The effect of stromal vascular fraction and Platelet-Rich Plasma combination on basic Fibroblast Growth Factor serum level during anal trauma healing in a Wistar rat model

Sujana, Ricko Sadli; Sulmiati, -; Mariana, Nita; Josh, Fonny; Laidding, Sachraswaty R.; Zainuddin, Andi Alfian

doi:10.1016/j.amsu.2022.103375

Cited by 2 publications

(3 citation statements)

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“…Previous studies have suggested that wound healing will be enhanced by PRP and SVF treatment. 12,14 While the mechanism is not fully understood, its potential components and…”

Section: Discussionmentioning

confidence: 99%

“…The anal trauma model used in this study was adapted from Trebol et al 12,14,16 Sacrificing Procedure Rats from Groups A and B were sacrificed on days 1, 7, and 14 post-treament. All rats were anaesthetized with ether before sacrificed.…”

Section: Anal Trauma Modelmentioning

confidence: 99%

“…The number of rats in each subgroup was four based on Federer's formula. [12][13][14] Twenty-eight rats were divided into three groups. Group A (n=12) was treated with PRP and SVFs (treatment).…”

Section: Wound Analysismentioning

confidence: 99%

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Effects of Stromal Vascular Fraction and Platelet-Rich Plasma Treatment on Capillary Histopathology During Anal Trauma Healing in Rats: An in Vivo Study

Darwin

Sulmiati

Ahmadwirawan

et al. 2023

imjm

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INTRODUCTION: Anal traumas, such as blunt and penetrating injuries to the perineum, can disrupt the anal sphincter and cause considerable morbidity. Non-invasive anal trauma therapies have been widely developed, including growth factors administered as platelet-rich plasma (PRP) and stromal vascular fraction (SVFs) containing adipose[1]derived stem cells (ASCs) and limited growth factor. This study aimed to assess PRP and SVF effects on capillary histopathology in the anal trauma Wistar rat model. MATERIALS AND METHODS: This experimental study used a rat model. Rats were divided into three groups: Group A (n=12) was treated with PRP and SVFs; Group B (n=12) was treated with normal saline (placebo); Group C (n=4) was sacrificed on day 0 to obtain baseline capillary counts. Groups A and B were further divided into three subgroups, sacrificed on days 1, 7, and 14 post-treatment. RESULTS: Capillary counts significantly showed higher density between Groups A and B (p=0.037, analysis of variance). On day 7, there was a significant difference between Groups A and B (p=0.001). CONCLUSION: Mean capillary density significantly improved in anal trauma treated with a combination of PRP and SVFs compared to control. Our findings support the use of PRP and SVFs to aid recovery of patients with anal trauma.

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“…Previous studies have suggested that wound healing will be enhanced by PRP and SVF treatment. 12,14 While the mechanism is not fully understood, its potential components and…”

Section: Discussionmentioning

confidence: 99%

Section: Anal Trauma Modelmentioning

confidence: 99%