A sensitive immunoassay for determination of hepatitis B surface antigen and antibody in human serum using capillary electrophoresis with chemiluminescence detection

Zhang, Yingxue; Zhang, Zhujun; Yang, Feng

doi:10.1016/j.jchromb.2007.07.006

Cited by 31 publications

(28 citation statements)

References 28 publications

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“…Tsukagoshi et al [103] demonstrated a CEIA experiment using mouse IgG and HRPlabeled anti-mouse IgG Ab in a newly designed batchtype CL detection cell for CE. Wang et al [104] [106], hepatitis B surface Ag (HBsAg) and Ab (HBsAb) [107] in human serum.…”

Section: Capillary Electrophoresismentioning

confidence: 99%

Chemiluminescence immunoassay

Zhao

Sun

Chu

2009

TrAC Trends in Analytical Chemistry

187

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Chemiluminescence immunoassay (CLIA) offers several potential advantages and has applications in clinical chemistry, bioanalysis and environmental analysis. This review covers recent developments and applications of CLIA in immunorecognition, CL markers and related techniques, and solid-phase materials. We describe automation, integration and miniaturization of CLIA. We evaluate different CLIA systems and give some pointers as to the likely directions of future developments. ª

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Section: Capillary Electrophoresismentioning

confidence: 99%

Chemiluminescence immunoassay

Zhao

Sun

Chu

2009

TrAC Trends in Analytical Chemistry

187

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“…Hydrogen peroxide is still very used as oxidant in the luminol reaction, in spite of the previously mentioned disadvantages, in conjunction with a catalyst such as a metal ion [97][98][99] or horseradish peroxidase (HRP) [100][101][102][103][104][105][106][107][108]. The preferred metal ions to catalyze the luminol reaction are Co(II) and Cu(II) and both direct and indirect detections are possible.…”

Section: Clinical and Pharmaceutical Applicationsmentioning

confidence: 99%

“…This method allows the baseline separation of HRP complexes from free HRP in less than 3 min, and can be successfully applied to arteriosclerosis pathology research. Similar CEIA methodologies using HRP and p-iodophenol have been used for quantification of CA125 in human sera from health controls and patients associated with ovarian cancer [101], to determine tumor markers, such as a-fetoprotein [104] or carcinoembryonic antigen [108] in human sera samples, to quantify in human serum a luteinizing hormone which control the menstrual cycle [105], or for the determination of clenbuterol in human urine [102] or hepatitis B surface antigen and antibody in human serum [107]. The enhancement effect of p-iodophenylboronic acid can be affected by the presence of mono-and disaccharides in the running buffer and this fact has been used to the molecular recognition of these saccharides [103,112].…”

Section: Clinical and Pharmaceutical Applicationsmentioning

confidence: 99%

Advances and analytical applications in chemiluminescence coupled to capillary electrophoresis

2010

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Advances and analytical applications in chemiluminescence coupled to capillary electrophoresisThe present review presents the state of the art of the developments, key strategies and analytical applications of chemiluminescence detection coupled to CE (CE-CL). Different parts considering the most common CL systems have been included, such as the tris(2,2 0 -bipyridine)ruthenium(II) system, the luminol and derivatives reaction, the peroxyoxalate CL or direct oxidations. New advances in homemade configurations and applications in different fields such as clinical, pharmaceutical, environmental and food analysis have been included. The focus is on studies which appeared from 2000 to the end of 2009.

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“…HRP has been conjugated to both large proteins (i.e., antibodies) and small molecules (i.e., antigens) for use in CE immunoassays [3]. It is most commonly used along with either chemiluminescent detection [88-92] or amperometric detection [93,94]. Examples of analytes that have been detected by CE immunoassays using enzyme labels are hepatitis B surface antigen [88], medroxyprogesterone [89], tumor marker CA125 [90], clenbuterol [91], bone morphogenic protein-2 [92], tumor marker CA15-3 [93], and thyroxine [94].…”

Section: Detection and Labels In Ce Immunoassaysmentioning

confidence: 99%

Capillary electrophoresis‐based immunoassays: Principles and quantitative applications

Moser

Hage

2008

Electrophoresis

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The use of capillary electrophoresis as a tool to conduct immunoassays has been an area of increasing interest over the last decade. This approach combines the efficiency, small sample requirements, and relatively high speed of CE with the selectivity of antibodies as binding agents. This review examines the various assay formats and detection modes that have been reported for these assays, along with some representative applications. Most CE immunoassays in the past have employed homogeneous methods in which the sample and reagents are allowed to react in solution. These homogeneous methods have been conducted as both competitive binding immunoassays and as non-competitive binding immunoassays. Fluorescent labels are most commonly used for detection in these assays, but enzyme labels have also been utilized for such work. Some additional work has been performed in CE immunoassays with heterogeneous methods in which either antibodies or an analog of the analyte is immobilized to a solid support. These heterogeneous methods can be used for the selective isolation of analytes prior to their separation by CE or to remove a given species from a sample/reagent mixture prior to analysis by CE. These CE immunoassays can be used with a variety of detection modes, such as fluorescence, UV/visible absorbance, chemiluminescence, electrochemical measurements, mass spectrometry, and surface plasmon resonance.

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A sensitive immunoassay for determination of hepatitis B surface antigen and antibody in human serum using capillary electrophoresis with chemiluminescence detection

Cited by 31 publications

References 28 publications

Chemiluminescence immunoassay

Chemiluminescence immunoassay

Advances and analytical applications in chemiluminescence coupled to capillary electrophoresis

Capillary electrophoresis‐based immunoassays: Principles and quantitative applications

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