Fibroblast growth factor 2–induced human amniotic mesenchymal stem cells combined with autologous platelet rich plasma augmented tendon-to-bone healing

Abstract

Objective The purpose of this study was to explore the effect of fibroblast growth factor 2 (FGF-2) on collagenous fibre formation and the osteogenic differentiation of human amniotic mesenchymal stem cells (hAMSCs) in vitro, as well as the effect of FGF-2–induced hAMSCs combined with autologous platelet-rich plasma (PRP) on tendon-to-bone healing in vivo. Methods In vitro, hAMSCs were induced by various concentrations of FGF-2 (0, 10, 20, and 40 ​ng/ml) for 14 days, and the outcomes of ligamentous differentiation and osteogenic differentiation were detected by quantitative real-time reverse transcription PCR, Western blot, immunofluorescence, and picrosirius red staining. In addition, a lentivirus carrying the FGF-2 gene was used to transfect hAMSCs, and transfection efficiency was detected by quantitative real time reverse transcription PCR (qRT-PCR) and Western blot. In vivo, the effect of hAMSCs transfected with the FGF-2 gene combined with autologous PRP on tendon-to-bone healing was detected via histological examination, as well as biomechanical analysis and radiographic analysis. Results In vitro, different concentrations of FGF-2 (10, 20, and 40 ​ng/ml) all promoted the ligamentous differentiation and osteogenic differentiation of hAMSCs, and the low concentration of FGF-2 (10 ​ng/ml) had a good effect on differentiation. In addition, the lentivirus carrying the FGF-2 gene was successfully transfected into hAMSCs with an optimal multiplicity of infection (MOI) (50), and autologous PRP was prepared successfully. In vivo, the hAMSCs transfected with the FGF-2 gene combined with autologous PRP had a better effect on tendon-to-bone healing than the other groups ( p ​< ​0.05), as evidenced by histological examination, biomechanical analysis, and radiographic analysis. Conclusion hAMSCs transfected with the FGF-2 gene combined with autologous PRP could augment tendon-to-bone healing in a rabbit extra-articular model. The translational potential of this article hAMSCs transfected with the FGF-2 gene combined with autologous PRP may be a good clinical treatment for tendon-to-bone healing, especially for acute sports-related tendon–ligament injuries.

Human acellular amniotic membrane scaffolds encapsulating juvenile cartilage fragments accelerate the repair of rabbit osteochondral defects

“…In this experiment, hAMSCs were isolated from the amnions of human placentas in accordance with a previous protocol, and relevant informed consent was provided by each donor before the operation. [35][36][37] LG-DMEM/F12 medium containing 10% fetal bovine serum (FBS), 1% penicillin/streptomycin, 1% L-glutamine, and 1% nonessential amino acids was used to culture the hAMSCs. The morphology of the hAMSCs was observed under an inverted microscope after three days of cultivation.…”

Feasibility of Growth Factor Agent Therapy in Repairing Motor Injury

“…GF mimics the clotting physiological events of thrombin-induced fibrin formation and platelet activation and has the key advantage of not having tissue necrosis effects and being biodegradable by body enzymes. Clinically, most of the platelet-rich GF biomaterials are used to achieve wound healing ( Seijas et al, 2014 ), repair soft and hard tissues ( Scala et al, 2014 ), and stimulate bone tissue regeneration ( Zhang et al, 2020 ). They have recently been evaluated in the repair of knee osteoarthritis and muscle damage ( Huang et al, 2020 ), and platelet GF opens up new prospects for regenerative medicine.…”

The effect of stromal vascular fraction and Platelet-Rich Plasma combination on basic Fibroblast Growth Factor serum level during anal trauma healing in a Wistar rat model

“…We contained the blood sample collected from the donor rats within an EDTA-filled tube. We performed a double centrifuge procedure to obtain a PRP [ 20 , 24 ]. First, we centrifuged the sample at 2400 rpm for 10 min, yielding a 3-layered solution.…”

“…Platelet-rich plasma (PRP) exerts its role by stimulating bFGF secretion, which activates fibroblasts and the deposition of new collagen [ 19 ]. The combination of SVF and PRP yields a synergistic effect that improves the healing of osteoarthritis [ 20 ], rat burn injury [ 11 ], and human alopecia [ 21 ].…”