Recent Advances in Electrochemical Enzyme Immunoassays

Díaz‐González, María; González‐García, María Begoña; Costa-Garcı́a, Agustı́n

doi:10.1002/elan.200503357

Cited by 99 publications

(43 citation statements)

References 212 publications

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“…Moreover, the steps of the immunoassay can be carried out by placing a single drop directly on the transducer's surface, which significantly lowers the consumption of expensive reagents, comparatively with other classical methods [20,21]. The modification of the SPCE surface with gold nanoparticles electrochemically generated is a fast method to obtain gold nanostructures in a reproducible way.…”

Section: Introductionmentioning

confidence: 99%

Detection of the peanut allergen Ara h 6 in foodstuffs using a voltammetric biosensing approach

et al. 2015

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A voltammetric biosensor for Ara h 6 (a peanut allergen) detection in food samples was developed. Gold nanoparticle-modified screen-printed carbon electrodes were used to develop a sandwich-type immunoassay using twomonoclonal antibodies. The antibody-antigen interaction was detected through the electrochemical detection of enzymatically deposited silver. The immunosensor presented a linear range between 1 and 100 ng/ml, as well as high precision (inter-day RSD ≤9.8 %) and accuracy (recoveries ≥96.7 %). The detection and quantification limits were 0.27 and 0.88 ng/ml, respectively. It was possible to detect small levels of Ara h 6 in complex food matrices.

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Section: Introductionmentioning

confidence: 99%

Detection of the peanut allergen Ara h 6 in foodstuffs using a voltammetric biosensing approach

et al. 2015

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“…Since the late 1970s, electrochemical devices have received considerable attention in connection to bioaffinity immunosensors [13,14]. Electrochemical immunosensors exploit the selective antibody -antigen binding for triggering useful electrical signals.…”

Section: Electrochemical Bioaffinity Assaysmentioning

confidence: 99%

Nanoparticle‐Based Electrochemical Bioassays of Proteins

Wang

2007

Electroanalysis

140

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This article reviews a variety of new nanoparticle/biomolecule assemblies for advanced electrical detection of proteins. Effective methods for the reliable and trace measurements of proteins are highly desired for facilitating the diagnosis of disease states and improving drug discovery. Ultrasensitive monitoring of biomolecular interactions of proteins is particularly challenging owing to the absence of PCR-like amplification protocols and their greater nonspecific binding to solid supports compared to oligonucleotides. Recent activity has led to innovative and powerful nanoparticle-based immunoassays and aptamer bioassays of proteins based on a variety of electrochemical detection schemes. The enormous signal enhancement associated with the use of nanoparticle amplifying labels and with the formation of nanoparticle-protein assemblies provides the basis for ultrasensitive electrochemical detection of proteins. Such protocols rely on the use of colloidal gold tags, semiconductor (quantum dot) tracers, carrier (amplification) nanomaterials, or magnetic (separation) beads, in connection to electrochemical stripping measurement of the metal tag. Remarkable PCR-like sensitivity has been achieved by coupling particle-based amplification units and various amplification processes. The use of nanocrystal tracers for designing electrochemical coding protocols for detecting multiple proteins will also be documented.

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“…125 Cosnier reviewed the different types of affinity biosensors based on electropolymerized polymer films. The author discussed different fabrication methods for those biosensors (123 citations).…”

Section: Reviewsmentioning

confidence: 99%