by CE [54][55][56][57] CE-immunoassay [58,59] FIA-immunoassay [60] Detection for CE [61] Inorganic ions by CE [62] The analysis of small molecules and ions in both nonbiological and biological samples is considered, as are protein, peptide and clinical DNA analysis.…”

Section: Introductionmentioning

confidence: 99%

Microfluidic chips for clinical and forensic analysis

2002

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This review gives an overview of developments in the field of microchip analysis for clinical diagnostic and forensic applications. The approach chosen to review the literature is different from that in most microchip reviews to date, in that the information is presented in terms of analytes tested rather than microchip method. Analyte categories for which examples are presented include (i) drugs (quality control, seizures) and explosives residues, (ii) drugs and endogenous small molecules and ions in biofluids, (iii) proteins and peptides, and (iv) analysis of nucleic acids and oligonucleotides. Few cases of microchip analysis of physiological samples or other "real-world" matrices were found. However, many of the examples presented have potential application for these samples, especially with ongoing parallel developments involving integration of sample pretreatment onto chips and the use of fluid propulsion mechanisms other than electrokinetic pumping.

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Section: Introductionmentioning

confidence: 99%

Microfluidic chips for clinical and forensic analysis

2002

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“…As the labeled antigen has lower affinity to the immobilized antibody, compared to unlabeled analyte in the sample, it is displaced. The intensity of the removed antigen correlates with the amounts of the analyte (Gübitz and Shellum 1993;Fintschenko and Wilson 1998;Bartolomeo and Maisano 2010;Qi and others 2011). Badea and others (2004) used this method to detect AF in fresh milk.…”

Section: Flow Injection Immunoassay (Fiia)mentioning

confidence: 89%

Current Immunoassay Methods for the Rapid Detection of Aflatoxin in Milk and Dairy Products

Matabaro

Ishimwe

Uwimbabazi

et al. 2017

Comp Rev Food Sci Food Safe

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The presence of mycotoxins in foodstuff causes serious health problems to consumers and economically affects the food industry. Among the mycotoxins, aflatoxins are very toxic and highly carcinogenic contaminants which affect the safety of many foods, and therefore endanger human health. Aflatoxin M 1 (AFM 1 ) found in milk results from the biotransformation of aflatoxin B 1 . Many efforts have been made to control the source of AFM 1 from farmers to dairy product companies. However, AFM 1 escapes ordinary methods of food treatment such as cooking, sterilization, and freezing, hence it appears in milk and dairy products. The presence of high levels of AFM 1 constitutes an alarming threat as milk and dairy products contain essential nutrients for human health, especially for infants and children. For this reason, there is a pressing need for developing a fast and reliable screening method for detecting trace aflatoxins in food. Several analytical methods based on high-performance liquid chromatography (HPLC) and mass spectroscopy have been used for aflatoxin detection; however, they are expensive, time-consuming, and require many skills. Recently, immunoassay methods, including enzyme-linked immunosorbent assay (ELISA), immunosensors, and lateral flow immunoassay (LFIA), have been preferred for food analysis because of their improved qualities such as high sensitivity, simplicity, and capability of onsite monitoring. This paper reviews the new developments and applications of immunoassays for the rapid detection of AFM 1 in milk.

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“…as solid supports instead the conventional microtiter plates, and the combination of the immunoassays with flow-through techniques such as the flow injection analysis (FIA) [8,9].…”

Section: Introductionmentioning

confidence: 99%

Recent Advances in Electrochemical Enzyme Immunoassays

2005

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Enzyme immunoassays (EIAs) are currently the predominant analytical technique for the quantitative determination of a broad variety of analytes in clinical, medical, biotechnological, and environmental significance. Although the most common detection methods for EIAs are based on spectroscopic measurements, electrochemical techniques, due to their high sensitivity, selectivity, simplicity and low cost, have emerged as a very attractive alternative to carry out the detection step in this kind of assays. The intention of this review is to cover the progress and development in integrating electrochemical detection methods with EIAs, over the past five years.

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Flow injection immunoassays: A review

Abstract: This review covers the application of flow injection analysis to immunoassays (FIA-IA). The advantages and limitations of this approach are discussed. Future directions, particularly those involving microfluidic systems are highlighted.

Cited by 50 publications

References 50 publications

Microfluidic chips for clinical and forensic analysis

Microfluidic chips for clinical and forensic analysis

Current Immunoassay Methods for the Rapid Detection of Aflatoxin in Milk and Dairy Products

Recent Advances in Electrochemical Enzyme Immunoassays

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